| Project/Area Number |
12480218
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Tokyo Dental and Medical University |
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Principal Investigator |
SHIBUUYA Hiroshi Tokyo Dental and Medical University, Professor, 難治疾患研究所, 教授 (30261324)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2001: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 2000: ¥7,600,000 (Direct Cost: ¥7,600,000)
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| Keywords | TGF-b / BRAM / BIP / C. elegans / BRA-1,2 / daf / sma / RNAi / TGF-β / BRA-1, 2 / オーガナイザー形成 / Wntシグナル / Xtwn / Smad4 / LEF1 / β-catenin / 複合体形成 |
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| Research Abstract |
The TGF-b superfamily has diverse biological activities and is involved in the early devel opment of animals. We previously identified a novel family member, BMP receptor associated molecule (BRAM), which binds to the intracellular domain of BMP type IA receptor and is involved in the BMP signaling pathway.
To identify novel molecules involved in TGF-b signaling pathways, we performed yeast two-hybrid screening using BRAM as bait. From a Xenopus cDNA library, we cloned a cDNA encoding 693 amino acids and containing an oxysterol binding protein (OSBP) motif, which we designated BRAM interacting protein (BIP). We then isolated a BIP homologue from the C. elegans that encodes 733 amino acids and also contains the OSBP-like motif. Immunoprecipitation and Western blotting studies revealed that C. elegans BIP could interact with the C. elegans BRAM homologues, BRA-1 and BRA-2. C. elegans BIP was expressed in pharyngeal muscle, hypodermis and several neuronal cells, an expression pattern overlaps with those of BRA-1 and BRA-2. Finally, we found that inhibition of BIP expression in C. elegans by double stranded RNA interference produces a Sma phenotype.
BIP was isolated using the yeast two-hybrid systems. BIP may function in TGF-b pathway and regulate body length in C. elegans.
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