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PROPAGATION AND FIELD TRIAL OF TRANSGENIC PERSIMMON ROOTSTOCKS WITH THE GENE FOR COMPATIBLE SOLUTE BIOSYNTHESIS

Research Project

Project/Area Number 12556005
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 園芸・造園学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TAO Ryutaro  KYOTO UNIVERSITY, GRADUATE SCHOOL OF AGRICULTURE, ASSOCIATE PROFESSOR, 農学研究科, 助教授 (10211997)

Co-Investigator(Kenkyū-buntansha) KANAYAMA Yoshinori  TOHOKU UNIVERSITY, GRADUATE SCHOOL OF AGRICULTURAL SCIENCE, ASSOCIATE PROFESSOR, 農学研究科, 助教授 (10233868)
TETSUMURA Takuya  MIYAZAHI UNIVERSITY, FACULTY OF AGRICULTURE, ASSOCIATE PROFESSOR, 農学部, 助教授 (00227498)
HABU Tsuyoshi  KYOTO UNIVERSITY, GRADUATE SCHOOL OF AGRICULTURE, INSTRUCTOR, 農学研究科, 助手 (60335304)
Project Period (FY) 2000 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2003: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2000: ¥4,900,000 (Direct Cost: ¥4,900,000)
KeywordsGLYCINE BETAINE / SORBITOL / CUTTINGS / AGROBACTERIUM / ソルビトール / 挿木
Research Abstract

Many organisms, including certain plants, accumulate water-soluble organic compounds of low molecular weight that have no inhibitory effects on metabolism. These compounds are referred to as compatible solutes and allow them to tolerate certain types of environmental stress not only by adjusting osmotic pressure in cells but also by stabilizing the quaternary structures of complex proteins. Recent studies have shown that enhancement of tolerance to environmental stresses in plants can be achieved by genetic engineering of the biosynthesis of compatible solutes. All these studies, however, deal with herbaceous plant species. We selected Japanese persimmon (Diospyros kaki), which is one of the major fruit crops in Japan and susceptible to damage from salt and drought stresses, as a candidate for the first woody plant species to be genetically engineered for tolerance to environmental stress by transforming the genes involved in compatible solutes. Chimeric genes constructed from the choline oxidase gene of Arhrobacter globiformis and the cDNA for sorbitol-6-phosphate dehydrogenase of apple (Malus x domestica) were integrated into the persimmon genome to confer the ability to produce glycinebetaine and sorbitol, respectively. Tolerance to salt stress of transformed persimmon plants were determined by measuring the ratio of the variable (Fv) to the maximum (Fm) fluorescence of chlorophyll in leaves under NaCl stress. The rate of decline in Fv/Fm under NaCl stress was lower in transgenic persimmon plants producing glycinebetaine or sorbitol than control transformed and non-transformed lines. The possibility of using these transgenic persimmon as a rootstock was tested.

Report

(5 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Tao, R.: "Agrobacterium - medcated genetic transformation in Japanese Persimmon"Acta Horticulturae. 601. 57-63 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Deguchi M, M.Watanabe, Y.Kanayama: "Increase in sorbitol biosynthesis in stressed Japanese pear leaves"Acta Horticulturae. 587. 511-517 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tetsumura T, R.Tao, A.Sugiura: "Factors affecting rooting of Japanese persimmon hardwood cuttings"J.Japan.Soc.Hort.Sci. 70. 163-169 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tetsumura T, R.Tao, A.Sugiura: "Some factors affecting the rooting of softwood cuttings of Japanese persimmon"J.Japan Soc.Hort.Sci.. 70. 275-280 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tetsumura T, R.Tao, A.Sugiura: "Single node stem cuttings from root suckers to propagate a potentially dwarfing rootstock for Japanese persimmon"Hort Technology. 10. 776-780 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Gao M, R.Tao, K.Miura, A.M.Dandekar, A.Sugiura: "Transformation of Japanese persimmon with apple cDNA encoding NADP-dependent sorbitol-6-phosphate dehydrogenase"Plant Science. 160. 839-847 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Deguchi, M., M.Watanabe, Y.Kanayama: "Increase in sorbitol biosynthesis in stressed Japanese pear leaves"Acta Horticulturae. 587. 511-517 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Gao, M., A.Sakamoto, K.Miura, N.Murata, A.Sugiura, R.Tao: "Transformation of Japanese persimmon (Diospyros kaki Thunb.) with a bacterial gene for choline oxidase"Mol.Breed.. 6. 501-510 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Gao, M., R.Tao, K.Miura, A.M.Dandekar, A.Sugiura: "Transformation of Japanese persimmon (Diospyros kaki Thunb.) with apple cDNA encoding NADP-dependent sorbitol-6-phosphate dehydrogenase"Plant Science. 160. 839-847 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tao, R: "Agrobacterium-mediated genetic transformation in Japanese persimmon"Acta Hortic. 601. 57-63 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tao, R., M.Gao: "Technologies for improving tolerance to environmental stress: genetic engineering of salt stress tolerance in Japanese persimmon (Diospyros kaki) with the genes involved in biosynthesis of compatible solute"Acta Hortic. 625. 307-320 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tetsumura, T: "Cutting propagation of Diospyros rhombifolia"Acta Hortic. 517. 167-174 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tetsumura T., R.Tao, A.Sugiura: "Single-node stem cuttings from root suckers to propagate a potentially dwarfing rootstock for Japanese persimmon"Hort Technology. 10. 776-780 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Testsumura, T., R.Tao, A.Sugiura: "Factors affecting rooting of Japanese persimmon hardwood cuttings"J.Japan.Soc.Hort.Sci.. 70. 163-169 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tetsumura, T., R.Tao, A.Sugiura: "Some factors affecting the rooting of softwood cuttings of Japanese persimmon"J.Japan.Soc.Hort.Sci.. 70. 275-280 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tao, R: "Agnobacterium-mediated genetic transformation in Japanese perstmmon"Acta Horticulturae. 601. 57-63 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 出口道仁, 児下佳子, 高マイ, 鉄村琢哉, 田尾龍太郎, 金山喜則: "ソルビトール合成能を賦与したカキの倭化機構の検討"園芸学会誌. 第71巻別冊2. 511 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tetsumura, T., R.Tao, A.Sugiura: "Factors affecting rooting of Japanese persimmon hardwood cuttings"J.Japan.Soc.Hort.Sci.. 70. 163-169 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tetsumura, T., R.Tao, A.Sugiura: "Some factors affecting the rooting of softwood cuttings of Japanese persimmon"J.Japan.Soc.Hort.Sci.. 70. 275-280 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 田尾龍太郎, 高マイ, 杉浦 明: "アグロバクテリウム法によるカキの形質転換系の確率とその応用"植物の生長調節. 36. 91-96 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tetsumura,T.,R.Tao,and A.Sugiura: "Single-node stem cuttings form root suckers to propagate a potentially dwarfing rootstock for Japanese persimmon"HortTechnology. 10. 776-780 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Gao,M.,A.Sakamoto,K.Miura,N.Murata,A.Sugiura,and R.Tao: "Transformation of Japanese persimmon (Diospyros kaki Thunb.) with a bacterial gene for choline oxidase"Molecular Breeding. 6. 501-510 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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