HOMEOTIC TRANSCRIPTION FACTORS THAT BIND TO FOLLICLE -STIMULATING HORMONE SUBUNITE GENE

• Project/Area Number: 12557087
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 展開研究
• Research Field: Endocrinology
• Research Institution: SCHOOL OF AGRICULTURE
• Principal Investigator: KATO Yukio MEIJI UNIVERSITY, SCHOOL OF AGRICULTURE, PROFESSOR, 農学部, 教授 (30114177)
• Project Period (FY): 2000 – 2003
• Project Status: Completed (Fiscal Year 2003)
• Budget Amount: ¥9,600,000 (Direct Cost: ¥9,600,000); Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000); Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000); Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000); Fiscal Year 2000: ¥4,500,000 (Direct Cost: ¥4,500,000)
• Keywords: PITUITARY / RECOMBINANT DNA / GONADOTROPIN / CLONING / TRANSCRIPTION FACTOR / GENE / HOMEOBOX / YEST ONE HYBRID / 相互作用 / 遺伝子 / 生殖 / ホルモン

Research Abstract

(1)FOUR TRANSCRIPTION FACTORS THAT BIND TO THE UPSTREAM REGION OF PORCINE FOLLICLE STIMULATING HORMONE SUBUNIT GENE HAVE BEEN CLONED USING YEAST ONE-HYBRID SYSTEM.
(2)THE NUCEOTIDE SEQUENCES OF CLONES HAVE HOMEOTIC REGION, WHICH RECOGNIZES SPECIFIC DNA SEQUENCES.
(3)PRODUCTION OF RECOMBINANT TRANSCRIPTION FACTOR HAVE BEEN SUCCEEDED AND THE GEL MOBILITY SHIFT ASSAY DEMONSTRATED SPECIFIC BINDING FOR FSHβ GENE
(4)EXPRESSION. VECTORS CONTAINING CLONED TRANSCRIPTION FACTORS WERE TRANSFECTED IN THE CHO CELLS TOGETHER WITH A REPORTER VECTOR FUSED WITH THE 5'-UPSTREAM REGION OF THE FSHI3 GENE AND SOME STIMULATION OF THE GENE EXPRESSIO WAS OBSERVED.

