• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of Pencil-type CCD Microscope for evaluating of renal glomerular microcirculation with normal and hypertensive Rat

Research Project

Project/Area Number 12557065
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Circulatory organs internal medicine
Research InstitutionKawasaki College of Allied Health Professions

Principal Investigator

TACHIBANA Hiroyuki  Kawasaki College of Allied Health Professions, Department of Medical Engineering, Research Asscociates, 臨床工学科, 助手 (00241216)

Co-Investigator(Kenkyū-buntansha) HIRAMATSU Osamu  Kawasaki College of Allied Health Professions, Department of Medical Engineering, Lecture, 臨床工学科, 講師 (50208849)
MATSUMOTO Takeshi  Kawasaki College of Allied Health Professions, Department of Medical Engineering, Associate Professor, 臨床工学科, 助教授 (30249560)
GOTO Masami  Kawasaki College of Allied Health Professions, Department of Medical Engineering, Professor, 臨床工学科, 教授 (50148699)
KATAOKA Noriyuki  Kawasaki College of Allied Health Professions, Department of Medical Engineering, Lecture, 臨床工学科, 講師 (20250681)
MOCHIZUKI Seiichi  Kawasaki College of Allied Health Professions, Department of Medical Engineering, Associate Professor, 臨床工学科, 助教授 (60259596)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥9,500,000 (Direct Cost: ¥9,500,000)
Fiscal Year 2001: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2000: ¥7,100,000 (Direct Cost: ¥7,100,000)
KeywordsCCD Microscope / glomerular microcirculation / Velocity mapping / 速度マッピング / 糸球体毛細管 / 高速度CCD生体顕微鏡 / 腎微小循環 / 血流動態 / 光マーカー
Research Abstract

To evaluate hemodynamics of renal glomerular microcirculation, we developed a pencil-type CCD microscope for evaluating of renal glomerular microcirculation with normal and hypertensive Rat. The system consists of a pencil lens with light guides, a camera body, a control unit, light source, a monitor and a videocassette recorder. The tapered pencil lens (tip diameter : 1mm) contains relay lens surrounded by 8 annular optical-fiber light guides. The spatial resolution of this system is 0.86μm, and the temporal resolution is 16.7ms (60 frames/sec.). The focal depth is 20μm. The pencil lens probe was introduced into the superficial layer of kidney through the incision after the surgical procedure. Image of glomeruli and the adjacent afferent and efferent arterioles were continuously recorded on a videocassette tape. The time-sequential images of therenal zicrocirculation were digitized by a computer system. To measure erythrocyte velocities, a line segment was set along the glomerular capillaries in time-sequential digitized images and then a spatiotemporal image was constructed along that segment. The angle of striped pattern in the spatiotemporal image which reflects the erythrocyte velocity, was estimated to compute erythrocyte velocity vector mapping. We have succeeded in quantitatively evaluating the glomerular microcirculation by direct in-vivo observation with our intravital videomicroscope and image analyzing method. The spatiotemporal method is useful to evaluate the hemodynamic characteristic of the glomerular microcirculation in a rat, normal and abnormal.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 小笠原康夫, 立花博之, 松本健志, 梶谷文彦: "micro-CTを用いた腎糸球体容量分布の解析"第16回生体・生理工学シンポジウム講文集. 0. 195-196 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 小笠原康夫, 立花博之, 松本健志, 梶谷文彦: "腎微小循環系血管容量分布の解析-micro-CTによる糸球体の立体的可視化-"第49回レオロジー討論会講演要旨集. 0. 5-6 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Mochizuki, Y.Chiba, Y.Ogasawara, N.Kataoka, M.Goto, H.Tachibana, F.Kajiya: "Direct in situ evaluation of nitroglycerin-derived nitric oxide production in the canine and rat vascular walls at high temporal and spatial resolutions"Cardiovascular Engineering : An International Journal. 1・2. 85-91 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S, Mochizuki, Y, Chiba, Y, Ogasawara, N, Kataoka, M, Goto, H, Tachibana, F, Kajiya: "Direct in situ evaluation of nitroglycerin-derived nitric oxide production in the canine and rat vascular walls at high temporal and spatial resolutions"Cardiovascular Engineering : An International Journal. 1-2. 85-91 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] D, Merkus, I, Vergrossen, 0, Hiramatsu, H, Tachibana, H, Nakamoto, E, Toyota, M, Goto, Y, Ogasawara, J, A, E, Spaan, F, Kajiya: "Stenosis differentially affects subendocardial and subepicardial arterioles in vivo."American Jouenal of Physiology. 280-4. H1676-H1682 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T, Matsumoto, H, Tachibana, Y, Ogasawara, F, Kajiya: "New double-tracer digital radiography for analysis of spatial and temporal myocardial flow heterogeneity."American Journal of Physilogy. 280-2. H465-H474 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T, Kajita, Y, Ogasawara, T, Matsumoto, H, Tachibana, H, Nakamoto, N, Kataoka, F. Shigeto, C, Tokuda, F, Kajiya: "Spatial correlation of mitochondorial function and flow evaluated with the resolution of microcirculatory flow control unit size in beating heart."Japanese Circulation Journal. 65. 473 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 小笠原康夫, 立花博之, 松本健志, 梶谷文彦: "micro-CTを用いた腎糸球体容量分布の解析"第16回生体・生理工学シンポジウム論文集. 0. 195-196 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小笠原康夫, 立花博之, 松本健志, 梶谷文彦: "腎微小循環系血管容量分布の解析-micro-CTによる糸球体の立体的可視化-"第49回レオロジー討論会講演要旨集. 0. 5-6 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Mochizuki, Y.Chiba, Y.Ogasawara, N.Kataoka, M.Goto, H.Tachibana, F.Kajiya: "Direct in situ evaluation of nitroglycerin-derived nitric oxide production in the canine and rat vascular walls at high temporal and spatial resolutions"Cardiovascular Engineering : An International Journal. 1・2. 85-91 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小笠原康夫,梶田達也,仲本博,松本健志,立花博之,望月精一,梶谷文彦: "低酸素時における冠微小循環とミトコンドリア代謝機能の空間的関連性の解析"Japanese Circulation Journal. 64. 383 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Ogasawara,T.Kajita,T.Matsumoto,H.Tachibana,H.Nakamoto,S.Mochizuki,C.Tokuda,F.Kajiya: "Imaging of micromyocardial metabolic activity and microvascular blood flow distribution"Digest of Papers of the 2000 World Congress on Medical Physics and Biomedical Engineering. WEBa327-07 (2000)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi