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Plant expression of antigens of animal pathogens and its application for oral vaccination

Research Project

Project/Area Number 12556053
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Applied veterinary science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

SUGIMOTO Chihiro  Obihiro University of Agriculture and Veterinary Medicine, National research Center for Protozoan Diseases, Professor, 原虫病研究センター, 教授 (90231373)

Co-Investigator(Kenkyū-buntansha) XUAN Xuenan  Obihiro University of Agriculture and Veterinary Medicine, National research Center for Protozoan Diseases, Associate Professor, 畜産学部, 教授 (10292096)
FUISAKI Kozo  Obihiro University of Agriculture and Veterinary Medicine, National research Center for Protozoan Diseases, Professor, 畜産学部, 教授 (00292095)
NAGASAWA Hideyuki  Obihiro University of Agriculture and Veterinary Medicine, National research Center for Protozoan Diseases, Professor, 畜産学部, 教授 (60172524)
MATSUMURA Takeshi  Advanced Institute of Science and technology, Senior Researcher, 研究員 (50358040)
UYEDA Ichiro  Graduate School of Agriculture, Hokkaido University, Professor, 大学院・農学研究所, 教授 (10113523)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 2001: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2000: ¥8,500,000 (Direct Cost: ¥8,500,000)
KeywordsPlant expression / transgenic plant / protozoa / tick / salivary gland / サイトカイン / 細胞成長因子 / 粘膜免疫 / ダニワクチン / ワクチン / 食べるワクチン / 細胞増殖因子 / TGFβ
Research Abstract

