| Co-Investigator(Kenkyū-buntansha) |
ODAJIMA Tetsuyo Sapporo Medical University, School of medicine, Assistant Professor, 医学部, 講師 (00177239)
YOSHIDA Kouichi Sapporo Medical University, School of medicine, Professor, 医学部, 助教授 (60117653)
YAMAGUCHI Akira Sapporo Medical University, School of medicine, Instructor, 医学部, 助手 (10210353)
YAMADA Shunpei Tokyo Medical and Dental University, Graduate School, Instructor, 大学院・総合研究部, 助手 (60302890)
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| Budget Amount *help |
¥16,300,000 (Direct Cost: ¥16,300,000)
Fiscal Year 2001: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥13,100,000 (Direct Cost: ¥13,100,000)
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| Research Abstract |
Immunohistochemical examination of the cases with oral squamous cell carcinoma with pRb2/p130 showed that positive staining for pRb2/p130 was observed in many of the cases with well differentiated tumor and clinically low malignant cases in which a metastatic lesion was not found. Expresdion of pRb2/p130 protein may be a good prognostic indicator ui patients with oral squamous cell carcinoma.
Imuunohistochemical examination of the cases with oral squamous cell carcinoma with E-cadherin, α-catenin, βcatenin, and Epithelial Growth Factor Receptor (BGFR), which are cell-adhesion related factors, revealed that die expressions of these factors were found to be correlated with die existence of the regional metastasis, meaning that these expressions were reduced in many of the cases with the metastatic lesion. Expression of p53 protein, which is proliferation-related factor, was not correlated with the existence of the regional metastatic lesion Mutation of β-catenin was not found in tte area of exon-3.
In established cell lines derived from oral cancer aberrant methylation of the genes such as p16INK4A, DAPK, DOC, MINT1, MINT2, MINT27, and MINT31 was found and in human tongue carcinoma tissues that such as p14ARF, p15INK4B, p16INK4A, DAPK, DOC, MINT1, MINT2, MINT27, and MINT31 was found. Aberrant mefttylation of p14ARF was found in many of wefl differentiated tongue carcinomas.
We constructed RGD-fiber modified adenovirus vector and by this vector gene transduction efficiency was enhanced in vivo and in vitro. In normal tissues, gene transduction was not recognized in case of using this vector.
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