• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Bone forming capacity of bone marrow stem cells three-dimentionally cultured with biodegradable scaffolds and growth factors

Research Project

Project/Area Number 12470450
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionKanagawa Dental College

Principal Investigator

KINOSHITA Yukihiko  Kanagawa Dental College, Frontier Oral Science, Professor, 高次口腔科学研究所, 教授 (70084770)

Co-Investigator(Kenkyū-buntansha) KAWASE Toshio  Kanagawa Dental College, School of Dentistry, Professor, 歯学部, 教授 (30084784)
OZONO Satoru  Kanagawa Dental College, Frontier Oral Science, Associate Professor, 高次口腔科学研究所, 助教授 (40084785)
TABATA Yasuhiko  Kyoto University, Frontier Medical Science, Professor, 再生医科学研究所, 教授 (50211371)
YOKOYA Shigetoshi  Kanagawa Dental College, School of Dentistry, Lecturer, 歯学部, 講師 (60267523)
NEGISHI Hideyuki  Kanagawa Dental College, School of Dentistry, Lecturer, 歯学部, 講師 (60121026)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥10,500,000 (Direct Cost: ¥10,500,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2000: ¥6,700,000 (Direct Cost: ¥6,700,000)
Keywordsbone formation / mesenchymal stem cell / biodegradable scaffold / collagen / PGA sponge / Dexamethasone / bFGF / three dimensional culture / scaffold / PGA / colla-gen / β-TCP / 骨形成 / dexamethasone / 生体吸収性材料 / 骨形成能 / 骨髄骨源性幹細胞 / cell culture / 三次元培養骨
Research Abstract

1. Fabrication of biodegradable scaffolds. A collagen solution with poly (glyconic acid) (PGA) fiber was freeze-dried, followed by hydrothermal cross-linking to obtain novel collagen sponges incorporating PGA fiber (PGA/collagen sponge). The mechanical strength and the degree of cellular infiltration to this sponge are greater than collagen sponge alone. Further, PGA/collagen sponge is stronger after UV irradiation.
2. Effects of dexamethasone (Dex) and bFGF on proliferation and differentiation of bone marrow mesenchymal stem cells (BMSC). In single layer cultures, it was shown that dexamethasone and bFGF promote respectively ALPase activity and cell proliferation, and simultaneous combination of them showed a multiplier effect in the ALPase activation and remarkable bone nodule formation.
3. Proliferation and differentiation of BMSC on the biodegradable scaffolds
1)BMSCs of rats (RBMSc) were three-dimensionally cultured in
PGA/collagen sponges and PGA/collagen (UV) sponges. The results sh … More owed that shrinkage of both sponges was slight, their pore structures maintained well, and cells infiltrated into the inside of the sponges. In particular, collagen was left more abundantly, and cellular adhesion in pores and the formation of extra cellular matrix were better in collagen PGA/collagen (UV) Sponge. Further, PGA/collagen (UV) sponge showed higher cell proliferation and ALPase activity, and it had greater utility as a scaffold.
4. Bone formation of 3D-cultured BMSC.(1) When RBMSCs/β-TCP hybrid materials cultivated
osteogenic media were transplanted subcutaneously under the back skin of close relative rats, RBMSCs/β-TCP cultured in a medium supplemented with Dex, β-glycerophosphate and bFGF showed good bone formation. 2)When the above BMSCs/β-TCP hybrid materials were autografted into the mandibular continuity defects of adult dogs, the bone-continuity could not be restored. Experience to determine the suitable cultivation period of BMSCs and the method of local application of bioactive factors promoting bone formation will be done in the future. Less

