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Development of bioremediation of remaining dioxins using Sea elegans

Research Project

Project/Area Number 12557033
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Hygiene
Research InstitutionHokkaido University

Principal Investigator

KURASAKI Masaaki  Hokkaido Univ., Grad. School of Environ. Sci., Instructor, 大学院・地球環境科学研究科, 助手 (80161727)

Co-Investigator(Kenkyū-buntansha) HOSOKAWA Toshiyuki  Hokkaido Univ., Center of Higher Education, Associate Prof., 高等教育機能開発総合センター, 助教授 (00157025)
ITO Keizo  Frontier Science Inc., Chief Director, 技術研究所, 所長 (40213037)
SAITO Takeshi  Hokkaido Univ., Grad. School of Med, Associate Prof., 大学院・医学研究科, 助教授 (40153811)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2001: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2000: ¥8,000,000 (Direct Cost: ¥8,000,000)
Keywordsdioxins / bioremediation / reporter assay / Bacillus subtilis / Phanerochaete chrysosporium / DNA microarray / endocrine disrupers / genetic engineering / 測定系の構築 / 分解系の構築 / CYP1A1転写制御領域 / 培養細胞
Research Abstract

Recently, environmental pollutant by dioxins, one of the endocrine disrupters has been considered as dysfunctional factor of mutagenesis, and to affect on development and differentiation of fetals. In this study, to confirm the system of measurement and bioremediation of dioxins in environmental by using of genetic engineering.
As results, we have successful developed convenience measurement system of dioxins in environment. The detection range of dioxins using the developed reporter assay system showed 10 fmol to 100 pmol. To consruct the system of bioremediation of dioxins, the genes and cDNAs of enzymes that were expected to degrade the dioxins, has been cloned from Bacillus subtilis and Phanerochaete chrysosporium. The enzymes were expressed in Esherichia coli, and the dioxin degraded activity of the expressed enzymes was estimated by using the developed reporter assay above mentioned. From the results, some enzymes showed the activity of degradation of dioxins. These results obtained in this study are expected to contribute the establishment of bioremediation of remaining dioxins in environment.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Osamu Yamanoshita: "Diverse effect of tributyltin on apoptosis in PC12 cells"Biochemical and Biophysical Research Communications. 272. 557-562 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masaaki Kurasaki: "Metals in water in the Central Kalimantan, Indonesia"Bulletin of Environmental Contamination and Toxicology. 65. 591-597 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeshi Saito: "Apoptosis and Endocrine Disrupters"Biomedical Research. 21. 353-359 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Osamu Yamanoshita: "2,4,5-trichlorophenoxy acetic acid inhibits apoptosis in PC12 cells"Life Sciences. 69. 403-408 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yu-Ping Wang: "Changes in middle latency auditory-evoked potentials of the rat exposed to styrene"Journal of Health Science. 47. 505-511 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masaaki Kurasaki: "A developed method of terminal deoxynucleotidyl transferase system for quantification of DNA damage caused by apoptosis"Analytical Sciences. 17Supple.. i1547-i1550 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masaki Ohta: "The effect of trichloethylene on spatio-temporal pattern of LTP in mouse hippocampal slices"International Journal of Neuroscience. 111. 257-271 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 斎藤 健: "微量化学物質による中枢神経障害の評価"日本内分泌撹乱物質学会ニュースレター. 3. 4 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 蔵崎正明: "培養細胞を用いた環境ホルモンの生体影響評価法の確立"ガイア. 5. 3 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 斎藤 健: "遺伝子工学的手法による残留ダイオキシン類の低減化法の開発に関する研究"第1回廃棄物処理科学シンポジウム-循環型社会の創造に向けて-プロシーディング集. 89-94 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shigeru Saito: "Localization of the induced metallothionein and DNA damage in rat kidney after gold injection"Journal of Histochemistry and Cytochemistry. 50. 1263-1271 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takuya Numata: "Bcl-2 linked apoptosis due to increase in NO synthase in brain of SAMP10"Biochemistry and Biophysics Research Communications. 297. 517-522 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Osamu Yamanoshita, Masaaki Kurasaki, Takeshi Saito, Kyoko Takahashi, Hiroko Sasaki, Toshiyuki Hosokawa, Masashi Okabe, Junko Mochida, Toshio Iwakuma: "Diverse effect of tributyltin on apoptosis in PC12 cells"Biochemical and Biophysical Research Communications. 272. 557-562 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masaaki Kurasaki, Dede Irvin Hartoto, Takeshi Saito, Mika Suzuki-Kurasaki, Toshio Iwakuma: "Metals in water in the Central Kalimantan, Indonesia"Bulletin of Environmental Contamination and Toxicology. 