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Development of therapeutic ways on various pathological features in chronic obstructive pulmonary diseases

Research Project

Project/Area Number 12470527
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用薬理学・医療系薬学
Research InstitutionKumamoto University

Principal Investigator

MIYATA Takeshi  Kumamoto University, Graduate School of Pharmaceutical Sciences, Department of Chemico-Pharmacological Sciences, Professor, 大学院・医学薬学研究部, 教授 (90040310)

Co-Investigator(Kenkyū-buntansha) ISOHAMA Yoichiro  Kumamoto University, Graduate School of Pharmaceutical Sciences, Department of Chemico-Pharmacological Sciences, Associate Professor, 大学院・医学薬学研究部, 助教授 (10240920)
KAI Hirofumi  Kumamoto University, Graduate School of Pharmaceutical Sciences, Department of Molecular Medicine, Professor, 大学院・医学薬学研究部, 教授 (30194658)
TOKUTOMI Naofumi  Kumamoto University, Graduate School of Medicine, Department of Pharmacology, Associate Professor, 大学院・医学薬学研究部, 助教授 (30227582)
FUKUNAGA Kohji  Graduate School of Pharmaceutical Sciences, Tohoku University Department of Pharmacology, Professor, 大学院・薬学研究科, 教授 (90136721)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 2002: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2000: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsETS transcription factor / airway mucin / GM-CSF / pulmonary surfactant / lung / epithelial cells / 気道上皮細胞 / 慢性閉塞性呼吸器疾患 / グリチルリチン / prostanlandin E_2 / 11β-hydroxysteroid dehydrogenase / 肺胞上皮細胞 / 細胞分化 / MEF / ETS-2 / 漢方薬
Research Abstract

