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Analysis of the molecular organization of nuclear pore complexes using nuclear transport factor, importin β

Research Project

Project/Area Number 12480215
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionOsaka University

Principal Investigator

YONEDA Yoshihiro  Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (80191667)

Co-Investigator(Kenkyū-buntansha) IMAMOTO Naoko  National Institute of Genetics, Associate Professor, 構造遺伝学研究センター, 助教授 (20202145)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥15,900,000 (Direct Cost: ¥15,900,000)
Fiscal Year 2001: ¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 2000: ¥8,800,000 (Direct Cost: ¥8,800,000)
Keywordsimportin β / nuclear protein transport / nuclear pore complex / X-ray crystallography / nucleoporin / importin β / importin α / Ran
Research Abstract

In eukaryotic cells, nucleo-cytoplasmic transport of molecules is crucial for intracellular signal transduction. However, it remains unknown how proteins traverse nuclear pores directionally. In this study, in order to elucidate the molecular organization of nuclear pore complexes (NPCs), we focused on importin β, which is able to translocate through the nuclear pores in both directions by itself. The structure of the uncomplexed form of the N-terminal fragment of importin β has been solved by X-ray crystallography. Based on this structural analysis, we constructed mutant importin β proteins, in which amino acid residues protruding from molecular surface of the convex side of helices (helix A) or the concave side of helices (helix B) of the pore-binding domain are substituted with alanines. When microinjected into the cytoplasm or the nucleus of cultured cells, recombinant 4B5B mutant proteins, in which the helices B4 and B5 are mutated, significantly accumulated in the nucleus compared with the wild type. In contrast, the nuclear accumulation of recombinant 5A6A mutant proteins, in which the helices A5 and A6 are mutated, dramatically decreased. The same results were obtained by using a permeabilized cell in vitro transport assay. In addition, we found that the ability of 5A6A mutants to import the NLS-substrates into the nucleus decreased, which means that the transport ability of importin β is parallel to its own migration activity. In the future, we will be able to identify key NPC components (nucleoporins) for directional movement of proteins through nuclear pores by analyzing the affinity of mutant importin β proteins with each nucleoporin.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (83 results)

All Other

All Publications (83 results)

