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Development of Non-thermal Inactivation of Microorganisms and Enzyies by Use of Supercritical Carbon Dioxide

Research Project

Project/Area Number 12556021
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 食品科学・栄養科学
Research InstitutionKyushu University

Principal Investigator

SHIMODA Mitsuya  Kyushu University, Agriculture, Bioscience and Biotechnology, Asociate Prof., 大学院・農学研究院, 助教授 (70149871)

Co-Investigator(Kenkyū-buntansha) IGURA Noriyuki  Kyushu University, Agriculture, Bioscience and Biotechnology, Assistant Prof., 大学院・農学研究院, 助手 (30260722)
HAYAKAWA Isao  Kyushu University, Agriculture, Bioscience and Biotechnology, Prof., 大学院・農学研究院, 教授 (30038252)
石川 洋哉  九州大学, 大学院・農学研究院, 助手 (00325490)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 2002: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2001: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2000: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsFood Technology / Microbial inactivation / Enzyme inactivation / Non-thermal cell killing / Non-thermal enzyme inactivation / Supercriticalvcarbon dioxide / 食品加工 / 非加熱酵素失活化
Research Abstract

The effects of temperature and the concentration of dissolved CO_2 were investigated using a plug-flow system on the inactivation of S. cerevisiae. Several combinations of pressure (4, 6, 8, 10 MPa) and temperature (30, 34, 36, 38℃) were used. The inactivation followed the first-order death kinetics and the effects of the temperature and CO_2 concentration were consistent through the critical temperature and pressure of CO_2. The obtained D-values were 0.14 min at 8 MPa and 38℃, and 0.15 min at 10 MPa and 36℃. The log D-values were related linearly to the treatment temperature and to the dissolved CO_2 concentration. The value of thermal resistance constant (z_<co2>(T)) was 9.5℃ in the media including significant levels of CO_2, and the value of CO_2 resistance constant, z_<temp>(γ)=7.2γ. These results permitted estimation of the D-values at any temperature and any CO_2 concentration.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] 石川洋哉, 下田満哉, 吉村臣史: "pH-Dependent Inactivation of Enzymes by Microbubbling of Supercritical Carbon Dioxide"Food Science and Technology Research. 6. 212-215 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 石川洋哉, 下田満哉, 松本清: "Unfolding of Poly-L-glutamic Acid by Microbubbling of Supercritical Carbon Dioxide"Biosience, Biotechnology, and Biochemistry. 64. 1295-1297 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 石川洋哉, 下田満哉, 松本清: "Irresible Unfolding of Myoglobin in an Aqueous Solution by Supercritical Carbon Dioxide"Journal of Agricultural and Food Chemistry. 48. 4535-4539 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 下田満哉, J.C.-Castellanos, 早川功: "The influence of dissolved CO_2 concentration on the death kinetics of Saccharomyces serevisiae"Journal of Applied Microbiology. 91. 306-311 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 吉村臣史, 下田満哉, 早川功: "Inactivation Kinetics of Enzymes by Using Continuous Treatment with Microbubbles of Supercritical Carbon Dioxide"Journal of Food Science. 66. 694-697 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 下職満哉, 籠 寛之, 早川功: "Accelerated death kinetics of Aspergillus niger spores under high-pressure carbonation"Applied and Environmental Microbiology. 68. 4162-4167 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 下田満哉: "ミクロバブルCO_2殺菌 「食品の非加熱殺菌応用ハンドブック」"サイエンスフォーラム. 7 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroya Ishikawa, Takashi Yoshimura, Miwa Kajiwara, Mitsuya Shimoda, Kiyoshi Matsumoto, and Yutaka Osajima: "pH-Dependent Inactivation of Enzymes by Microbubbling of Supercritical Carbon Dioxide"Food Science and Technology, Research. 6. 212-215 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroya Ishikawa, Mitsuya Shimoda, Akiyoshi Yonekura, Keiko Mishima, Kiyoshi Matsumoto, and Yutaka Osajima: "Unfolding of Poly-L-glutamic Acid by Microbubbling of Supercritical Carbon Dioxide"Biosience, Biotechnology, and Biochemistry. 64. 