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Systematic analysis of the neural circuits connecting the lower-order sensory neuropils and higher-order integrative regions of the Drosophila brain

Research Project

Project/Area Number 12480205
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionThe University of Tokyo (2002)
Okazaki National Research Institutes (2000-2001)

Principal Investigator

ITO Kei  The University of Tokyo, Institute of Molecular and Cellular Biosciences, Associate Professor, 分子細胞生物学研究所, 助教授 (00311192)

Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥10,600,000 (Direct Cost: ¥10,600,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2001: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2000: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsDrosophila / insect / brain / neural circuit / visual system / GAL4 enhancer trap / information pathway / projection neuron / キイロショウジョウバエ / 神経回路網 / エンハンサートラップ / 電子画像データベース / 三次元再構成 / 感覚情報 / GFP / エンハンサートラツプ
Research Abstract

In insects, visual information is processed in the optic lobe and conveyed to the central brain. Although neural circuits within the optic lobe have been studied extensively, relatively little is known about the connection between the optic lobe and the central brain. To understand how visual information is read by the neurons of the central brain, and what kind of centrifugal neurons send the control signal from the central brain to the optic lobe, we performed a systematic analysis of the visual projection neurons that connect the optic lobe and the central brain of Drosophila melanogaster. By screening about 4,000 GAL4 enhancer-trap strains, we identified 44 pathways. The overall morphology and the direction of information of each pathway were investigated by expressing cytoplasmic and presynapsis-targeted fluorescent reporters. A canonical nomenclature system was introduced to describe the area of projection in the central brain. We especially concentrated on the 14 visual projection neurons arising specifically from the lobula. Eight pathways form columnar arborization in the lobula, whereas the remaining six form tangential or tree-like arborization. Eleven are centripetal pathways, among which nine terminate in the ventrolateral protocerebrum. Terminals of each columnar pathway form glomerulus-like structure in different areas of the ventrolateral protocerebrum. The posterior lateral protocerebrum and the optic tubercle were each contributed by a single centripetal pathway. Another pathway connects the lobula on each side of the brain. Two centrifugal pathways convey signals from the posterior lateral protocerebrum to the lobula.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (12 results)

All 2006 2004 2003 2002 Other

All Journal Article (7 results) Publications (5 results)

  • [Journal Article] Systematic Analysis of the Visual Projection Neurons of Drosophila melanogaster - I : Lobula-specific Pathways.2006

    • Author(s)
      Otsuna, H., Ito, K.
    • Journal Title

      J Comp Neurol (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Integration of chemosensory pathways in the Drosphila second-order olfactory centers.2006

    • Author(s)
      Tanaka, N.K., Awasaki, T., Shimada, T., Ito, K.
    • Journal Title

      J Comp Neurol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Integration of chemosensory pathways in the Drosophila second-order olfactory centers.2004

    • Author(s)
      Tanaka, N.K., Awasaki, T., Shimada, T., Ito, K.
    • Journal Title

      Current Biology 14

      Pages: 14-14

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Integration of chemosensory pathways in the drosophila second-order olfactory centers.2004

    • Author(s)
      Tanaka, N.K., Awasaki, T., Shimada, T., Ito, K.
    • Journal Title

      Current Biology 14

      Pages: 449-457

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Cautionary observations on preparing and interpreting brain images using molecular biology-based staining techniques.2003

    • Author(s)
      Ito, K., Okada, R., Tanaka, N.K., Awasaki, ] T.
    • Journal Title

      Microsc Res Tech 62

      Pages: 170-186

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Cautionary observations on preparing and interpreting brain images using molecular biology-based staining techniques.2003

    • Author(s)
      Ito, K., Okada, R., Tanaka, N.K., Awasaki, T.
    • Journal Title

      Microsc Res Tech 62

      Pages: 170-186

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] GETDB, a database compiling expression patterns and molecular locations of a collection of Gal4 enhancer traps.2002

    • Author(s)
      Hayashi, S., Ito, K., Sado, Y., Taniguchi, M., Akimoto, A., Takeuchi, H., Aigaki, T., Matsuzaki, F., Nakagoshi, H., Tanimura, T., Ueda, R., Uemura, T., Yoshihara, M., Goto, S.
    • Journal Title

      Genesis 34

      Pages: 58-61

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kido, A., Ito, K.: "Mushroom bodies are not required for courtship behavior by normal and sexually mosaic Drosophila"Journal of Neurobiology. 52. 302-311 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hayashi, S., Ito, K., Sado, Y., Taniguchi, M., Akimoto, A., Takeuchi, H., Aigaki, T., Matsuzaki, F., Nakagoshi, H., Tanimura, T., Ueda, R., Uemura, T., Yoshihara, M., Goto, S.: "GETDB, a database compiling expression patterns and molecular locations of a collection of Gal4 enhancer traps"Genesis. 34. 58-61 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ito, K., Okada, R., Tanaka, N.K., Awasaki, T.: "Cautionary observations on preparing and interpreting brain images using molecular biology-based staining techniques"Microscopy Research & Technique. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kurusu M, Awasaki T, Masuda-Nakagawa LM, Kawauchi H, Ito K, Furukubo-Tokunaga K.: "Embryonic and larval deVelopment of the Drosophila mushroom bodies : concentric layer subdivisions and the role of fasciclin II."Development. 129. 409-419 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Verkhusha, V.V., Otsuna, H., Awasaki, T., Oda, H., Tsukita, S., Ito, K.: "An enhanced mutant of red-fluorescent protein DsRed for double labeling and developmental timer of neural fiber bundle formation"J. Biol. Chem.. 276(32). 29621-29624 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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