  • [Publications] Haraguchi, T.: "Live fluorescence imaging reveals early recruitment of emerin, LBR, HPlb and RanBP2 to reforming functional nuclear envelope"J. Cell Sci.. 113. 779-794 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsubae, M.: "Characterization of the nuclear transport of a novel leucine-rich acidic nuclear Protein-like protein"FEBS Lett.. 468. 171-175 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tachibana, T.: "Recycling of importin a from the nucleus is suppressed by loss of RCC1 function in living mammalian cells"Cell Structl funct.. 25. 115-123 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikuta, T.: "Nucleocytoplasmic shuttling of the aryl hydrocarbon receptor"J. Biochem. 127. 503-509 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanabe, K.: "The small GTP-binding protein TC10 promotes growth cone formation and nerve elongation in meuronal cells, and its transcription is induced during nerve regeneration in rat"J. Neurosci.. 20. 4138-4144 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takeda, K.: "Characterization of human herpesvirus 7 U27 gene product and identification of its nuclear localization signal"Virol.. 272. 394-401 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Lee, S.J.: "The adoption of twisted structure of importin-b is essential for the protein-protein interaction required for nuclear transport"J. Mol. Biol.. 302. 251-264 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sueishi, M.: "The FHA domain of Ki-67 antigen interacts with a novel kinesin-like protein Hklp2"J. Biol. Chem.. 275. 28888-28892 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoneda, Y.: "Nucleocytoplasmic protein traffic and its significance to cell function"Genes Cells. 5. 777-787 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoneda, Y.: "New steps toward the nucleocytoplasmic traffic of macromolecules"Cell Structl Funct.. 25. 205-206 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimizu, S.: "Essential role of voltage-dependent anion channel in various forms of apoptosis in mammalian cells"J. Cell Biol.. 152. 237-250 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagoshi, E.: "Dimerization of the sterol regulatory element-binding protein-2 via the elix-loop-helix-leucine zipper domain is prerequisite for its nuclear localization mediated by importin β"Mol. Cell. Biol.. 21. 2779-2789 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jiang, C.-J.: "Molecular cloning of a novel importin a homologue from rice, by which constitutive photomorphogenic 1 (COPl) nuclear localization signal (NLS)-protein is preferentially nuclear imported"J. Biol. Chem.. 276. 9322-9329 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akakura, S.: "A role for Hsc70 in rehulating nucleo-cytoplasmic transport of a temperature-sensitive p53 (p53Vall35)"J. Biol. Chem.. 276. 14649-14657 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kurisaki, A.: "Transforming growth factor-b induces nuclear import of Smad3 in an importin-b1 and Ran-dependent manner"Mol.Biol. Cell. 12. 1079-1091 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hieda, M.: "Nuclear import of the UlA splicesome protein is mediated by importin a/b and Ran in living mammalian cells"J. Biol. Chem.. 276. 16824-16832 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takagi, M.: "A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ku067 antigen in mitosis"J. Biol. Chem.. 276. 25386-25391 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Haraguchi, T.: "BAF is required for emerin assembly into the reforming nuclear envelope"J. Cell Sci.. 114. 4575-4585 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kobayashi, M.: "Muclear localization of duplin, a b-catenin-binding protein, is essential for its inhibitory activity on the Wnt signaling pathway"J. Biol. Chem.. 277. 5816-5822 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shibata, S.: "Nucleocytoplasmic transport of proteins and poly(A) +RNA at the reconstituted Tpr-less nuclei in living mammalian cells"Genes Cells. 7. 421-434 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katahira, J.: "Complex formation between Tap and p15 affects binding to FG-repeat nucleoporins and nucleocytoplasmic shuttling"J. Biol. Chem.. 277. 9242-9246 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyamoto, Y.: "Genomic organization and chromosomal localization of the importin al gene in the mouse"Gene. 288. 49-56 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsuoka, Yu.: "A chromodomain-containing nuclear protein, MRG15 is expressed as a novel type of dendritic mRNA in neurons"Neurosci. Res.. 42. 299-308 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsuoka, Yo.: "Bovine liver chromatin fraction contains actin polymerization activity inducing micronuclei formation when injected into prometaphase cultured cells"Cell Struct. Funct.. 27. 39-45 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] kondo, Y.: "Molecular cloning of one isotype of human lamina-associated polypeptide 1s and a topological analysis using its deletion mutants"Biochem. Biophys. Res. Commun.. 294. 770-778 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsuoka, Yo.: "Identification of Ewing's sarcoma gene product as a glycoprotcin using a monoclonal antibody which recognizes an immumodeterminant containing O-linked N-acetylglucosamine moiety"Hybridoma and Hybridomics. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sekimoto, T.: "Nuclear import and export signals"Nuclear Import and Export in Plants and Animals. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Haraguchi, T.: "Live fluorescence imaging reveals early recruitment of emerin, LBR, HP1b and RanBP2 to reforming functional nuclear envelope"J. Cell Sci.. 113. 