| Project/Area Number |
12480225
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
KIMURA Hisoshi Shiga University of Medical Science, Molecular Neuroscience Research Center, Professor, 分子神経科学研究センター, 教授 (40079736)
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| Co-Investigator(Kenkyū-buntansha) |
AIMI Yosinari Molecular Neuroscience Research Center, Research Associates, 分子神経科学研究センター, 助手 (20231756)
YASUHARA Osamu Molecular Neuroscience Research Center, Associate Professor, 分子神経科学研究センター, 助教授 (80239772)
TOOYAMA Ikuo Molecular Neuroscience Research Center, Professor, 分子神経科学研究センター, 教授 (20207533)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2001: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2000: ¥9,800,000 (Direct Cost: ¥9,800,000)
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| Keywords | Cholinergic / Hypothalamus / Neurotransmitter / Immunohistochemistry / Colooalization / Histidine decarboxylase / Adenosine deaminase / RT-PCR / Alternative splicing / アセチルコリン / コリン神経 / 隆起乳頭体核 / スプライシング / mRNA / 遺伝子発現 |
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| Research Abstract |
A splice variant of choline acetyltransferase mRNA has recently been identified in rat pterygopalatine ganglion. An antiserum against this variant protein (pChAT) stained peripheral cholinergic neurons. In the present study, we examined the immunohistochemical localization of pChAT in rat brain. Although the antiserum failed to stain known cholinergic neurons in the brain, magnocellular neurons in the tuberomammillary nucleus (TMN) of the posterior hypothalamus were stained with the antiserum. RT- PCR experiments confirmed the expression of pChAT mRNA in rat posterior hypothalamus. The distribution pattern of pChAT-positive neurons in the nucleus was similar to that positive for histidine decarboxylase (HDC) and adenosine deaminase (ADA). Double immunohistochemical staining in rats pretreated with colchicine indicated mat virtually all pChAT-positive cells contained ADA. Some pChAT-positive cells in the nucleus were retrogradely labeled following the injection of cholera-toxin B subunit into the cerebral cortex. These results suggest the existence of a presumed corticipetal cholinergic pathway ascending from the TMN.
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