| Project/Area Number |
12470471
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
TAKASHIBA Shogo Okayama University, Graduate School of Medicine and Dentistry, Department of Pathophysiology - Periodontal Science, Associate Professor, 大学院・医歯学総合研究科, 助教授 (50226768)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Hiroshi Okayama University, Graduate School of Medicine and Dentistry, Department of Pathophysiology - Periodontal Science, Instructor, 大学院・医歯学総合研究科, 助手 (00274001)
MYOKAI Fumio Okayama University, Graduate School of Medicine and Dentistry, Department of Pathophysiology - Periodontal Science, Instructor, 大学院・医歯学総合研究科, 助手 (50263588)
NISHIMURA Fusanori Okayama University, Graduate School of Medicine and Dentistry, Department of Pathophysiology - Periodontal Science, Assistant Professor, 歯学部・附属病院, 講師 (80208222)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 2001: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 2000: ¥6,100,000 (Direct Cost: ¥6,100,000)
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| Keywords | Periodontal disease / TNF / Regulation of Gene Transcription / Local Gene Delivery / Anti-inflammatory Therapy |
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| Research Abstract |
Lipopolysaccharide (LPS) is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor alpha (TNF-α). It gives the deleterious effects to the host of TNF-α, especially advance of inflammation, the destruction of the tissue. It also gives same effects in the periodontal tissue.
We tried to control TNF-α gene transcription not using NF-κB strongly concerned with other activities of the cells but using LITAF (LPS-induced TNF-α factor) which we identified as a new transcription factor of its transcription. We found that inhibition of LITAF mRNA expression in THP-1 cells resulted in a reduction of TNF-α transcription.
We could find strong LITAF promoter region by LPS stimulation and same consensus sequences as NF-IL6 and AP-1 in this region. On the other hand, we found LITAF localization because staining for LITAF was present in the nuclei of THP-1 after LPS stimulation. We also established gene transfection to find the best method for transfection in vivo.
In addition, we transfected mutants of 1κB-α which is one of the inhibitor of NF-κB into the THP-1 cells. We established only the sysyem of THP-1 cells without NF-κB influence, however we will discussed the influence of TNF-α expression by LITAF and the effect of LITAF without NF-κB even though this research period was over.
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