779-794 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsubae, M.: "Characterization of the nuclear transport of a novel leucine-rich acidic nuclear protein-like protein"FEBS Lett.. 468. 171-175 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tachibana, T.: "Recycling of importin a form the nucleus is suppressed by loss of RCCI function in living mammalian cells"Cell Struct. Funct.. 25. 115-123 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikuta, T.: "Nucleocytoplasmic shuttling of the aryl hydrocarbon receptor"J. Biochcem.. 127. 503-509 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanabe, K.: "The small GTP-binding protein TC10 promotes growth cone formation and nerve elongation in neuronal cells, and its transcription is induced during nerve regeneration in rat"J. Neurosci.. 20. 4138-4144 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takeda, K.: "Characterization of human herpesvirus 7 U27 gene product and identification of its nuclear localization signal"Virol.. 272. 394-401 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Lee, S. J.: "The adoption of a twisted structure of importin-b is essential for the protein-protein interaction required for nuclear transport"J. Mol. Biol.. 302. 251-264 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sueishi, M.: "The FHA domain of Ki-67 antigen interacts with a novel kinesin-like protein Hk1p2"J. Biol. Chem.. 275. 28888-28892 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoneda, Y.: "Nucleocytoplasmic protein traffic and its significance to cell function"Genes Cells. 5. 777-787 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoneda, Y.: "New steps toward the nucleocytoplasmic traffic of macromolecules"Cell Struct. Funct.. 25. 205-206 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimizu, S.: "Essential role of voltage-dependent anion channel in various forms of apoptosis in mammalian cells"J. Cell Biol.. 152. 237-250 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagoshi, E.: "Dimerization of the sterol regulatory element-binding protein-2 via the elix-loop-helix-leucine zipper domain is prerequisite for its nuclear localization mediated by importin β"Mol. Cell. Biol.. 21. 2779-2789 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jiang, C.-J.: "Molecular cloning of a novel importin a homologue from rice, by which constitutive photomorphogenic 1 (COPI) nuclear localization signal (NLS)-protein is preferentially nuclear imported"J. Biol. Chem.. 276. 9322-9329 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akakura, S.: "A role for Hsc70 in regulating nucleo-cytoplasmic transport of a temperature-sensitive p53 (p53Val135)"J. Biol. Chem.. 276. 14649-14657 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kurisaki, A.: "Transforming growth factor-b induces nuclear import of Smad3 in an importin-b1 and Ran-dependent manner"Mol. Biol. Cell. 12. 1079-1091 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hieda, M.: "Nuclear import of the UIA splicesome protein is mediated by importin a/b and Ran in living mammalian cells"J. Biol. Chem.. 276. 16824-16832 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takagi, M.: "A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in mitosis"J. Biol. Chem.. 276. 25386-25391 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Haraguchi, T.: "BAF is required for emerin assembly into the reforming nuclear envelope"J. Cell Sci.. 114. 4575-4585 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kobayashi, M.: "Nuclear localization of duplin, a b-catenin-binding protein, is essential for its inhibitory activity on the WnO signaling pathway"J. Biol. Chem.. 277. 5816-5822 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shibata, S.: "Nucleocytoplasmic transport of proteins and poly(A)+RNA at the reconstituted Tpr-less nuclei in living mammalian cells"Genes Cells. 7. 421-434 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katahira, J.: "Complex formation between Tap and p15 affects binding to FG-repeat nucleoporins and nucleocytoplasmic shuttling"J. Biol. Chem.. 277. 9242-9246 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyamoto, Y.: "Genomic organization and chromosomal localization of the importin al gene in the mouse"Gene. 288. 49-56 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsuoka, Yu.: "A chromodomain-containing nuclear protein, HRG15 is expressed as a novel type of dendritic mRNA in neurons"Neurosci. Res.. 42. 299-308 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsuoka, Yo.: "Bovine liver chromatin fraction contains actin polymerization activity inducing micronuclei formation when injected into prometaphase cultured cells"Cell Struct. Funct.. 27. 39-45 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kondo, Y.: "Molecular cloning of one isotype of human lamina-associated polypeptide ls and a topological analysis using its deletion mutants"Biochem. Biophys. Res. Commun.. 294. 770-778 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsuoka, Yo.: "Identification of Ewing's sarcoma gene product as a glycoprotein using a monoclonal antibody which recognizes an immunodeterminant containing O-linked N-acetylglucosamine moiety"Hybridoma and Hybridomics. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sekimoto, T.: "Nuclear import and export signals"Nuclear Import and Export in Plants. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimizu, S.: "Essential role of voltage-dependent anion channel in various forms of apoptosis in mammalian cells"J. Cell Biol.. 152. 