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Bactericidal principle of the fermented aqueous extract of Stevia rebaudiana Bertoni and a possible use of the Stevia extract as a feed supplemen for prevention of the digestive tract infections.

Research Project

Project/Area Number 12556010
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 応用微生物学・応用生物化学
Research InstitutionTohoku University

Principal Investigator

KAMIO Yoshiyuki  Graduate School of Agricultural Science, Tohoku University, Professor, 大学院・農学研究科, 教授 (00109175)

Co-Investigator(Kenkyū-buntansha) ABE Naoki  Graduate School of Agricultural Science, Tohoku University, Research Associate, 大学院・農学研究科, 助手 (90202671)
KANEKO Jun  Graduate School of Agricultural Science, Tohoku University, Research Associate, 大学院・農学研究科, 助手 (30221188)
TOMITA Toshio  Graduate School of Agricultural Science, Tohoku University, Assitant Professor, 大学院・農学研究科, 助教授 (00126129)
SHIRAISHI Hiroyuki  Microbiology Division, Miyagi Prefectural Institute of Public Health and Environment, Head of Microbiology Division, 微生物部, 部長(研究職)
UGA Toshiyuki  JBB Stevia Laboratory, Researcher, 研究員(研究職)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2001: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2000: ¥8,400,000 (Direct Cost: ¥8,400,000)
KeywordsStevia rebaudiana Bertoni / bactercidal substances / fermehted extract / domestic animals / bacterial infection / feed supplement / digestive tract / growth promotion / 細菌感染
Research Abstract

We have previously shown that a fermenteds extract from the Paraguayan composite plant, Stevia rebaudiana Bertoni has strong bactericidal activity towards a wide range of food-borne pathogenic bacteria, but it shows no significant killing activity to Bifidobacteria and Lactobacilli. Here we studied bactericidal principle of the fermented extract, and tested the extract for possible use as a feed supplement for prevention of the intestinal tract infections of domestic animals and for promotion of animal growth.
1. Fermented aqueous extract of Stevia rebaudiana Bertoni was fractionated by the partition with ethyl acetate, followed by the high performance column chromatography using an ODS (C_<18>) column. Two bactericidal fractions and one enhancer fraction were obtained. Viability of E. coli was reduced from 10^9 cfu/ml to <10^3 cfu/ml when incubated with the bactericidal fractions at 37℃ for 1 h. One bactericidal fraction has the maximal absroption at 280 nm, and it was sensitive to light irradiation. The other bactericidal fraction was identified as lactic acid.
2. In the laboratory experiments, oral administration of the Stevia extract to the Salmonella-infected calves reduced viable bacteria in the intestinal tract and bacterial tissue invasion, although not prevented the lethal effect of the bacterium.
3. In the field experiments, oral administration of the Stevia extract was effective to the intestinal tract infections of piggys. Many of the animals with early-staged diarrhea were recovered within 3 days after the treatment, and their growth rate was normal threeafter. Oral adminitration of the diluted Stevia extract promoted growth of pigs. Livers of the Stervia-fed pigs were evaluated to be very healthy at the slaughter house. Taste of the pork meat was exellent.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Y.Yamamoto, M.Higuchi, L.B.Poole, Y.Kamio: "Pole of the dpr product in oxygen tolerance in Streptococcus mutans"J.Bacteriol. 3740-3747 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Nakamura, T.Tomita, N.Abe, Y.Kamio: "Purification and characterization of an extracellular poly (L-lactic acid) depolymerase from a soil isolate, Amycolatopsis sp. strain K 104-1"Appl.Envirn.Microbiol.. 67(11). 345-353 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] A.H.Nguyen, T.Tomita, J.Kaneko, T.Hayashi, Y.Kamio: "DNA inversion in the tail fiber gene alters the host range specificity of carotovoricin Er, a phage-tail-like bacteriocin of Erwinia carotovora Er"J.Bacteriol. 183(21). 6274-6281 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sawada, H., Yanagida, K., Sugiya, M., Tomita, T.: "Antibacterial activity of novel fluoroalkyl end-capped cooligomers containing dimethyl(octyl)ammonium segments"Eur.Polym.J.. 57(7). 1433-1440 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Sawada, Y.Murai, M.Kurachi, T.Kawase, T.Tomita: "Synthesis and antibacterial activity of novel fluoroalkyl end-capped oligomers containing ammonium segments"J.Materials Chem.. 12(2). 188-194 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Yamamoto, L.B.Poole, R.R.Hantgan, Y.Kamio: "An iron-binding protein, Dpr from Streptococcus mutans prevents iron-dependent hydroxyl radical formation in vitro"J.Bacteriol. (印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Yamamoto, M. Higuchi, L B. Poole, and Y. Kamio: "Role of the dpr product in oxygen tolerance in Streptocococus mutans"J. Bacteriol.. 182. 3740-3747 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Nakamura, T. Tomita, N. Abe, and Y, Kamio: "Purification and characterization of an extracellular poly (L-lactic acid) depolymerase from a soil isolate, Amycolatopsis sp. strain K 104-1"Appl. Envirn. Microbiol.. 67. 345-353 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] A. H. Nguyen, T. Tomita, J. Kaneko, T. Hayashi, Y. Kamio: "DNA inversion in the tail fiber gene alters the host range specificity of carotovoricin Er, a phage-tai-like bacteriocin of Erwinia carotovora Er."J. Babteriol.. 183. 6274-6281 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sawada, H., Yanagida, K., Sugiya, M., and Tomita, T.: "Antibacterial activity of novel fluoroalkyl end-capped cooligomers containing dimethyl(octyl)ammonium segments"Eur. Polym. J.. 57. 1433-1440 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Sawada, Y. Murai, M. Kurachi, T. Kawase, T. Tomita: "Synthesis and antibacterial activity of novel fluoroalkyl end-capped oligomers containing ammonium segments"J. Materials Chem.. 12. 188-194 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Yamamoto, L. B. Poole, R. R. Hantgan, Y. Kamio: "An iron-binding protein, Dpr from Streptococcus mutans prevents iron-dependent hydroxyl radical formation in vitro"J. Bacteriol.. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Nakamula, T.Tomita, N.Abe, Y.Kamio: "Purification and characterization of an extracellular poly (L-lactic acid) depolymerase from a soil isolate, Amycolatopsis sp. strain K IO4-1"Appl. Envirn. Microbiol. 67(1). 345-353 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Narita, J.Kaneko, J.Chiba, J.Etienne, Y.Pieont Y.Kamio: "Phage conversion of Panton-Valentine leukocidin (PVL) in Staphylococcus aureus : molecular analysis of a PVL-converting phage,φSLT,"Gene. 268. 195-206 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.H.Nguyen, T.Tomita, M.Hirota, J.Kaneko, T.Hayashi, Y.Kamio: "DNA inversion in the tail fiber gene alters the host range specificity of carotovoricin Er, a pnage-tai1-like bacteriocin of Erwinia carotovora subsp. carotovora Er,"J. Bacteriol. 183(21). 6274-6281 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Yamamoto, L.B.Poole, R.R.Hantgan, Y.Kamio: "An iron-binding Protein, Dpr from Streptococcus mutans prevents iron-dependent hydroxyl radical formation in vitro"J. Bacteriol. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Yamaguchi, Y.Takatsuka, Y.Kamio: "Identification of a 22-kDa protein required for the degradation of Selenomonas ruminantium lysine decarboxylase by ATP-dependent protease"Biosci. Biothehnol. Biochem. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Sawada, Y.Murai, M.Kurachl, T, Kawase, Y.Minami, J.Kyokane, J., T.Tomita: "Synthesis and antibacterial activity of novel fluoroalkyl end-capped oligomers containing ammonium segments : application to new fluorinated gelling materials with an antibacterial activityy"J. Materials Chem. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sawada,H.,Yanagida,K.,Inaba,Y.,Sugiya,M.,Kawase,T.,and Tomita,T.: "Antibacterial activity of novel fluoroalkyl end-capped cooligomers containing dimethyl (octyl) ammonium segments."Eur.Polym.J.. (in press.). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nguyen,A.H.,Tomita,T.,Hayashi,T.,and Kamio,Y.: "A DNA inversion in the tail fiber gene alters the host range specificity of carotovoricin Er, a phage-tail-like bacteriocin of Erwinia carotovora subsp.carotovora Er."Mol.Microbiol.. (in press.). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Higuchl,Y.Yamamoto,and Y.Kamio: "Molecular Biology of oxygen tolerance in lactic acid bacteria : Functions of NADH oxidases and Dpr in oxydative stress"Journal of Bioscience and Bioengineering. 90(5). 484-493 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Yamamoto,M.Higuchi,L.B.Poole,and Y.Kamio: "Role of the dpr product in oxygen tolerance in Streptococcus mutans"Journal of Bacteriology. 182(13). 3740-3747 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Yokota and Y.Kamio: "Tyrosine72 residue at the bottom of rim domain in LukF crucial for the sequential binding of the staphylococcal γ-hemolysin to human erythrocytes"Biosci.Biotechnol.Biochem.. 64(12). 2744-2747 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] D.Zou,J.Kaneko,S.Narita,and Y.Kamio: "Prophage φPV83-pro,carrying Panton-Valentine leukocidin genes, on the Staphylococcus aureus P83 chromosome : comparative analysis of the genome structures of φPV83-pro, φPVL, φ11, and other phages"Biosci.Biotechnol.Biochem.. 64(12). 2631-2643 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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