This project was aiming at expression of genes from animal pathogenic microbes, and development of mucosal vaccine as an injectable form into nasal or oral cavities in combination with cytokines. As model antigens, salivary antigens of ticks that transmit various protozoan parasite were investigated. Those antigens combined with cytokines such as interleukin-1 or -2 will be used as tick waccines.
We introduced a bovine transforming growth factor beta gene into tobacco by using Agrobacterium system, and confirmed its expression in regenerated plant. Expression was enhanced by addition of legumine signal sequence. We also expressed bovine IL-1 and IL-2 in tobacco plants. Biological activities of these cytokines were confirmed by in vitro assays.
Genes for tick cement proteins, serine proteinases, and their inhibitors (serpin) were cloned and their molecular structures were investigated. Biological activities of these molecules were also demonstrated. Vaccination of rabbit with recombinant cement proteins inhibited tick infestation, which suggested these proteins are good candidate for commercial production of tick vaccines.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] T.Hataya, K.Uchino, R.Arimoto, N.Suda, T.Sano, E.Shikata, I.Uyeda: "Molecular characterization of Hop latent virus and phylogenetic relationships among viruses closely related to carlaviruses"Arch Virol. 145. 2503-2524 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mulenga, A., Sugimoto, C., Misao Onuma, M: "Issues in tick vaccine development : identification and characterization of potentia candidate vaccine antigens"Microbes and Infection. 2. 1353-1361 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kabeya H., Fukuda A., Ohashi K., Sugimoto C., Onuma M.: "Tumor necrosis factor alpha and its receptors in experimentally bovine leukemia virus-infected sheep"Veterinary Immunology & Immunopathology. 81(1-2). 129-139 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Hataya, R.Arimoto, N.Suda, I.Uyeda: "Molecular characterization of Hop mosaic virus : its serological and molecular relationships to Hop latent virus"Arch Virol. 146. 135-1948 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohya, K., Matsumura, T., Ohashi, K., Onuma, M., Sugimoto C.: "Expression of Two Subtypes of Human Interferona in Transgenic Potatoes"J. Cytokine Interferon Res.. 21(8). 595-602 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mulenga, A., Sugimo, M., Nakajima, M., Sugimoto, C., Misao Onuma, M.: "Tick-encoded serine proteinase inhibitors (serpines) ; potential target antigen for tick vaccine development"J. Vet. Med. Sci.. 63. 1063-1069 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohya K., Itchoda N., Ohashi K., Onuma M., Sugimoto C., Matsumura T.: "Expression of biologically active human tumor necrosis factor-alpha in transgenic potato plant"Journal of Interferon & Cytokine Research. 22(3). 371-378 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kakuda T., Sugimoto C., Onuma M.: "Epitope-mapping of antigen-specific T lymphocyte in cattle immunized with recombinant major piroplasm surface protein of Theileria sergenti"Journal of Veterinary Medical Science. 63(8). 895-901 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yokoyama N, Suthisak B, Hirata H, Matsuo T, Inoue N, Sugimoto C, Igarashi I: "Cellular localization of Babesia bovis merozoite rhoptry-associated protein 1 and its erythrocyte-binding activity"Infect Immun. 70(10). 5822-5826 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yokoyama N, Bork S.Nishisaka M, Hirata H, Matsuo T, Inoue N, Xuan X, Suzuki H, Sugimoto C, Igarashi I: "Roles of the Maltese Cross Form in the Development of Parasitemia and Protection against Babesia microti Infection in Mice"Infect Immun.. 71(1). 411-417 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mulenga A, Tsuda A, Onuma M, Sugimoto C: "Four serine proteinase inhibitors (serpin) from the brown ear tick, Rhiphicephalus appendiculatus ; cDNA cloning and preliminary characterization"Insect Biochem Mol Biol.. 33(2). 267-276 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sugimoto C., Ueda, S., Masuta, C., Ohya, K., Matsumura, T., Uyeda, I.: "Development of a new plant virus vector system and expression of human interferon alfa in broad beans"Molecurar Farming (Toutant, J.P. & Balazs, E. ed. ; ISBN 2-7380-0953-0, (INRA Editions, France). 5 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsumura, T., Ohya, K., Ichouda, N., Sugimoto, C.: "Expression of human interferon alfa and tumor necrosis factor alfa in plants"Molecurar Farming (Toutant, J. P. & Balazs, E. ed. ; ISBN 2-7380-0953-0 (INRA Editions, France). 9 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohya, Matsumura, T., K., Ichouda, N., Ohashi, K., Onumna, M., Sugimoto C.: "Protective effect of orally administrated human interferon alfa against Listeria monocytogenes infection and practical advantage of HuIFN-alfa derived from transgenic potato plants"Plant Biotechnology 2002 and Beyond (I.K. Vasil, ed.)(ISBN1-4020-1126-1) : (Kluwer Academic Publishers). 3 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] C.Sugimoto, K.Fujisaki: "Non-transforming Theileria parasites in ruminants"Theileria (D. Dobbelaere ed.) (kluwer Academic Publishers). 10 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Hataya, K. Uchino, R. Arimoto, N. Suda, T. Sano, E. Shikata and I. Uyeda: "Molecular characterization of Hop latent virus and phylogenetic relationships among viruses closely related to carlaviruses"Arch. Virol. 145. 2503-2524 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mulenga, A., Sugimoto, C., Misao Onuma: "Missues in tick vaccine development: identification and characterization of potentia candidate vaccine antigens"Microbes and Infection. 2. 1353-1361 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kabeya H., Fukuda A., Ohashi K., Sugimoto C., Onuma M.: "Tumor necrosis factor alpha and its receptors in experimentally bovine leukemia virus-infected sheep"Veterinary Immunology & Immunopathology. 81(1-2). 129-139 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Hataya, R. Arimoto, N. Suda and I. Uyeda: "Molecular characterization of Hop mosaic virus: its serological and molecular relationships to Hop latent virus"Arch Virol. 146. 1935-1948 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohya, K., Matsumura, T., Ohashi K., Onuma, M., Sugimoto C.: "Expression of Two Subtypes of Human Interferona in Transgenic Potatoes, J."J. Cytokine Interferon Res.. 21(8). 595-602 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mulenga A., Sugimoto C., Ingram G., Ohashi K., Misao O.: "Characterization of two cDNAs encoding serine proteinases from the hard tick Haemaphysalis longicornis"Insect Biochemistry & Molecular Biology. 31(8). 817-825 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kakuda T., Sugimogo C., Onuma M.: "Epitope-mapping of antigen-specific T lymphocyte in cattle immunized with recombinant major piroplasm surface protein of Theileria sergenti"Journal of Veterinary Medical Science. 63(8). 895-901 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yokoyama N., Bork S., Nishisaka M., Hirata H., Matsuo T., Inoue N., Xuan X., Suzuki H., Sugimoto C., Igarashi I.: "Roles of the Maltese Cross Form in the Development of Parasitemia and Protection against Babesia microti Infection in Mice"Infect Immun.. 71(1). 411-417 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yokoyama N., Suthisak B., Hirata H., Matsuo T., Inoue N., Sugimoto C., Igarashi I.: "Cellular localization of Babesia bovis merozoite rhoptry-associated protein 1 and its erythrocyte-binding activity"Infect Immun.. 70(10). 5822-5826 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mulenga A., Sugimo, M., Nakajima, M., Sugimoto, C., Misao Onuma, M.: "Tick-encoded serine proteinase inhibitors (serpines); potential target antigen for tick vaccine development"J. Vet. Med. Sci.. 63. 1063-1069 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohya K., Itchoda N., Ohashi K., Onuma M., Sugimoto C., Matsumura T.: "Expression of biologically active human tumor necrosis factor-alpha in transgenic potato plant"Journal of Interferon & Cytokine Research. 22(3). 371-378 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sugimoto C., Ueda S., Masuta, C., Ohya, K., Matsumura, T., Uyeda I.: "Delopment of a new plant virus vector system and expression of human interferon alfa in broad beans"Molecurar Farming (Toutant, J.P. & Balazs, E. ed; ISBN 2-7380-0953-0 (INRA Editions, France). 173-177 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsumura, T., Ohya, K., Ichouda, N., Sugimoto C.: "Expression of human interferon alfa and tumor necrosis factor alfa in plants"Molecurar Farming (Toutant, J.P. & Balazs, E. ed; (INRA Editions, France). 177-185 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohya, Matsumura, T., K., Ichouda, N., Ohashi, K., Onumna, M., Sugimoto C.: "Protective effect of orally administrated human interferon alfa against Listeria monocytogenes infection and practical advantage of HulFN-alfa derived from transgenic potato plants"Plant Biotechnology 2002 and Beyond (I.K. Vasil, ed.)(Kluwer Academic Publishers)(ISBN 1-4020-1126-1). 389-392

