| Project/Area Number |
12555228
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
生物・生体工学
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| Research Institution | Toyohashi University of Technology |
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Principal Investigator |
TANAKA Saburo Toyohashi University of Technology, Dep.of Engineering Professor, 工学部, 教授 (10271602)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUZAWA Yukiko Toyohashi University of Technology, Dep.of Engineering reseach Assoc., 工学部, 助手 (10273335)
KATSURA Shinji Toyohashi University of Technology, Dep.of Engineering assoc.Professor, 工学部, 助教授 (10260598)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥9,700,000 (Direct Cost: ¥9,700,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2001: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2000: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | Assay / SQUID / sensor / 交流 / 磁性微粒子 / 磁気センサ / センチネルリンパ節 / がん / ラジオガイド / ELISA / Immunoassay / Magnetic Sensor / Superconductor / 抗原抗体反応 |
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| Research Abstract |
A high sensitive analysis method for biological molecules has been requested accompanied with recent development of molecular biology. Conventional high sensitive detection methods are based on labeling techniques of fluorescent dye or enzyme. However, since those techniques involve some problems on instability of signal, a new technology is desired. Because SQUID is an extremely high sensitive magnetic sensor, it can be applied to high sensitive detection of DNA labeled with magnetic small particles. The signal from SQUID is stable in contrast to fluorescent dye and enzyme, therefore it permits long time and high sensitive measurement.
To demonstrate availability of this method, sample coverslips on which the magnetic small particles were anchored through biotin labeled DNA was prepared. Scanning of high Tc SQUID sensor on the coverslip demonstrated that magnetic flux on the coverslip agreed well to the pattern of labeled DNA anchored on the covershp. This result suggests that SQUID can be applied for specific detection of DNA molecules, especially for detection of DNA chips.
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