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Design of Artificial Cell Membrane Interface and Development of Evaluation System for Biorecognition Function

Research Project

Project/Area Number 12554029
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 分離・精製・検出法
Research InstitutionTohoku University

Principal Investigator

HAYASHITA Takashi  Tohoku University, Dept. of Chem., Associate Professor, 大学院・理学研究科, 助教授 (70183564)

Co-Investigator(Kenkyū-buntansha) NISHIZAWA Seiichi  Tohoku University, Dept. of Chem., Research Associate, 大学院・理学研究科, 助手 (40281969)
UCHIDA Tatsuya  Tohoku University, Dept. of Chem., Research Associate, 大学院・理学研究科, 助手 (30261548)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 2001: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2000: ¥10,800,000 (Direct Cost: ¥10,800,000)
KeywordsArtificial cell / Micelle / Vesicle / Thiourea probe / Crown ether probe / Anion recognition / Alkali metal ion recognition / Second harmonic generation / アルカリ金属イオン / 比色分析
Research Abstract

To develop an anion sensing system in water based on a chromoionophore/artificial cell membrane conjugate, novel thiourea-based chromoionophores having various lengths of alkyl chains (Cn-TU) were designed and their anion recognition function in aqueous vesicle solutions were evaluated. This study revealed that the positioning of chromophore binding sites inside the didodecyldimethylammonium bromide (DDAB) vesicle was successfully controlled by the alkyl chain length of Cn-TU. The cationic vesicle interface was found to exhibit a filter function for the less hydrophobic anions, and highly selective anion recognition via hydrogen bonding was achieved by C1-TU located deep inside the DDAB vesicle. Function of crown ether chromoionophores for alkali metal ion recognition in micellar system were also examined. The surface charge of the micelle was found to strongly influence the ion selectivity and sensitivity, and selective Na^+ recognition was only performed by anionic micellar system. To evaluate ion recognition at these membrane interfaces, a resonant second harmonic generation (SHG) analysis system was developed by using 15-crown-5 azophenol as a model probe. Selective sorption of alkali metal complexes with the azoprobe at heptane/water interface was confirmed by SHG spectra, and their conformation changes upon metal recognition at the interface were successfully evaluated by SHG analysis.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] T.Hayashita, T.Onodera, R.Kato, N.Teramae et al.: "Positioning Dependent Anion Recognition by Thiourea-Based Chromoionophores via Hydrogen Bonding in Vesicle Solution"Chem.Commun.. 755-756 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 山内晶世, 早下隆士: "光情報変換に基づく金属イオン認識プローブの設計と機能"Bunseki Kagaku. 49(2). 75-89 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] R.Kato, S.Nishizawa, T.Hayashita, N.Teramae: "A Thiourea-Based Chromoionophore for Selective Binding and Sensing of Acetate"Tetrahedron Letter. 42. 5053-5056 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 早下隆士, 加藤 亮, 小野寺恒信, 西沢精一, 寺前紀夫: "チオ尿素型比色プローブ/ベシクル複合体センサーの設計と機能評価"東北大学大学院理学研究科附属化学 機器分析センター年報. 1. 5-9 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Hayashita, T. Onodera, R. Kato, S. Nishizawa, N. Teramae: "Positioning Dependent Anion Recognition by Thiourea-Based Chromoionophores via Hydrogen Bonding in Vesicle Solution"Chem. Commun.. 755-756 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] A. Yamauchi, T. Hayashita: "Design and Function of Metal-ion Probes Based on Photosignal Transduction"Bunseki Kagaku. 49 (2). 75-89 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] R. Kato, S. Nishizawa, T. Hayashita, N. Teramae: "A Thiourea-Based Chromoionophore for Selective Binding and Sensing of Acetate"Tetrahedron Letter. 42. 5053-5056 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Hayashita, R. Kato, T. Onodera, S. Nishizawa, N. Teramae: "Design and Function of Thiourea Chromoionophore/Vesicle Complex Sensor"Annual Report for Instrumental Analysis Center for Chemistry, Graduate School of Science, Tohoku University. 1. 5-9 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] R.Kato, S.Nishizawa, T.Hayashita, N.Teramae: "A Thiourea-Based Chromoionophore for Selective Binding and Sensing of Acetate"Tetrahedron Letter. 42. 5053-5056 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 早下隆士, 加藤 亮, 小野寺恒信, 西沢精一, 寺前紀夫: "チオ尿素型比色プローブ/ベシクル複合体センサーの設計と機能評価"東北大学大学院理学研究科付属化学機器分析センター年報. 1. 5-9 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Hayashita,T.Onodera,S.Nishizawa,N.Teramae, et al.: "Positioning Dependent Anion Recognition by Thiourea-Based Chromoionophores via Hydrogen Bonding in Aqueous Vesicle Solution"Chemical Communications. 755-756 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] A.Yamauchi,T.Hayashita: "Design and Function of Metal Ion Probes Based on Photosignal Transduction"Bunseki Kagaku. 49. 75-89 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] A.Tong,T.Hayashita,B.D.Smith,N.Teramae, et al.: "Boronic Acid Fluorophore/β-Cyclodextrin Complex Sensors for Selective Sugar Recognition in Water"Analytical Chemistry. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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