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sugimoto C., Ueda, S., Masuta, C., Ohya, K., Matsumura, T., Uyeda, I.: "Development of a new plant virus vector system and expression of human interferon alfa in broad beans")olecurar Farming (Toutant, J.P. & Balazs, E. ed; INRA Editions, France). 173-177 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsumura, T., Ohya, K., Ichouda, N., Sugimoto C.: "Expression of human interferon alfa and tumor necrosis factor alfa in plants"Molecurar Farming (Toutant, J.P. & Balazs, E. ed;INRA Editions, France). 177-185 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ohya K. Matsumura T. Ohashi K. Onuma M. Sugimoto C.: "Expression of two subtypes of human IFN-alpha in transgenic potato plants"Journal of Interferon & Cytokine Research. 21・8. 595-602 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hataya T. Arimoto R. Suda N. Uyeda I: "Molecular characterization of Hop mosaic virus : its serological and molecular relationships to Hop latent virus"Archives of Virology. 146・10. 1935-1948 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hataya T. Uchino K. Arimoto R. Suda N. Sano T. Shikata E. Uyeda I.: "Molecular characterization of Hop latent virus and phylogenetic relationships among viruses closely related to carlaviruses"Archives of Virology. 145・12. 2503-2524 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Masuta C. Yamana T. Tacahashi Y. Uyeda I. Sato M. Ueda S. Matsumura T: "Development of clover yellow vein virus as an efficient, stable gene-expression system for legume species"Plant Journal. 23・4. 539-546 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mulenga A. Sugimoto C. Ingram G. Ohashi K. Misao O.: "Characterization of two cDNAs encoding serine proteinases from the hard tick Haemaphysalis longicornis"Insect Biochemistry & Molecular Biology. 31・8. 817-825 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Inoue M. Van Nguyen D. Meas S. Ohashi K. Sen S. Sugimoto C. Onuma M.: "Survey of Theileria parasite infection in cattle in Cambodia and Vietnam using piroplasm surface protein gene-specific polymerase chain reaction"Journal of Veterinary Medical Science.. 63・10. 1155-1157 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tsuda A. Mulenga A. Sugimoto C. Nakajima M. Ohashi K. Onuma M.: "cDNA cloning, characterization and vaccine effect analysis of Haemaphysalis longicornis tick saliva proteins"Vaccine. 19. 4287-4296 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小沼 操、ムレンガーアルバート、中島美枝、杉本千尋: "ダニの吸血を阻害する抗ダニワクチン"動薬研究. 12. 11-23 (1999)

    • Related Report
      2001 Annual Research Report
  • [Publications] Rhakha, N.K., Sugimoto, C., & Onuma, M: "Recent development in diagnosis and prophylaxis of Theleria annulata and T. sergenti infections"Curr. Trend Immunol. 2. 135-144 (1999)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mulenga, A, Sugimoto, C. & Onuma, M: "Issues in tick vaccine development:identification and characterization of potencial candidate vaccine antigens"Microbe & Infection. 2. 1353-1361 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mulenga, A, Sugino, M., Nakajima, M, Sugimoto, C. & Onuma, M: "Tick-encoded proteinase inhibitors (Serpins); potencial target antigens for tick vaccine development"J. Vet. Med. Sci.. 63. 1063-1069 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] C. Sugimoto and K. Fujisaki (2001) USA: "Non-transforming Theileria parasites in ruminants In:Theileria"(D. Dobbelaere ed., Kluwer Academic Publishers. 10 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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