| Project/Area Number |
12557044
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
内科学一般
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| Research Institution | Tohoku University |
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Principal Investigator |
SASAKI Takeshi Tohoku Univ., Medicine, Professor, 大学院・医学系研究科, 教授 (50110656)
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| Co-Investigator(Kenkyū-buntansha) |
KAMEOKA Junichi Tohoku Univ. Hospital, Research Associate, 医学部・付属病院, 助手 (30261621)
HARIGAE Hideo Tohoku Univ. Hospital, Research Associate, 医学部・付属病院, 助手 (50302146)
KITAGAWA Yoshichika Akita Univ., Ins Biotech., Professor, 生物工学研究所, 教授 (10006752)
MUNAKATA Yasuhiko Tohoku Univ. Hospital, Research Associate, 医学部・付属病院, 助手 (20271950)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 2001: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 2000: ¥7,300,000 (Direct Cost: ¥7,300,000)
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| Keywords | Gene therapy / antinucleic acid antibody / retrovirus / adult T cell leukemia virus / レトロウィルス / 成人型T細胞 / 人工単鎖抗体 / VH,VL / ヒトT細胞白血病ウイルス / V遺伝子 |
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| Research Abstract |
1.Expression of antibody against DNARNA hybrid in the cells.
We first obtained hybridoma producing monoclonal anti-DMA RNA hybrid antibody and prepared the fragments of VH and VL gene encoding the antibody. Both fragments were bound for the expression of ScFv antibodies and introduced into vectors for the expression in the cells.
2. Efficacy of gene therapy using anti-ScFv expression system for prevention of tabacco mosaic virus (TMV) infection.
Tabacco which was introduced with anti-DNARNA hybrid ScFv genes, expressed ScFv mRNA and showed marked resistance to TMV infection.
3. Blocking experiment of human adult T cell leukemia virus (HTLV-1) infection by gene therapy using anti-ScFv expression system.
We are establishing for evaluation of efficacy of ScFv strategy in order to block HTLV-1 infection in T cells.
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