Research Products

• [Publications] Chen, L.: "Cytological characterization of a pituitary folliclo-stellate-like cell line, Tpit/F1, with special reference to adenosine triphosphate-mediated neuronal nitric oxide synthase expression and nitric oxide secretion"Endocrinology. 141. 3603-3610 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nishida, N.: "Critical role of nitric oxide in expression of porcine LH receptor at transcription and post-transcription levels"Experimental and Clinical Endocrinology and Diabetes. 108. 424-429 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nishida, N.: "Transcription facgtor genes, c-fos and c-jun, in developing porcine granulosa cells in vitro"Journa of Reproduction and Development. 46. 167-175 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hattori M.: "FSH suppression of nitric oxide synthesis in porcine oocytes"Journa of Molecular Endocrinology. 24. 65-73 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 加藤幸雄: "成長ホルモン遺伝子とプロラクチン遺伝子の発現スイッチングのリアルタイム測定系の開発"成科学協会研究年報. 23. 167-172 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takesue, K.: "Stimulation of nitric oxide synthesis in porcine granulosa cells by phorbol ester"Journa of Reproduction and Development. 47. 1-6 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nishida, N.: "Expression of endothelial nitric oxide synthase gene in cultured porcine granulosa cells after FSH stimulation"Journal of Molecular Endocrinology. 26. 259-265 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hattori, M-A.: "Expression of endothelial nitric oxide synthetase in the porcine oocyte and its possible function"Molecular and Cellular Biology. 219. 121-126 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hattori, M-A.: "Synthesis of nitric oxide in the porcine ovary and its possible function"Reproductive Biotechnology. 99-103 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kato, Y.: "Presence of an 85 kDa pituitary protein (Pit-G) in porcine GH-producing cells"Journa of Reproduction and Development. 48. 87-95 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hattori MA: "Retinoic acid suppression of endothelial nitric oxide synthase in porcine oocyte"Can J Physiol Pharmacol. 80. 777-782 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hattori, M-A.: "Preferential expression of ADP-ribosylation factor gene in the chick embryonic gonads"Life Science. 70. 2031-2037 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hattori, M-A.: "Prominent expression of transforming growth factor β2 gene in the chicken embryonic gonad as revealed by suppressive subtraction cloning"Gen Comp Endocrinol. 125. 311-316 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Iwasawa A: "Changes in thyrotropin messenger RNA and circulating hormone levels during induced molting of the hen (Gallus domesticus)"J Reprod Dev. 48. 489-496 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Iwasawa A: "Specific anti-peptide antibody to β subunit of chicken thyrotropin : production and characterization"Endocr J. 48. 197-204 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tomizawa K: "Production and Characterization of an Antiserum against Recombinant Porcine Follicle Stimulating Hormone"J Reprod Dev. 48. 131-136 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Aikawa, S., Kato, T., Kato, Y.: "Molecular Cloning of Porcine Neuronatin and Analysis of Its Expression during Pituitary Ontogeny"Exp.Clinic.Endocrinol.Diabetes.. 111. 1-5 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Koizumi, M., Horisuchi, K, Tomita, Y., Kato, Y., Harigaya, T.: "Prolactin gene expression in the mouse nipple."J Reprod.Develop.. 49. 465-472 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Petric, N., Kato, Y., Elsaesser, F.: "Influence of prenatal testosterone treatment on fetal and prepubertal LHs-subunit geneexpression and prepubertal plasma LH concentrations in the female pig"Dom.Anim.Endocrinol.. (印刷中).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Moriyama, R., Tsukamura, H., Kinoshita, M., Okazaki.H., Kato, Y., Maeda, K.-I.: "In vitro Increase In Intracellular Calcium Concentrations Induced by Low or High Extracellular Glucose Levels in Ependymocytes and Serotonergic Neurons of the Rat Lower Brain"Endocrinology. (印刷中).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 本比較内分泌学会 編: "講談社"ブルーバックス「生命をあやつるホルモン」(共著). 20 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Chen L, Maruyama D, Sugiyama M, Sakai T, Mogi C, Kato M, Kurotani R, Shirasawa N, Takaki A, Renner U, Kato Y, Inoue K: "Cytological characterization of a pituitary folliclo-stellate-like cell line, Tpit/F1, with special reference to adenosine triphosphate-mediated neural nitric oxide synthetase expression and nitric oxide secretrion."Endocrinology. 141. 3603-3310 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori M-A, Nishida N, Takesue K, Kato Y, Fujihara N: "Follicle-stimulating hormone suppression of nitric oxide synthesis in the porcine oocytes"J Mol Endocrinol. 24. 65-73 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nishida N, Takesue K, Hattori M-A, Kato Y, Fujihara N: "Critical role of nitric oxide in expression of porcine LH receptor at transcription and post-transcription levels."Exp Clin Endocrinol Diabetis. 108. 424-429 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nishida N, Takesue K, Hattori M-A, Kato Y, Wakabayashi K, Fujihara N: "Modulatory action of nitric oxide on the expression of transcription factor genes, c-fos and c-jun, in developing porcine granulosa cells."J Reprod Dev. 46. 167-175 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shimizu H, Ohtani K, Kato Y, Mori M: "Interleukin-6 increases insulin secretion and preproinsulin mRNA expression via Ca2+-dependent mechanism."J Endocrinol. 166. 121-126 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori M-A, Nishida N, Takesue K, Kato Y, Fujihara N: "Synthesis of nitric oxide in the porcine ovary and its possible function."Reproductive Biotechnology (ed. Miyamoto H N Manabe) (Hokuto Printing Company). 99-103 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori M-A, Takesue K, Kato Y, Fujihara N: "Expression of endbthelial nitric oxide synthetase in the porcine oocyte and its possible function."Mol Cell Biochem. 219. 121-126 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Takesue K, Nishida N, Hattori M-A, Kato Y, Fujihara N: "Stimulation of nitric oxide synthetase in porcine granulosa cells by phorbol ester."J Reprod Dev. 47. 