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Efficacy study of Plasmodium vivax transmission-blocking vaccine on field isolates

Research Project

Project/Area Number 12557026
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 寄生虫学(含医用動物学)
Research InstitutionEhime University

Principal Investigator

TSUBOI Takafumi  Ehime University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (00188616)

Co-Investigator(Kenkyū-buntansha) TORII Motomi  Ehime University, Faculty of Medicine, Professor, 医学部, 教授 (20164072)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2001: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2000: ¥8,500,000 (Direct Cost: ¥8,500,000)
KeywordsPlasmodium vivax / transmission-blocking vaccine / ookinete / 国際研究者交流 / タイ
Research Abstract

Most leading malaria transmission-blocking vaccine candidate antigens are surface proteins expressed on zygotes and ookinetes of the malaria parasites. Two prime vaccine candidates are Pfs25 and Pfs28, ookinete surface proteins of Plasmodium falciparum. P. vivax homologues, Pvs25 and Pvs28, have been cloned, and expressed in yeast as vaccine antigens. These elicit potent transmission-blocking activity to P. vivax Sall strain. To test the efficacy of this vaccine candidate in the natural parasite populations, antisera against yeast expressed recombinant proteins, yPvs25 and yPvs28, were produced in mice and rabbits. The efficacies of the antisera were tested with human isolates at malaria clinics in northwestern Thailand. For most human isolates, sera from mice immunized using alum as adjuvant showed complete inhibition of oocyst development. Sera from rabbits immunized with yPvs25 or yPvs28 + alum was less inhibitory than the mouse sera. Sera from rabbits immunized with yPvs25 or yPvs28 + Freund's adjuvant was more inhibitory, but still less than the mouse sera. The inhibitory activity correlated with the antibody titer measured by ELISA on recombinant protein or IFA on cultured ookinetes. Genotypes of Pvs25 and Pvs28 from the isolates tested for transmission blocking were determined. Although Pvs25 gene was highly conserved, three amino acid substitutions were found. Pvs28, was more polymorphic than Pvs25. Ten amino acid substitutions and the different numbers of repeats at the end of the fourth EGF-like domain were found. There was no correlation between genotype and transmission blocking.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Hisaeda H. et al.: "Antibodies to malaria vaccine candidates Pvs25 and Pvs28 completely block the ability of Plasmodium vivax to infect mosquitoes"Infect. Immun.. 68. 6618-6623 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shirano M. et al.: "Conserved regions of the Plasmodium yoelii rhoptry protein RhopH3 revealed by comparis on with the P. falciparum homologue"Mol. Biochem. Parasitol.. 112. 298-299 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tachibana M. et al.: "Presence of three distinct ookinete surface protein genes, Pos25, Pos28-1, and Pos28-2, in Plasmodium ovale"Mol. Biochem. Parasitol.. 113. 341-344 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yuda M.et al.: "von Willebrand Factor A Domain-related Protein, a novel microneme protein of the malaria ookinete highly conserved throughout Plasmodium parasites"Mol. Biochem. Parasitol.. 116. 65-72 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suwanabun N. et al.: "Development of a method for the in vitro production of Plasmodium vivax ookinetes"J. Parasitol.. 87. 928-930 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneko O. et al.: "The high molecular mass rhoptry protein, RhopH1, is encoded by members of the clag multigine family in Plasnodium falciparum and P. yoelii"Mol. Biochem. Parasitol.. 118. 237-245 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hisaeda H. et al.: "Antibodies to malaria vaccine candidates Pvs25 and Pvs28 completely block the ability of Plasmodium vivax to infect mosquitoes"Infect. Immun.. 68. 6618-6623 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shirano M. et al.: "Conserved regions of the Plasmodium yoelii rhoptry protein RhopH3 revealed by comparis in with the P.falciparum homologue"Mol. Biochem. Parasitol.. 112. 298-299 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tachibana M. et al.: "Presence of three distinct ookinete surface protein genes, Pos25, Pos28-1, and Pos28-2, in Plasmodium ovale"Mol. Biochem. Parasitol.. 113. 341-344 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yuda M. et al.: "von Willebrand Factor A Domain-related Protein, a novel microneme protein of the malaria ookinete highly conserved throughout Plasmodium parasites"Mol. Biochem. Parasitol.. 116. 65-72 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suwanabun N. et al.: "Development of a method for the in vitro productin of Plasmodium vivax ookinetes"J. Parasitol.. 87. 928-930 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneko O. et al.: "The high molecular mass rhoptry protein, PhopH1, is encoded by members of the clag multigine family in Plasnodium falciparum and P. yoelii"Mol. Biochem. Parasitol.. 118. 237-245 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hisaida H. et al.: "Antibodies to malaria vaccine candidates Pvs25 and Pvs28 completely block the ability of Plasmodium vivax to infect mosquitoes"Infect. Immun.. 68. 6618-6623 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shirano M. et al.: "Conserved regions of the Plasmodium yoelii rhoptry protein RhopH3 revealed by comparis on with the P. falciparum homologue"Mol. Biochem. Parasitol.. 112. 298-299 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tachibana M. et al.: "Presence of three distinct ookinete surface protein genes, Pos25, Pos28-1, and Pos28-2, in Plasmodium ovale"Mol. Biochem. Parasitol.. 113. 341-344 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yuda M.et al.: "von Willebrand Factor A Domain-related Protein, a novel microneme protein of the malaria ookinete highly conserved throughout Plasmodium parasites"Mol. Biochem. Parasitol.. 116. 65-72 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Suwanabun N. et al.: "Development of a method for the in vitro production of Plasmodium vivax ookinetes"J. Parasitol.. 87. 928-930 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kaneko O. et al.: "The high molecular mass rhoptry protein, RhopH1, is encoded by members of the clag multigine family in Plasnodium falciparum and P. yoelii"Mol. Biochem. Parasitol.. 118. 237-245 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tachibana M. et al.: "Presence of three distinct ookinete surface protein genes, Pos25, Pos28-1, and Pos28-2, in Plasmodium ovale."Mol.Biochem.Parasitol.. (発表予定). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hisaeda H. et al.: "Antibodies to malaria vaccine candidates Pvs25 and Pvs28 completely block the ability of Plasmodium vivax to infect mosquitoes."Infect.Immun.. 68. 6618-6623 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Langer R.C. et al.: "Micronemal transport of Plasmodium ookinete chitinases to the electron-dense area of the apical complex for extracellular secretion."Infect.Immun.. 68. 6461-6465 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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