237-250 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nagoshi, E.: "Dimerization of the sterol regulatory element-binding protein-2 via the helix-loop-helix-leucine zipper domain is prerequisite for its nuclear localization mediated by importin β"Mol. Cell. Biol.. 21. 2779-2789 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Jiang, C.-J.: "Molecular cloning of a novel importin α homologue from rice, by which constitutive photomorphogenic 1 (COP1) nuclear localization signal (NLS)-protein is preferentially nuclear imported"J. Biol. Chem.. 276. 9322-9329 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akakura, S.: "A role for Hsc70 in regulating nucleo-cytoplasmic transport of a temperature-sensitive p53 (p53Val135)"J. Biol. Chem.. 276. 14649-14657 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kurisaki, A.: "Transforming growth factor-b induces nuclear import of Smad3 in an importin-b1 and Ran-dependent manner"Mol. Biol. Cell. 12. 1079-1091 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hieda, M.: "Nuclear import of the U1A splicesome protein is mediated by importin α/β and Ran in living mammalian cells"J. Biol. Chem.. 276. 16824-16832 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takagi, M.: "A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in mitosis"J. Biol. Chem.. 276. 25386-25391 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Haraguchi, T.: "BAF is required for emerin assembly into the reforming nuclear envelope"J. Cell Sci.. 114. 4575-4585 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kobayashi, M.: "Nuclear localization of duplin, a β-catenin-binding protein, is essential for its inhibitory activity on the Wnt signaling pathway"J. Biol. Chem.. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shibata, S.: "Nucleocytoplasmic transport of proteins and poly(A)+RNA at the reconstituted Tpr-less nuclei in living mammalian cells"Genes Cells. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Katahira, J.: "Complex formation between Tap and p15 affects binding to FG-repeat nucleoporins and nucleocytoplasmic shuttling"J. Biol. Chem.. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miyamoto, Y.: "Genomic organization and chromosomal localization of the importin al gene in the mouse"Gene. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsuoka, Yu.: "A chromodomain-containing nuclear protein, MRG15 is expressed as a novel type of dendritic mRNA in neurons"Neurosci. Res.. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsuoka, Yo.: "Bovine liver chromatin fraction contains actin polymerization activity inducing micronuclei formation when injected into prometaphase cultured cells"Cell Struct. Funct.. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Haraguchi,T.: "Live fluorescence imaging reveals early recruitment of emerin, LBR, HP1β and RanBP2 to reforming functional nuclear envelope."J.Cell Sci.. 113. 779-794 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Matsubae,M.: "Characterization of the nuclear transport of a novel leucine-rich acidic nuclear protein-like protein."FEBS Lett.. 468. 171-175 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tachibana,T.: "Recycling of importin α from the nucleus is suppressed by loss of RCC1 function in living mammalian cells."Cell Struct.Funct.. 25. 95-103 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ikuta,T.: "Nucleocytoplasmic shuttling of the aryl hydrocarbon receptor."J.Biochem.. 127. 503-509 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tanabe,K.: "The small GTP-binding protein TC10 promotes growth cone formation and nerve elongation in neuronal cells, and its transcription is induced during nerve regeneration in rat."J.Neurosci.. 20. 4138-4144 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takeda,K.: "Characterization of human herpesvirus 7 U27 gene product and identification of its nuclear localization signal."Virol.. 272. 394-401 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Lee,S.J.: "The adoption of a twisted structure of importin-β is essential for the protein-protein interaction required for nuclear transport."J.Mol.Biol.. 302. 251-264 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sueishi,M.: "The FHA domain of Ki-67 antigen interacts with a novel kinesin-like protein Hklp2."J.Biol.Chem.. 275. 28888-28892 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoneda,Y.: "Nucleocytoplasmic protein traffic and its significance to cell function."Genes Cells. 5. 777-787 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoneda,Y.: "New steps toward the nucleocytoplasmic traffic of macromolecules."Cell Struct.Funct.. 25. 241-242 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shimizu,S.: "Essential role of voltage-dependent anion channel in various forms of apoptosis in mammalian cells."J.Cell Biol.. 152. 237-250 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nagoshi,E.: "Dimerization of the sterol regulatory element-binding protein-2 via the helix-loop-helix-leucine zipper domain is prerequisite for its nuclear localization mediated by importin β."Mol.Cell.Biol.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Akakura,S.: "A role for Hsc70 in regulating nucleo-cytoplasmic transport of a temperature-sensitive p53 (p53Val135)."J.Biol.Chem.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kurisaki,A.: "Transforming growth factor-β induces nuclear import of Smad3 in an importin-β1 and Ran-dependent manner."Mol.Biol.Cell. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hieda,M.: "Nuclear import of the U1A splicesome protein is mediated by importin α/β and Ran in living mammalian cells."J.Biol.Chem.. (in press). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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