1-6 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori MA, Kato Y, Fujihara N: "Retinoic acid suppression of endothelial nitric oxide synthase in porcine oocyte."Can J Physiol Pharmacol. 80. 777-782 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori M-A, Fujioka T, Kato Y, Fujiwara N: "Preferential expression of ADP-ribosylation factor gene in the chick embryonic gonads."LF. 70. 2031-2037 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori M-A, Furuta H, Hiyama Y, Kato Y, Fujihara N: "Prominent expression of transforming growth factor b2 gene in the chicken embryonic gonad as revealed by suppressive subtraction cloning."Gen Comp Endocrinol. 125. 311-316 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Iwasawa A, Kato Y, Hishikawa T, Doi O, Kamiyoshi M, Nakamura T: "Changes in thyrotropin messenger RNA and circulating hormone levels during induced molting of the hen (Gallus domesticus)."J Reprod Dev. 48. 489-496 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Iwasawa A, Nakayama H, Yamamoto Y, Doi O, Nakamura T, Kato Y: "Specific anti-peptide antibody to b subunit of chicken throtoropin : production and characterization"Endocr J. 48. 197-204 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kato Y, Kato T, Tomizawa K, Ogawa S, Hattori M-A, Inoue K: "Presence of an 85 kDa pituitary protein (Pit-G) in porcine GH-producing cells."J Reprod Dev. 48. 87-95 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tomizawa K, Ogawa S, Kato T, Watanabe M, Sato I, Sakai T, Hattor M-A, Inoue K, Ueda S, Kikuchi M, Mori J, Kato Y: "Production and Characterization of an Antiserum against Recombinant Porcine Follicle Stimulating Hormone"J Reprod Dev. 48. 131-136 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Aikawa S, Kato T, Kato Y: "Molecular Cloning of Porcine Neuronatin and Analysis of Its Expression during Pituitary Ontogeny."Exp.Clinic.Endocrinol.Diabetes. (in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Aikawa, S., Kato, T., Kato, Y.: "Molecular Cloning of Porcine Neuronatin and Analysis of Its Expression during Pituitary Ontogeny"Exp.Clinic.Endocrinol.Diabetes.. 111. 1-5 (2003)
• [Publications] Koizumi, M., Horiguchi, K., Tomita, Y., Kato, Y., Harigaya, T.: "Prolactin gene expression in the mouse nipple."J Reprod.Develop.. 49. 465-472 (2003)
• [Publications] Petric, N., Kato, Y., Elsaesser, F.: "Influence of prenatal testosterone treatment on fetal and prepubertal LHs-subunit geneexpression and prepubertal plasma LH concentrations in the female pig"Dom.Anim.Endocrinol.. (印刷中).
• [Publications] Moriyama, R., Tsukamura, H., Kinoshita, M., Okazaki, H., Kato, Y., Maeda, K.-I.: "In vitro Increase in Intracellular Calcium Concentrations Induced by Low or High Extracellular Glucose Levels in Ependymocytes and Serotonergic Neurons of the Rat Lower Brain"Endocrinology. (印刷中).
• [Publications] Hattori MA: "Retinoic acid suppression of endothelial nitric oxide synthase in porcine oocyte"Can J Physiol Pharmacol. 80. 777-782 (2002)
• [Publications] Hattori, M-A.: "Preferential expression of ADP-ribosylation factor gene in the chick embryonic gonads"Life Science. 70. 2031-2037 (2002)
• [Publications] Hattori, M-A.: "Prominent expression by of transforming growth factor β2 gene in the chicken embryonic gonad as revealed suppressive subtraction clonin"Gen Comp Endocrinol 2002 ; 125 : 311-316. 125. 311-316 (2002)
• [Publications] Iwasawa A: "Changes in thyrotropin messenger RNA and circulating hormone levels during induced molting of the hen (Gallus domesticus)"J Reprod Dev 2002 ; 48 : 489-496. 48. 489-496 (2002)
• [Publications] Iwasawa A: "Specific anti-peptide antibody to βsubunit of chicken thyrotropin : production and characterization"Endocr J. 48. 197-204 (2002)
• [Publications] Tomizawa K: "Production and Characterization of an Antiserum against Recombinant Porcine Follicle Stimulating Hormone"J Reprod Dev 2002 ; 48 : 131-136. 48. 131-136 (2002)
• [Publications] Soh T: "Utilization of central disk of blastoderm and germinal crescent region for production of interspecific germline chimera between chicken and quail"Asian J Andorol 2002 ; 4 :83-6. 4. 83-86 (2002)
• [Publications] Kato Y: "Presence of an 85 kDa pituitary protein (Pit-G) in porcine GH-producing cells"J Reprod Dev 2002 ; 48 : 87-95. 48. 87-95 (2002)
• [Publications] Aikawa S: "Molecular Cloning of Porcine Neuronatin and Analysis of Its Expression during Pituitary Ontogeny"Endocrinol.Diabetes. (印刷中).
• [Publications] 本比較内分泌学会 編: "ブルーバックス「生命をあやつるホルモン」(共著)"講談社. 20 (2003)
• [Publications] Nishida, N.: "Expression of endothelial nitric oxide synthase gene in cultured porcine granulosa cells after FSH stimulation"Journal of Molecular Endocrinology. 26. 259-265 (2001)
• [Publications] Hattori, M-A.: "Expression of endothelial nitric oxide synthetase in the porcine oocyte and its possible function"Molecular and Cellular Biology. 219. 121-126 (2001)
• [Publications] Hattori, M-A.: "Synthesis of nitric oxide in the porcine ovary and its possible function"Reproductive Biotechnology. 1. 99-103 (2001)
• [Publications] Kato, Y.: "Presence of an 85 kDa pituitary protein (Pit-G) in porcine GEl-producing cells"Journal of Reproduction and Development. 48. 87-95 (2002)
• [Publications] Chen,L.: "Cytological characterization of a pituitary folliclo-stellate-like cell line, Tpit/F1, with special reference to adenosine triphosphate-mediated neuronal nitric oxide synthase expression and nitric oxide secretion"Endocrinology. 141. 3603-3610 (2000)
• [Publications] Nishida,N.: "Critical role of nitric oxide in expression of porcine LH receptor at transcription and post-transcription levels"Experimental and Clinical Endocrinology and Diabetes. 108. 424-429 (2000)
• [Publications] Nishida,N.: "Transcription facgtor genes, c-fos and c-jun, in developing porcine granulosa cells in vitro"Journa of Reproduction and Development. 46. 167-175 (2000)
• [Publications] Hattori M.: "FSH suppression of nitric oxide synthesis in porcine oocytes"Journa of Molecular Endocrinology. 24. 65-73 (2000)
• [Publications] 加藤幸雄: "成長ホルモン遺伝子とプロラクチン遺伝子の発現スイッチングのリアルタイム測定系の開発"成科学協会研究年報. 23. 167-172 (2000)
• [Publications] Takesue,K.: "Stimulation of nitric oxide synthesis in porcine granulosa cells by phorbol ester"Journa of Reproduction and Development. 47. 1-6 (2001)