65. 591-597 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeshi Saito, Masaaki Kurasaki: "Apoptosis and Endcrine Disrupters"Biomedical Research. 21. 353-359 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Osamu Ymanoshita, Takeshi Saito, Kyoko Takahashi, Hiroko Sasaki, Toshiyuki Hosokawa, Masashi Okabe, Keizo Ito, Masaaki Kurasaki: "2, 4, 5-trichlorophenoxy acetic acid inhibits apoptosis in PC12 cells"Life Sciences. 69. 403-408 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yu-Ping Wang, Takeshi Saito, Toshiyuki Hosokawa, Masaaki Kurasaki, Kazuo Saito: "Changes in middle latency auditory-evoked potentials of the rat exposed to styrene"Journal of Health Sciences. 47. 505-511 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masaaki Kurasaki, Miho Aoki, Tomoe Miura, Sayaka Seki, Shigeru Toriumi, Toshiyuki Hosokawa, Masashi Okabe, Takuya Numata, Shigeru Saito, Takeshi Saito: "A developed method of terminal deoxynucleotidyl transferase system for quantification of DNA damage caused by apoptosis"Analytical Sciences 17. Supple.. i1547-i1550 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masaki Ohta, Takeshi Saito, Kazuo Saito, Masaaki Kurasaki, Toshiyuki Hosokawa: "The effect of trichloethylene on spatio-temporal pattern of LTP in mouse hippocampal slices"International Journal of Neuroscience. 111. 257-271 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeshi Saito, Masaaki Kurasaki, Toshiyuki Hosokawa, Hiroyoshi Fujita: "Evaluation of dysfunctional effects in Central nervous system by environmental contaminants(in Japanese)"News letters of Meeting of Endocrine disrupters.. 3,4. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masaaki Kurasaki: "Evaluation of bioeffects of endocrine disrupters using of cultured cells(in Japanese)"Gaiya. 5,3. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeshi Saito, Masaaki Kurasaki, Hiroyoshi Fujita, Keizo Ito: "Development of biodegradation of dioxins by using of genetic engineering(in Japanese)"Proceedings of First Symposium of Environmental contaminants. 89-94. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shigeru Saito, Masaaki Kurasaki, Toshiyuki Hosokawa, Masashi Okabe, Takeshi Saito, Yukiko Fujii, Kazuo Nagashima, Katsumi Yoshida: "Localization of the induced metallothionein and DNA damage in rat kidney after gold injection"Journal of Histochemistry and Cytochemistry. 50. 1263-1271 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takuya Numata, Takeshi Saito, Kazuyo Maekawa, Yasumitsu Takahashi, Hiroshi Saitoh, Toshiyuki Hosokawa, Hiroyoshi Fujita, Masaaki Kurasaki: "Bcl-2 linked apoptosis due to increase in NO synthase in brain of SAMP10"Biochemistry and Biophysics Research Communications. 297. 517-522 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Numata et al.: "Bcl-2-liuked apoptosis due to increase in NO synthase in brain of SAMP 10"Biochemical and Biophysical Research Communications. 297(3). 517-522 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Sato et al.: "Protective effect of taurine against renal interstitial fibrosis of rats induced by cisplatin"Naunyn-Schmiedeberg's Archives of Pharmacology. 365. 277-283 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Saito et al.: "Localization of the induced metallothionein and DNA damage in rat kidney after gold injection"Journal of Histochemistry and Cytochemistry. 50. 1263-1271 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Kurasaki et al.: "A developed method of terminal deoxynucleotidyl transferase system for quantification of DNA damage caused by apoptosis"Analytical Sciences. 17supplement. i1547-i1550 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 蔵崎正明: "培養細胞を用いた環境ホルモンの生体影響評価法の構築"ガイア. 5. 3 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamanoshita, O. et al.: "2,4,5-Trichlorophenoxyacetic acid inhibits apoptosis in PC12 Cells"Life Sciences. 69. 403-408 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kurasaki, M. et al.: "A developed method of terminal deoxynucleotidyl transferase system for quantification of DNA damage caused by apoptosis"Analytical Sciences. (In press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Saito., T, Kurasaki, M.: "Apoptosis and endocrine disrupters"Biomedical Research. 21. 353-359 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hosokawa, T. et al.: "Effect of tributyltin on apoptosis in PC12 cells"Society for neuroscience Abstracts. 27. 109 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 齋藤 健, 蔵崎正明 他: "微量化学物質による中枢神経障害の評価"日本内分泌撹乱物質学会ニュース. 3 (4). 4 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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