| Project/Area Number |
12470441
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
NAKAMURA Seiji Kyushu University, University Dental Hospital, Assoc.Prof., 歯学部附属病院, 講師 (60189040)
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| Co-Investigator(Kenkyū-buntansha) |
SHIRASUNA Kanemitsu Kyushu University, Faculty of Dentistry, Prof., 歯学研究院, 教授 (30093420)
KISHIHARA Kenji Kyushu University, Medical Institute of Bioregulation, Res.Assoc., 生体防御医学研究所, 助手 (80214774)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 2002: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2000: ¥7,200,000 (Direct Cost: ¥7,200,000)
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| Keywords | Immunology / Oral squamous cell carcinoma / Cytotoxic T cell / T cell receptor / Cytokine / Tumor-associated antigen / Gene cloning / Vaccination therapy / 細胞障害性T細胞 |
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| Research Abstract |
Immune response mainly by T cells is considered to play a central role in the host defense mechanisms against tumor cells. This study was addressed to identify T cell receptor (TCR) and recognizing antigen of T cells against oral squamous cell carcinoma. Results obtained in this research were as follows.
1)Identification of TCR of anti-tumor T cells : PCR-based analysis revealed that restricted Vβ gene families are frequently used in tumor-infiltrating lymphocytes. Further SSCP-based analysis found oligoclonal expansion of T cell clonotypes in some Vβ gene families. Almost same results were obtained by analyses in regional lymph nodes.
2)In vitro induction of T cells recognizing squamous cell carcinoma antigen recognized by T cells-1 (SART-1): In vitro induction of T cells recognizing SART-1 was successful by culture of peripheral blood mononuclear cells with SART-1 peptides. TCR Vβ genes used by the T cells were analyzed by same methods as mentioned above and similar T cell clonotypes were found to be included.
DNA sequencing of TCR Vβ genes used by the T cells recognizing SART-1 is further being performed, and these results will be useful for the establishment of vaccination therapy with SART-1 and also the estimation of its efficacies.
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