| Project/Area Number |
12480250
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory animal science
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
ITOH Kikuji Graduate School of Agriculture and Life Sciences, The University of Tokyo, Assoc. Prof., 大学院・農学生命科学研究科, 助教授 (50100045)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ITOH Toshio Central Inst. of Exp. Anim., Group Director (Researcher), 実験動物中央研究所, 室長(研究職) (20106644)
NAKAYAMA Hiroyuki Graduate School of Agriculture and Life Sciences, The University of Tokyo, Assoc. Prof., 大学院・農学生命科学研究科, 助教授 (40155891)
平山 和宏 東京大学, 大学院・農学生命科学研究科, 助手 (60208858)
KOBAYASHI Kimio Central Inst. of Exp. Anim., Group Director (Researcher), 実験動物中央研究所, 室長(研究職) (20205454)
OHNISHI Yasuyuki Central Inst. of Exp. Anim., Group Director (Researcher), 実験動物中央研究所, 室長(研究職) (70201382)
|
|---|
| Project Period (FY) |
2000 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2001: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2000: ¥9,700,000 (Direct Cost: ¥9,700,000)
|
|---|
| Keywords | Cancer / ras mice / transgenic mice / c-Ha-ras / p53 |
|---|
| Research Abstract |
1. RasH2 mice harboring human prototype c-Ha-ras genes were injected with 1,2-dimethylhydrazine and examined for mutation and expression levels of human c-Ha-ras, murine Ki-ras, Na-ras and p53. There were no point mutations in these genes. The expression level of transgene mRNA determined by real time RT-PCR was shown to be higher in tumors than in non-tumor colorectal tissues, and expression levels by RT-PCR of murine Ki-ras, Na-ras and p53 in the tumors were no different from those in the non-tumor colorectal tissues.
2. Expression levels of cancer related genes in the tumors and the non-tumor tissues of rasH2 and non-Tg mice were compared using Gene Navigator^<TM> cDNA Array System (TOYOBO). For 11 genes, MMP7, Clusterin, nm23, c-jun, SLC, Bad, Bax, Gelsolin, Ran, SkpI, and p19, expression levels were higher in the tumors than in the non-tumor colorectal tissues in both rasH2 and non-Tg mice.
3. To confirm the high expression levels of 11 genes mentioned above, RT-PCR analysis was performed. Only. MMP7 and Clusterin genes were expressed more in the tumors than in the non-tumor tissues in both rasH2 and non-Tg mice.
4. Expression levels of cancer related genes between tumors and non-tumor tissues in rasH2 mice were similar to those in non-Tg mice.
|
