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Functional Assignment among Chromatin Chaperones

Research Project

Project/Area Number 12480186
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionUniversity of Tsukuba (2001)
Tokyo Institute of Technology (2000)

Principal Investigator

NAGATA Kyosuke  Univeiaty of Tsukuba, Institute of Basic Medical Sciences, Professor, 基礎医学系, 教授 (40180492)

Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥10,300,000 (Direct Cost: ¥10,300,000)
Fiscal Year 2001: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2000: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsChromatin / Transcription / Replication / Chaperone / Nucleolus / Nucleo-cytoplasmic shuttling / RNP / Cell cycle / 核一細胞質間輸送 / アデノウイルス / ヒストン / クロマチンリモデリング / cDNAアレー / SAGE法
Research Abstract

The aim of this study is to systematically identify chromatin chaperones involved in chromatin remodeling and to clarify the regulatory mechanism of their activities and the functional assignment among them. We have identified Template Activating Facor (TAF)-III in addition to previously identified TAF-I and TAF-II/Nucleosome Assembly Protein-I by reconstitution and dissection of cell free replication and transcription systems with the adenovirus DNA complexed with viral basic core proteins astemplate. TAF-III was shown identical with nucleophosmin/B23. Analyzes with mutated proteins revealed that the highly acidic amino region in three proteins is essential for their chromatin remodeling activity. We proposed the term "acidic molecular chaperone" for these proteins. Each TAF-I or TAF-III consists of two subtypes, one of which has much less activity than the other. We showed the possibility that the ratio of these two subtypes in a cell is involved in the activity level. The chromatin … More remodeling activity of TAF-I and TAF-III was phosphorylated and markedly reduced : during the mitotic phase. We showed that phosphorylation by a mitotic-specific protein kinase including cyclin B/cdc2 negatively regulates their chromatin remodeling activity. It was indicated that TAF-I and TAF-III exist in nucleus and nucleolus, respectively, while TAF-II was found a nucleocytoplasmic shuttling protein. TAF-I was involved in not only the adenovirus chromatin but also the cellular type chromatin reconstituted with purified histones and plasmid DNA. Genetic experiments using yeast cells indicated that Shuttling but not nuclear localization is important for mitotic progression. Chromatin immunoprecipitation assays showed that TAF-III is associated with the ribosome DNA region. Of interest, is that by functuional analysis of influenza virus ribonucleoproteins, we identified RAF (RNA polymerase Activating Factor)-1 and RAF-2p48/NPI 5/BAT-1/UAP56 as a family member of the acidic molecular chaperone. Thus, it is suggested that proteins categorized into this family involves in function regulation of not only DNA-protein complexes but also RNA-protein complexes. Less

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Mitsuru Okuwaki: "Identification of mucleophosmin/B23, an acidic nuclear protein, as a stimulatory factor for in vitro replication of adenovirus DNA complexed with viral basic proteins"Journal of Molecular Biology. 311. 41-55 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mitsuru Okuwaki: "Function of a mucleophosmin/B23, a mucleolar acidic protein, as a histone chaperone"FEBS Letters. 506. 272-276 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fumitaka Momose: "The cellular splicing factor RAF-2p48/NPI-5/BAT1/UAP56 interacts with the influeazs virus aucleoprotein and enhances viral RNA synthesis"Journal of Virology. 75. 1899-1908 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kyousuke Nagata: "Chromatin remodeling and assembly by acidic molecular chaperones"RIKEN Review. 41. 10-11 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 百瀬文隆: "インフルエンザウイルス複製におけるウイルス因子と宿主因子の相互作用"細胞工学. 19. 51-57 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mitsuru Okuwaki, Akihiko Iwamatsu, Masafumi Tsujimoto, Kyosuke Nagata: "Identification of nucleophosmin/B23, an acidic nucleolar protein, as a stimulatory factor for in vitro replication of adenovirus DNA complexed with viral basic proteins"Journal of Molectllar Biology. 311. 41-55 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mitsuru Okuwaki, Ken Matsumoto, Masafumi Tsujimotb, Kyosuke Nagata: "Function of nucleophosmin/B23, a nucleolar acidic protein, as a histone chaperone"FEBS Letters. 506. 272-276 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fumitaka Momose, Chris F. Easier, Robert E., O.Neill, Akihiko Iwamatsu, Peter Palese, Kyosuke Nagata: "The cellular splicing factor RAF-2p48/NPI5/ BAT1/ UAP56 interacts with the influenza virus nucleoprotein and enhances viral RNA synthesis"Journal of Virology. 75. 1899-1908 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kyosuke Nagata: "Chromatin remodeling and assembly by acidic molecular chaperones"RIKEN Review. 41. 10-11 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mitsuru Okuwaki: "Identification of nucleophosmin/B23, an acidic nucleolar protein, as a stimulatory factor for in vitro replication of adenovirus DNA complexed with viral basic proteins"Journal of Molecular Biology. 311. 41-55 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mitsuru Okuwaki: "Function of nucleophosmin/B23, a nucleolar acidic protein, as a histone chaperone"FEBS Letters. 506. 272-276 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Fumitaka Momose: "The cellular splicing factor RAF-2p48/NPI-5/BAT1/UAP56 interacts with the influenza virus nucleoprotein and enhances viral RNA synthesis"Journal of Virology. 75. 1899-1908 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kyosuke Nagata: "Chromatin remodeling and assembly by acidic molecular chaperones"RIKEN Review. 41. 10-11 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Pepin,Kim: "Molecular cloning of horse Hsp90 cDNA and its comparative analysis with other vertebrate Hsp90 sequences"J.Vet.Med.Sci.. 63 . 115-124 (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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