1295-1297 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroya Ishikawa, Mitsuya Shimoda, Akiyoshi Yonekura, Keiko Mishima, Kiyashi Matsumoto, and Yutaka Osajima: "Irresible Unfolding of Myoglobin in an Aqueous Solution by Supercritical Carbon Dioxide"Journal of Agricultural and Food Chemistry. 48. 4535-4539 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mitsuya Shimoda, J. Cocunubo-Castellanos, Hiroyuki Kago, Masaki Miyake, Yutaka Osajima, and Isao Hayakawa: "The influence of dissolved CO_2 concentration on the death kinetics of Saccharomyces serevisiae"Journal of Applied Microbiology. 91. 306-311 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takashi Yoshimura, Mitsuya Shimoda, Hiroya Ishikawa, Masaki Miyake, Isao Hayakawa, Kiyashi Matsumoto, and Yutaka Osajima: "Inactivation Kinetics of Enzymes by Using Continuous Treatment with Microbubbles of Supercritical Carbon Dioxide"Journal of Food Science. 66. 694-697 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mitsuya Shimoda, Hiroyuki Kaga, Naoko Kojima, Masaki Miyake, Yutaka Osajima, and Isao Hayakawa: "Accelerated death kinetics of Aspergillus niger spores under high-pressure carbonation"Applied and Environmental Microbiology. 68. 4162-4167 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] "Applied handbook for non-thermal microbial inactivation of foods. Microbial inactivation with microbubbles of pressured CO_2"Science Forum Ltd Co.. 59-65 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mitsuya Shimoda: "Accelerated Death Kinetics of Aspergillus niger Spores under High-Pressure Carbonation"Applied and Environmental Microbiology. Vol68(8). 4162-4167 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takashi Yoshimura: "Effect of CO2 Flow Rate on Enzyme Inactivation by Continuous Method with Microbubbles of Supercritical Carbon Dioxide"J. Fac. Agr., Kyushu Univ.. Vol46(2). 345-352 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mitsuya Shimoda: "Kinetics of Microganism Death and Enzyme Inactivation in High-pressure Carbonated Solutions"Foods and Food Ingredients Journal of Japan. No197. 7-12 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 下田満哉, J.Cocunybo, 早川功: "The influence of dissolved CO_2 concentration on the death kinetics of Saccharomyces serevisiae"Journal of Applied Microbiology. 91巻・2号. 306-311 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 吉村臣史, 下田満哉, 早川功: "Inactivation kinetics of enzymes by using continuous treatment with microbubbles of supercritical carbon dioxide"Journal of Food Science. 66巻・5号. 694-697 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 下田満哉: "液状食品の二酸化炭素処理 -殺菌・酵素失活へのCO_2の利用-"食品工業. 44巻・24号. 18-24 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 下田満哉, 三宅正起, 筬島 豊: "ミクロバブル超臨界CO_2殺菌法 -殺菌効果に影響を及ぼす因子の定量化-"食晶と開発. 36巻・10号. 13-16 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 下田満哉(分担): "食品の非加熱殺菌応用ハンドブック"(株)サイエンスフォーラム. 264-7 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 石川洋哉,下田満哉,筬島豊 ら: "Irreversible Unfolding of Myoglobin in an Aqueous Solution by Spercritical Carbon Dioxide"Journal of Food and Agriculture. 48巻・10号. 4535-4539 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 石川洋哉,下田満哉,筬島豊 ら: "Unfolding of Poly-L-glutamic Acid by Microbubbling of Supercritical Carbon Dioxide"Bioscience, Biotechnology and Biochemistry. 64巻・6号. 1295-1297 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 石川洋哉,下田満哉,筬島豊 ら: "pH-Dependent Inactivation of Enzymes by Microbubbling of Supercritical Carbon Dioxide"Food Science Technology Research. 64巻・3号. 212-215 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 下田満哉: "ミクロバブル超臨界炭酸ガス殺菌"防菌防黴. 29巻・2号. 99-105 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 下田満哉,三宅正起,筬島豊 ら: "二酸化炭素を使用する液体食品の非加熱殺菌"製剤機械技術研究会誌. 9巻・4号. 9-14 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 下田満哉: "非加熱低エネルギー・リサイクル型殺菌・酵素失活システム"食品と科学. 2000・5号. 86-90 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 下田満哉(共著): "超臨界流体プロセスの実用化"(株)技術情報協会. 214(7) (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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