| Project/Area Number |
12555259
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
高分子合成
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| Research Institution | Nagoya University |
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Principal Investigator |
AOI Keigo Nagoya University Graduate School of Bioagricultural Sciences. Associate Professor, 大学院・生命農学研究科, 助教授 (30222467)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Ayami Mitsubishi-Tokyo Pharmaceuticals , Inc. . Researcher, 製薬技術開発研究センター, 研究員
OKADA Masahiko College of Bioscience and Biotechnology. Chubu University, Professor, 生物機能開発研究所, 教授 (20023103)
NOMURA Nobuyoshi Nagoya University Graduate School of Bioagricultural Sciences. Assistant Prof., 大学院・生命農学研究科, 助手 (70291408)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥5,000,000 (Direct Cost: ¥5,000,000)
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| Keywords | dendrimer / sugar ball / polypeptide / molecular recognitions / molecular capsule / carbohydrate polymer / biomedical materials / DNA |
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| Research Abstract |
The present study is concerned with synthesis of sugar/lipid-hybrid sugar balls and reading of a guest molecule into the host molecule. Galactose-bearing glycopeptide-type sugar ball capsule was designed, which was expected to interact with liver cell specifically. Polymer reaction of poly(amido amine) dendrimer with peracetylated sugar-substituted serine N carboxyanhydride (NCA) was carried out in the presence of 8-anilino-l-naphthalenesulfonate (ANS) in chloroform at -30 ゜C for 15 min. Acyl groups were introduced to the terminal amino groups to form a hydrophobic layer.Then, the product was deacetylated with hydrazine monohydrate in methanol. UV and fluorescence measurements indicated that the existence of ANS inside the sugar ball capsule.
Synthesis of "sugar cylinders", novel graft copolymers [poly(L-lysine)-graft glycopeptide-persubstituted poly(amido amine) dendrimer] was also carried out by the polymer reaction of poly(L-lysine)-graft-poly(amido amine) dendrimer with peracetylated N-acetyl-D glucosamine-substituted L-serine N-carboxyanhydride in chloroform at -30 ゜C, Functionality of the terminal amino groups in the polymer reaction was 1.0, which was determined by NMR spectro scopy.
Cell recognition ability of the sugar ball/plasmid DNA complexes was examined by erythrocyte agglutination inhibition assays using wheat germ agglutinin (WGA). The minimum inhibitory concentrations of the sugar ball/DNA complexes were well fitted with those of corresponding sugar balls. The nuclease digestion of the complex by restriction endonucleases was investigated at 37 ゜C. DNA dissociated by poly(aspartic acid) appeared resistant to the nucleases.
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