Chronic obstructive pulmonary disease (COPD) is a major cause of morbidity in the world. However, there are only a few therapeutic drugs for the treatment of this disease. To evaluate new pharmacological approaches for COPD, it is necessary to make progress in understanding lung physiology and pathophysiology of COPD. The findings in this study were summarized below.
1. We have found that myeloid Elf-1 like factor (MEF) regulated the differentiation of airway epithelial cells. In addition to this, MEF-transfected carcinoma cells did not grow. Therefore, we proposed that MEF act as not only a differentiation regulator, but also a tumor suppressor.
2. We found that GM-CSF mRNA expression was upnregulated by ETS-2 transcription factor in adenocarcinoma cells, and was a target of protein kinase C.
3. We have found some new drugs to control mucous secretion in airway diseases. Among them, fudosteine, a new cysteine derivative, inhibited the formation of airway goblet cells, and glycyrrhizin, inhibited mucus production by enhancing the effect of glucocorticoids.
4. We have found new endogenous pulmonary surfactant secretagogues, adrenomedullin and prostaglandin E_2. We also found that there is a synergistic cross-talk between protein kinase A- and protein kinase C-dependent signalings in alveolar type II cells.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Okumura M ほか: "Adrenomedullin increases phosphatigylcholine secretion in rat type II pneumocytes"Eur.J.Pharmacol.. 403. 189-194 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 高橋光一 ほか: "ウサギおよびラット気道分泌に対する新規システイン誘導体フドステインの影響"日薬理誌. 116. 371-378 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Isohama Y ほか: "Bakumondo-to (Mai-Men-Dong-Tang) increases β_1-adrenergic receptor mRNA expression in rat alveolar type II cells"J.Trad.Med.. 18. 8-14 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Isohama Y ほか: "Bakumondo-to (Mai-Men-Dong Tang) increases intracellular cAMP in alveolar type II cells : Bakumondo-to stimukates production and inhibits degradation of cAMP"J.Trad.Med.. 18. 15-19 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Isohama Y ほか: "Interaction between β-adrenergic signaling and protein kinase C increases cytoplasmic Ca^<2+> in alveolar type II cells"Life Sciences. 68. 2361-2371 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Isohama Y ほか: "Glucocorticoid enhances the response of type II cells from newborn rats to surfactant secretagogues"Biochim.Biophys.Acta.. 1531. 241-250 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Morsy MA ほか: "Prostaglandin E_2 increases surfactant secretion via the EP_1 receptor in rat alveolar type II cells"Eur.J.Pharmacol.. 426. 21-24 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagayama S ほか: "Carbenoxolone, a new inducer of heat shock protein 70"Life Sciences. 69. 2867-2873 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suico MA ほか: "Functional dissection of the ETS transcription factor MEF"Biochim.Biophys.Acta.. 1577. 113-120 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Isohama Y ほか: "Glucocorticoid-loke and glucocorticoid-unlike regulation of gene expression by Bakumondo-to (Mai-Men-Dong-Tang) in airway epithelial cells"Jpn.J.Orient.Med.. 53. 1-9 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Seki Y ほか: "The ETS transcription factor MEF is a candidate tumor suppressor gene on the X chromosome"Cancer Res.. 62. 6579-6586 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okiyoneda T ほか: "Calnexin Delta 185-520 partially reverses the misprocessing of the Delta F508 cystic fibrosis transmembrane conductance regulator"FEBS Lett.. 526. 87-92 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 礒濱洋一郎 ほか: "肺サーファクタントの分泌におけるprostaglandin E_2の役割"分子呼吸器病. 7. 207-211 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lu Z. ほか: "EIS2 is involved in protein kinase C-activated expression of granulocyte-macrophage colony-stimulating factor in human non-small lung carcinoma cell line A549"Biochem.Biophys.Res.Com.. 303. 190-195 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okumura M, Kai H, Arimori K, Iwakiri T, Hidaka M, Shiramoto S, Isohama Y, Miyata T.: "Adrenomedullin increases phosphatigylcholine secretion in rat type II pneumocytes."Eur. J. Pharmacol.. 403. 189-194 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi K, Iwase N, Ichikawa M, Mizuno H, Koda T, Kai H, Miyata T.: "Effects of fudosteine, a new cysteine derivative, on airway secretion in rabbits and rats"Nippon Yakurigaku Zasshi. 116. 371-378 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Isohama Y, kanemaru M, Kai H, Takahama K, Miyata T.: "Bakumondo-to (Mai-Men-Dong-Tang) increases β_1-adrenergic receptor mRNA expression in rat alveolar type II cells"J. Trad Med.. 18. 8-14 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Isohama Y, kanemaru M, Kai H, Takahama K. Miyata T.: "Bakumondo-to (Mai-Men-Dong-Tang) increases intracellular cAMP in alveolar Type II cells: Bakumondo-to stimulates production and inhibits degradation of cAMP."J. Trad. Med.. 18. 15-19 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Isohama Y, kanemaru M, Kai H, Takahama K, Miyata T.: "Interaction between β-adrenagic signaling and protein kinase C increases cytoplasmic Ca^<2+> in alveolar type II cells."Life Sciences. 68. 2361-2371 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Isohana Y, Rooney SA.: "Glucocorticoid enhances the response of type II cells from newborn rate to surfactant secretagogues."Biochin. Biophys. Acta.. 1531. 241-250 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Morsy MAM, Isohama Y, Miyata T.: "Prostaglandin E_2 increases surfactant secretion via the EP_1 receptor in rat alveolar type II cells."Eur. J. Pharmacol.. 426. 21-27 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagayama S , Jono H, Suzaki H, Sakai K, Tsuruya E, Yamatsu I, Isohama Y, Miyata T, Kai H.: "Caibenoxolone, a new inducer of heat shock protein 70."Life Sckaices. 69. 2867-2873 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suico MA, Koyanagi T, Ise S, Lu Z, Hisatsune A, Seki Y, Shuto T, Isohama Y, Miyata T, Kai H.: "Functional dissection of the ETS transctiption fector MEF."Biochim. Biophys. Acta.. 1577. 113-120 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Isohama Y, Moriuchi H, Kai H, Miyata T.: "Glucocoryicoid-like and glucocorticoid-unlike regulation of gene expression by Bakumondo-to (Mai-Men-Dong-Tang) in airway epithelial cells."Jpn. J. Orient. Med.. 53. 1-9 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Seki Y, Suico MA, Uto A, Hisatsune A, Shuto T, Isohama Y, Kai H.: "The ETS transcription factor MEF is a candidate tumor suppressor gene on the X chromosome."Cancer Res.. 62. 6579-6586 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okiyoneda T, Wada I, Jono H, Shuto T, Yoshitake K, Nakano N, Nagayanma S, Harada K, Isohama Y, Miyata T, Kai H.: FEBS Lett.. 526. 87-92 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Isohama Y, Morsy MAM, Hisatune A, Miyata T.: "The role of prostaglandin E_2 in pulumonary surfactant secretion."Bunshi-Kokyukibyou. 7. 207-211 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lu Z, Kim KA, Suico MA, Uto A, Seki Y, Shuto T, Isohama Y, Miyata T, Kai H.: "ETS2 is involved in protein kinase C-activated expression of granulocyte-macrophage colony-stimulating factor in human non-small lung carcinoma cell line A549."Biochem. Biophys. Res. Com.. 303. 190-195 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suico MA, Isohama Y, Miyata T, Kai H.: "Functional dissection of the ETS transcription factor MEF"Biochim Biophys Acta.. 1577. 113-120 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Okiyoneda T, Isohama Y, Miyata T, Kai H.: "Calnexin Delta 185-520 partially reverses the misprocessing of the Delta F508 cystic fibrosis transmembrane conductance regulator"FEBS Lett.. 526. 87-92 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Seki Y, Suico MA, Uto A, Hisatsune A, Shuto T, Isohama Y, Kai H.: "The ETS transcription factor MEF is a candidate tumor suppressor gene on the X chromosome"Cancer Res.. 62. 6579-6589 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Isohama Y., Rooney, SA: "Glucocorticoid enhances the response of type II cells from newborn rats to surfactant secretagogues"Biochim Biophys Acta. 1531. 241-250 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Isohama Y, Kanemaru M, Kai H, Takahama K, Miyata T.: "Interaction between β-adrenergic signaling and protein kinase C increases cytoplasmic Ca^<2+> in alveolar type II cells"Life Sciences. 68. 2361-2371 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Morsy MA, Isohama Y, Miyata T.: "Prostaglandin E_2 increases surfactant secretion via the EP_1 receptor in rat alveolar type II cells"Eur J Pharmacol. 426. 21-24 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nagayama S, Isohama Y, Miyata T, Kai H. ほか: "Carbenoxolone, a new inducer of heat shock protein 70"Life Sciences. 69. 2867-2873 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Isohama,M.Kanemaru,H.Kai,K.Takahama and T.Miyata: "Interaction between β-adrenergic signaling and protein kinase C increases cytoplasmic Ca^<2+> in alveolar type II cells."Life Sciences. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Isohama and S.A.Rooney: "Glucocorticoid enhances the response of type II cells from newborn rats to surfactant secretagogues."Biochimica et Biophysica Acta. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takahashi K., et al.: "Effects of fudosteine, a new cysteine derivative, on airway secretion in rabbits and rats"Nippon Yakurigaku Zasshi. 116・6. 371-378 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Okumura M., et al.: "Adrenomedullin increases phosphatidylcholine secretion in rat type II pneumocytes"European Journal of Pharmacology. 403. 189-194 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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