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] 田畑泰彦: "生体適合性材料と再生医療.21世紀における再生医療の役割"BIO INDUSTRY. 17. 5-11 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 田畑泰彦: "再生医学の応用の現状と課題.マトリックスとDDS"Pharma. Medica.. 18. 69-74 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasuhiko Tabata: "Significance of biomaterials and drug delivery systems in tissue engineering"Connective Tissue. 33. 315-324 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 木下靭彦: "特集、再生医療の現状と将来展望.顎骨の再生医療"日本医師会雑誌. 129. 333-335 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 木下靭彦: "顎骨の再生医療-生体吸収性ポリ-L-乳酸メッシュと新鮮自家骨髄海綿骨細片を用いた下顎骨再生-"日本歯科医師会雑誌. 22. 107-110 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yukihiko Kinoshita: "Methods of Tissue Engineering"Anthony Atala arid Robert P.Lanza, Academic Press 2002. 1, pp.. 1195-1204 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yukihiko Kinoshita: "Regenerative medicine in Maxillary and mandibular bone -Regeneration of the mandible with Poly (L-lactic acid) mesh and autogenic particulate, cancellous bone and marrow"JJDS. 22. 107-110 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasuhiko Tabata, et al.: "Significance of biomaterials and drug delivery systems in tissue engineering"Connective Tissue. 32. 315-324 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shigetoshi Yokoya, et al.: "Tissue engineering in jowbone -Potential efficacy of bFGF-incorporated gelatin microspheres for bone formation of mandibulardefects -"J.Stomatol.Soc.. 51. 324-334 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiraoka Yosuke, et al.: "Fabrication and biocompatibility of collagen Sponge reinforced with poly (glycolic acid) fiber"Tissue Engineering. 9. 1101-1112 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yukihiko Kinoshita: "Regenerative medicine for Jawbone"JMAJ. 333-335 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yukihiko Kinoshita, et al.: "Jawbone, Methods of tissue engineering"Anthony Atala, Robert P.Lanza, Academic Press. 1195-1204 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 横矢重俊: "bFGF含浸ゼラチン粒子の顎骨欠損部骨再生に及ぼす影響"日本口腔科学会誌. 51・5. 324-334 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 木下靭彦: "再生医療の現状と将来展望.顎骨の再生医療"日本医師会雑誌. 129・3. 333-335 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小園 知: "ラット骨髄間葉系細胞/β-TCP多孔体顆粒複合体の骨形成能におけるβbFGFの効果について"歯科基礎歯科医学会雑誌. 44・5. 124-124 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 藤田元規: "ポリグリコール酸・コラーゲンスポンジを足場としたラット骨髄間葉系幹細胞の三次元培養について"日本歯周病学会雑誌. 44・supple. 151-151 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 木下靭彦: "再生医療の実際"羊土社. 7 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yasuhiko Tabata: "Significance of biomaterials and drug delivery systems in tissue engineering"Connective Tissue. 33. 315-324 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yukihiko Kinoshita: "Tissue Engineering of Jaw Bone-Potential Efficacy of bFGF-Incorporated Gelatin Microspheres for Bone Formation of Mandibular Defect"Int. J. Oral. Maxillofac. Surg. 30. 110-110 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tadashi Yamazaki: "Repair of Human Orbital Floor Defects Using Poly [L-lactide] Mesh Sheets Filled with Particulate Cancellous Bone and Marrow"Int. J. Oral. Maxillofac. Surg. 30. 92-92 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 田畑泰彦: "細胞増殖因子の徐放化技術を用いた骨再建"THE BONE. 15(5). 45-48 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 川瀬俊夫: "歯周組織におけるティシュエンジニアリングの有効性と展望"日本歯周病学会会誌. 43. 347-352 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yukihiko Kinoshita: "Jawbone, Methods of Tissue Engineering"Academic Press. (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 田畑泰彦: "生体適合性材料と再生医療・特集21世紀における再生医療の役割"BIO INDUSTRY. 17・1. 5-11 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 田畑泰彦: "再生医学の応用の現状と課題,マトリックスとDDS"Pharma Medica. 18・1. 69-74 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 木下靭彦: "科学フロンティア(3)「再生医工学」"化学同人社(印刷中). (2001)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi