| Project/Area Number |
12480239
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Osaka University |
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Principal Investigator |
MURAKAMI Fujio Osaka University, Graduate School of Engineering Science, Professor, 大学院・基礎工学研究科, 教授 (20089882)
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| Co-Investigator(Kenkyū-buntansha) |
TAMADA Atsushi Okazaki National Research Institutes, National Institute for Basic Biology, Research Associate, 基礎生物学研究所, 助手 (60270576)
KOBAYASHI Hiroaki Osaka University, Graduate School of Engineering Science, Research Associate, 大学院・基礎工学研究科, 助手 (20314396)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥10,700,000 (Direct Cost: ¥10,700,000)
Fiscal Year 2001: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2000: ¥6,600,000 (Direct Cost: ¥6,600,000)
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| Keywords | central nervous system / neuronal cell / hindbrain / rhombic lip / cell migration / floor plate / flat whole-mount preparation / slice preparation |
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| Research Abstract |
The neurons of the precerebellar nuclei, which include the pontine nuclei (PN), the external cuneatus nucleus (ECN), the lateral reticular nucleus (LRN), and the inferior olivary nucleus (ION), are all generated from lower rhombic lips (dorsal rims of the medulla) at different periods. They then migrate tangentially and finally stop their proper positions ipsilaterally (PN and ION) or contralaterally (ECN and LRN) to the ventral midline, floor plate (FP). The features of their migrations raise some questions about how they migrate tangentially ; why do some of them cross the FP, but the others not ; how do they find their correct positions. To begin to answer these questions, we have made an attempt to reconstitute the precerebellar neuronal migration in vitro. We adopted the flat-whole mount explant culture of the medulla in combination with the transplantation of lower rhombic lip from the transgenic rat that expresses GFP in all tissues to visualize the migrating neurons in in vivo-like environment. After 2 days in culture, many GFP-positive neurons with a long leading process migrating out into the host explant were observed. Most of these cells took a tangential path toward the FP as they do in vivo. Immunostaining also showed that they express TAG-1 and DCC. After 3 days, some of them crossed the FP and continued to migrate. These neurons were initially attracted by the floor plate (FP) at the ventral midline. However, after crossing the midline, they lost their responsiveness to the FP and became attracted by the alar plate (AP). These results identify a crucial change in the response of migrating cells to attractive guidance cues during the transmedian migration of precerebellar neurons.
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