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Development of a method to measure viscoelasticity of cardiomyocytes using scanning probe microscope

Research Project

Project/Area Number 12557066
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Circulatory organs internal medicine
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

SUGIMACHI Masaru  National Cardiovascular Center Research Institute, Cardiovascular Dynamics, Senior Staff, 循環動態機能部, 室長 (40250261)

Co-Investigator(Kenkyū-buntansha) SHISHIDO Toshiaki  National Cardiovascular Center Research Institute, Cardiovascular Dynamics, Staff, 循環動態機能部, 室員 (60300977)
SUNAGAWA Kenji  National Cardiovascular Center Research Institute, Cardiovascular Dynamics, Director, 循環動態機能部, 部長 (50163043)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 2001: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥3,700,000 (Direct Cost: ¥3,700,000)
Keywordsscanning probe microscope / minute vibration / time-varying elastance / viscosity / cardiomyocyte / laminin / collagenase / biopsy specimen
Research Abstract

We developed a method to measure viscoelasticity of cardiomyocytes from animals. Rats were anesthetized with pentobarbital, and the hearts were retrogradely perfused with collagenase to isolate cells. The cells were incubated for 4 hour (37℃, 5 %CC_2) in a laminin-coated dish, during which the cells firmly attached to the dish. We made a circuit and modified a scanning probe microscope so that we were able to control the vertical position of the probe with a piezoelectric device according to external command. We also developed a software to impose command and to measure the position of the piezoelectric device and the bending of the cantilever. When we imposed a sinusoidal vibration, the bending of the cantilever was in phase, indicating the viscosity of the superfusate is negligible.
We measured the changes in elasticity of cells during cardiac cycle by subjecting the cell to electrical stimulation, but the results indicated no significant changes. As it is known osmotic stress do change elasticity, we conjectured that the probe detects the local elasticity, and the changes in probe size seem necessary to detect the myocardial cellular elasticity changes. The lack of preload seems to contribute the small changes in elasticity.
We were not able to collect sufficient healthy cells either by superfusion with collagenase with or without ultrasonic agitation, or super fusion with protease.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Masaru Sugimachi: "Effects of partial left ventriculectomy on left ventricular pump function studied by theoretical analyses"J Card Surg. 16. 24-39 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masaru Sugimachi: "A new model-based method of reconstructing central aortic pressure from peripheral arterial pressure"Jpn J Physiol. 51. 217-222 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masaru Sugimachi: "Lower compliance rather than high reflection of arterial system decreases stroke volume in arteriosclerosis : A simulation"Jpn J Physiol 2001;51:43-51. 51. 43-51 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasunori Nakayama: "Heart rate-independent vagal effect on end-systolic elastance of the canine left ventricle under various levels of sympathetic tone"Circulation. 104. 2277-2279 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Toshiaki Shishido: "Single-Beat Estimation of End-Systolic Elastance Using Bilinearly Approximated Time-Varying Elastance Curve"Circulation. 102. 1769-1776 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kazuko Hayashi: "Single-beat estimation of Ees/Ea as an index of ventricular mechano-energrtic performance"Anesthesiology. 92. 1769-1776 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sugimachi M et al: "Effects of partial left ventriculectomy on lfet ventricular pump function studied by theoretical analyses"J Card Surg. 16. 24-29 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sugimachi M et al: "A new model-based method of reconstructing central aortic pressure from peripheral arterial pressure"Jpn J Physiol. 51. 217-222 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sugimachi M et al: "Lower compliance rather than high reflection of arterial system decreases stroke volkume in arteriosclerosis : A simulation"Jpn J Physiol. 51. 43-51 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakayama Y et al: "Heart rate-independent vagal effect on end-systolic elastance of the canine left ventricle under various levels of sympathetic tone"Circulation. 104. 2277-2279 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shishido T et al: "Single-Beat Estimation of End-systolic Elastance Using Bilinearly Approximated Time-Varying Elastance Curve"Circulation. 102. 1983-1989 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hayashi K et al: "Single-beat estimation of Ees/Ea as an index of ventricular mechano-energrtic performance"Anesthesiologyh. 92. 1769-1776 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masaru Sugimachi: "Effects of partial left ventriculectomy on left ventricular pump function studied by theoretical analyses"J Card Surg. 16. 24-39 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Masaru Sugimachi: "A new model-based method of reconstructing central aortic pressure from peripheral arterial pressure"Jpn J Physiol. 51. 217-222 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Masaru Sugimachi: "Lower compliance rather than high reflection of arterial system decreases stroke volume in arteriosclerosis : A simulation"Jpn J Physiol 2001;51:43-51. 51. 43-51 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yasunori Nakayama: "Heart rate-independent vagal effect on end-systolic elastance of the canine left ventricle under various levels of sympathetic tone"Circulation. 104. 2277-2279 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yasunori Nakayama: "Laterality in direct and indirect inotropic effects of sympathetic stimulation in isolated canine heart"Jpn J Physiol. 51. 365-370 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akira T Kawaguchi: "Perioperative hemodynamics in patients undergoing partial left ventriculectomy"J Card Surg. 16. 48-55 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sugimachi M: "Effects of partial left ventriculectomy on left ventricular pump function studied by theoretical analyses."J Card Surg. (In press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Sugimachi M: "A new model-based method of reconstruction central aortic pressure from peripheral arterial pressure."Jpn J Physiol. (In press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Sugimachi M: "Lower compliance rather than high reflection of arterial system decreases stroke volume in arteriosclerosis : A simulation."Jpn J Physiol. (In press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Shishido T: "Single-Beat Estimation of End-Systolic Elastance Using Bilinearly Approximated Time-Varying Elastance Curve."Circulation. 102. 1983-1989 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Miyashita H: "A novel servo control system that imposes desired aortic input impedance on the in situ rat heart."Am J Physiol (Heart Circ Physiol). 278. H998-H1007 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hayashi K: "Single-beat estimation of Ees/Ea as an index of ventricular mechano-energrtic performance."Anesthesiology. 92. 1769-1776 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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