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Development of bioremediation processes under anoxic conditions using denitrifying bacteria

Research Project

Project/Area Number 12556014
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 応用微生物学・応用生物化学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KATO Nobuo  Graduate School of Agriculture Professor Division of Applied Life Sciences, 農学研究科, 教授 (50026556)

Co-Investigator(Kenkyū-buntansha) KIMURA Toshiaki  PF Bio-Lab. Toyota Co. Ltd. Researcher, FPバイオ・ラボ, 研究員
YURIMOTO Hiroya  Graduate School of Agriculture Assistant Division of Applied Life Sciences Professor, 農学研究科, 助手 (00283648)
SAKAI Yasuyoshi  Graduate School of Agriculture Associate Division of Applied Life Sciences Professor, 農学研究科, 助教授 (60202082)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 2002: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2001: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥6,900,000 (Direct Cost: ¥6,900,000)
KeywordsAzoarcus sp / Thauera sp / Magnetospirillum sp / denitrifying bacteria / toluene / anaerobic degradation of toluene / benzyl succinate synthase / benzoyl CoA reductase / 脱窒性細菌 / 嫌気分解 / バイオレメディエーション / Thauera sp. / Mognetospirillum sp. / Thauera sp. DNT1 / Magnetospirillum / フェノール / 芳香族化合物
Research Abstract

1) Analysis of microbial consortia for degradation of aromatic compounds under denitrifying conditions
Bacteria that degrade aromatic compounds under denitrifying conditions had been restricted strains belonging to genera Thauera and Azoarcus. In this project, we have isolated a number of novel bacterial strains that belonged to genus Magnetospirillum. The isolates were divided into 3 groups through phylogenetic analysis with 16S rDNA, which correspond to availability of aromatic compounds for growth.
2) Pathway for degradation of aromatic compounds by the isolates
(1) Toluene degradation by Thauera sp. DNT-1: The organism was found to be able to degrade toluene under both aerobic and denitrifying conditions. Toluene dioxygenase (Tod) and benzyl succinate synthase (Bss) were cloned. From the results of transcriptional analyses of the genes, Bss could be effective only under anaerobic conditions, while tod gene product functioned under aerobic conditions.
(2) Cloning of genes for toluene degradation by Magnetospirillum spp. The genes encoding main enzymes participating in toluene degradation under denitrifying conditions were cloned from Magnetospirillum sp. TS-6. From the result, the degradation pathway of the organism could be confirmed.
3) Assignment of the denitrifying bacteria under in-situ-bioremediation
Benzoyl CoA reductase was found as a key enzyme for the ring fission a variety of anaerobic toluenedegraders. We proposed a monitoring system for anaerobic aromatic-compound-degraders using benzoyl CoA reductase genes as the probe.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Y.Shinoda et al.: "Isolation and characterization of a new denitrifying spirillum capable of anaerobic degradation of phenol"Appl. Environ. Microbiol.. 66. 1286-1291 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 篠田吉史: "原位置バイオレメディエーションに有効な脱窒細菌による芳香族化合物の嫌気分解"バイオサイエンスとインダストリー. 58. 11-16 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 谷明生: "環境修復や物質生産に利用できるAcinetobacter属細菌の多様な代謝経路"バイオサイエンスとインダストリー. 59. 599-604 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Tani et al.: "Gene structure and regulation of the alkane hydroxylase complex in Acinetobacter sp. strain M-1"J. Bacteriol.. 183. 1819-1823 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Ishige et al.: "Wax ester production from n-alkanes by Acinetobacter sp. strain M-1 : Ultrastructure of cellular inclusions and role of acyl coenzyme A reductase"Appl. Envion. Microbiol.. 68. 1192-1195 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Yurimoto et al.: "The ribulose monophosphate pathway operon encoding formaldehyde fixation in a thermotolerant methylotroph, Bacillus brevis S1"FEMS Microbiol. Lett.. 214. 189-193 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Shinoda et al.: "Isolation and characterization of a new denitrifying spirillum capable of anaerobic degradation of phenol"Appl. Encrion. Microbil.. 66. 1286-1291 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Shinoda et al.: "Denitrifying bacteria useful for in situ bioremediation of aromatic compounds under anaerobic conditions"Bioscience and Industry. 58. 11-16 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A. Tani et al.: "Versatile metabolic ability of Acinetobacter spp. for bioremediation and useful compound production"Bioscience and Industry. 59. 599-602 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A. Tani et al.: "Gene structure and regulation of the alkane hydroxylase complex in Acinetobacter sp. strain M-1"J. Bacteriol.. 183. 1819-1823 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Ishige et al.: "Wax ester production from n-alkanes by Acinetobacter sp. strain M 1: Ultrastructure of cellular inclusions and role of acyl coenzyme A reductase"Appl. Envron. Microbiol. 68. 1192-1195 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Yurimoto et al.: "The ribulose monophosphate pathway operon encoding formaldehyde fixation in a thermotolerant methylotroph. Bacillus brevis S1"FEMS Microbiol. Lett.. 214. 189-193 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ishige, T: "Wax ester production by bacteria"Current Opinion Microbiol. 6・3(印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tani, A.: "Two acyl-CoA dehydrogenases of Acinetobacter sp. strain M-1 that uses very"J. Biosci. Bioeng.. 94. 326-329 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ishige, T: "Wax ester production from n-alkanes by Acinetobacter sp. M-1 : Ultrastructure of cellular inclusions and role of acyl coenzyme A reductase"Appl. Environ. Microbiol.. 68. 1192-1195 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yurimoto, H.: "The ribulose monophosphate pathway operon encoding formaldehyde fixation in a thermotolerant methylotroph Bacillus brevis S1"FEMS Microbiol. Lett.. 214. 189-193 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Lee, B.: "Physiological role of the glutathione-dependent formaldehyde dehydrogenase in the methylotrophic yeast Candida boidinii"Microbiology. 148. 2697-2704 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakagawa, T.: "Physiological role of the second alcohol oxidase gene MOD2 in the methylotrophic growth of Pichia methanolica"Yeast. 19. 1067-1073 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yurimoto, Y.: "Hansenula polymorpha Biology and Applications."Wiley-VCH. 347 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 谷明生: "環境修復や物質生産に利用できるAcinetobacter属細菌の多様な代謝機能"バイオサイエンスとインダストリー. 59. 599-604 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.Tani et al.: "Gene structure and regulation of the alkane hydroxylase complex in Acinetobacter sp. strain M-1"J. Bacteriol.. 183. 1819-1823 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.Tani et al.: "hermostable NADP(+)-dependent medium-chain alcohol dehydrogenase from Acinetobacter sp.strain M-1"Appl. Environ. Microbiol.. 66. 5231-5235 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Ishige: "one-Chain aldehyde dehydrogenese that participates in n-alkane utilization and wax ester synthesis in Acinetobacter sp.M-1"Appl. Environ. Microbiol.. 66. 3481-3486 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Shinoda et al.: "Isolation and characterization of a new denitrifying spirillum capable of anaerobic degradation of phenol."Appl.Environ.Microbiol.. 66(4). 1286-1291 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 篠田吉史 ら: "原位置バイオレメディエーションに有効な脱窒細菌による芳香族化合物の嫌気分解"バイオサイエンスとインダストリー. 58(10). 11-16 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 篠田吉史 ら: "16S rRNA遺伝子解析による細菌の系統分類法"島津評論. 57(1,2). 121-132 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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