• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Regulation of meiotic chromosome segregation by cohesin Rec8

Research Project

Project/Area Number 12480207
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionThe University of Tokyo

Principal Investigator

WATANABE Yoshinori  Univ. of Tokyo, Grad. Sch. of Science, Associate Professor, 大学院・理学系研究科, 助教授 (20212326)

Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥16,500,000 (Direct Cost: ¥16,500,000)
Fiscal Year 2001: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2000: ¥11,800,000 (Direct Cost: ¥11,800,000)
Keywordschromosome cohesion factor / centromere / heterochromatin / HP1 / Swi6 / equational division / Rec8 / Rad21 / 還元分裂 / 減数分裂
Research Abstract

Fission yeast centromeres, like that of higher eukaryotes, are comprised of repeated DNA structures and associated heterochromatin protein complexes, that play a critical role in faithful segregation of chromosomes during cell division. Cohesin protein complexes, which are essential for sister chromatid cohesion and proper chromosome segregation, are enriched at centromeric repeats. We have identified functional and physical link, between heterochromatin and cohesin. We find that the preferential localization of cohesins at the centromeric repeats is dependent on Swi6, a conserved heterochromatin protein that is required for proper kinetochore, function. Cohesin is also enriched at the mating type heterochromatic region in a Swi6 dependent manner and is required to preserve genomic integrity of this locus. We provide evidence that a cohesin subunit Psc3 interacts with Swi6 and its mouse homolog HP1. These data define a conserved function of Swi6/HP1 in recruitment of cohesin to heterochromatic regions, promoting proper segregation of chromosomes.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Kitamura, K, Katayama, S., Dhut, S., Sato, M., Watanabe, Y., Yamamoto, M., Toda, T.: "Phosphorylation of Mei2 and Ste11 by Pat1 Kinase Inhibits Sexual Differentiation via Ubiquitin Proteolysis and 14-3-3 Protein in Fission Yeast"Dev. Cell. 1. 389-399 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sato, M., Shinozaki-Yabana, S., Yamashita A., Watanabe, Y., Yamamoto, M.: "The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling"FEBS Lett.. 499. 251-255 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mizuno Ki, K., Hasemi, T., Ubukata, T., Yamada., T., Lehmann, E., Kohli, J., Watanabe., Y., Iino, Y., Yamamoto, M., Fox, M.E., Smith, G.R., Murofushi, H., Shibata, T., Ohta, K.: "Counteracting regulation of chromatin remodeling at a fission yeast cAMP responsive element-related recombination hotspot by stress-activated protein kinase, cAMP-dependent kinase and meiosis regulators"Genetics. 159. 1467-1478 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Higuchi, T., Watanabe, Y., Yamamoto, M.: "Protein kinase A regulates sexual development and gluconeogenesis through phosphorylation of a Zn-finger transcriptional activator Rst2p in fission yeast"Mol. Cell. Biol.. 22. 1-11 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sato, M., Watanabe, Y., Akiyoshi, Y., Yarnamoto, M.: "14-3-3 protein interferes with the binding of RNA to the phosphorylated form of fission yeast meiotic regulator Mei2n"Curr. Biol.. 12. 141-145 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nonaka, N., Kitajima, T, Yokobayashi, S., Xiao, G., Yamamoto, M., Grewal, S., Watanabe, Y.: "Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast"Nature Cell Biol.. 4. 89-93 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kitamura, K., Katayama, S., Dhut, S., Sato, M., Watanabe, Y., Yamamoto, M., and Toda, T.: "Phosphorylation of Mei2 and Ste11 by Pat1 Kinase Inhibits Sexual Differentiation via Ubiquitin Proteolysis and 14-3-3 Protein in Fission Yeast"Dev. Cell. 1. 389-399 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sato, M., Shinozaki-Yabana, S., Yamashita, A., Watanabe. Y., Yamamoto, M.: "The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling"FEBS Lett. 499. 251-255 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mizuno Ki, K., Hasemi, T., Ubukata, T., Yamada, T., Lehmann, E., Kohli, J., Watanabe, Y., Iino, Y., Yamamoto, M., Fox, M. E., Smith, G. R., Murofushi, H., Shibata, T., and Ohta, K.: "Counteracting regulation of chromatin remodeling at a fission yeast cAMP responsive element-related recombination hotspot by stress-activated protein kinase, cAMP-dependent kinase and meiosis regulators"Genetics. 159. 1467-1468 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Higuchi, T., Watanahe, Y., Yamamoto, M.: "Protein kinase A regulates sexual development and gluconeogenesis through phosphorylation of a Zn-finger transcriptional activator Rst2p in fission yeast"Mol. Cell. Biol.. 22. 1-11 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sato, M., Watanabe, Y., Akiyoshi, Y., and Yamamoto, M.: "14-3-3 protein interferes with the binding of RNA to the phosphorylated form of fission yeast meiotic regulator Mei2p"Curr. Biol.. 12. 141-145 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nonaka, N., Kitajima, T., Yokobayashi, S., Xiao, G., Yamamoto, M., Grewal S., and Watanabe, Y.: "Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast"Nature Cell Biol.. 4. 89-93 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kitanura, K., Katayama, S., Dhut, S., Sato, M., Watanabe, Y., Yamamoto, M., Toda, T.: "Phosphorylation of Mei2 and Stell by Pat1 Kinase Inhibits Sexual Differentiation via Ubiquitin Proteolysis and 14-3-3 Protein in Fission Yeast"Dev.Cell. 1. 389-399 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sato, M., Shinozaki-Yabana, S., Yamashita, A., Watanabe, Y., Yamamoto, M.: "The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling"FEBS Lett.. 499. 251-255 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mizuno Ki.K., Hasemi, T., Ubukata, T., Yamada, T., Lehmann, E., Kohli, J., Watanabe, Y., et al.: "Counteracting regulation of chromatin remodeling at a fission yeast cAMP responsive element-related recombination hotspot by stress-activated protein kinase, cAMP-dependent kinase and meiosis regulators"Genetics. 159. 1467-1478 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Higuchi, T., Watanabe, Y., Yamamoto, M.: "Protein kinase A regulates sexual development and gluconeogenesis through phosphorylation of a Zn-finger transcriptional activator Rst2p in fission yeast"Mol.Cell.Biol.. 22. 1-11 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sato, M., Watanabe, Y., Akiyoshi, Y., Yamamoto, M.: "14-3-3 protein interferes with the binding of RNA to the phosphorylated form of fission yeast meiotic regulator Mei2p"Curr.Biol.. 12. 141-145 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nonaka, N., Kitajima, T., Yokobayashi, S., Xiao, G., Yamamoto, M., Grewal, S, Watanabe, Y.: "Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast"Nature Cell Biol.. 4. 89-93 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Fujishiro,M.: "MKK6/3 and p38 MAPK Pathway Activation is not Necessary for Insulin-Induced Glucose Uptake, but Regulates Glucose Transporter Expression"J.Biol.Chem.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ura,S.: "MST1-JNK promotes apoptosis via caspase-dependent and-independent pathways"Genes to Cells. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 後藤由季子: "細胞死の分子メカニズム-多様性への理解-"Molecular Medicine. 37. 384-390 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 鶴田文憲: "生存シグナルとアポトーシスシグナルのクロストーク"実験医学. 18. 1384-1390 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 浦誠司: "PI3K-Akt経路のシグナル伝達"現代科学. 増刊37. 95-103 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 森靖典: "サバイバルシグナルとアポトーシス-生のシグナル:Aktを中心に-"Apoptosis Watch for Cancer Chemotherapy. 3. 14-15 (2000)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi