| Project/Area Number |
12557102
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Digestive surgery
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| Research Institution | Tohoku University |
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Principal Investigator |
SASAKI Iwao TOHOKU UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR, 大学院・医学系研究科, 教授 (60125557)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBATA Chikashi TOHOKU UNIV, HOSPITAL, RESEARCH ASSOCIATE, 医学部附属病院, 助手 (30270804)
FUKUSHIMA Kouhei TOHOKU UNIV, SCHOOL OF MEDICINE, RESEARCH ASSOCIATE, 大学院・医学系研究科, 助手 (20271900)
FUNAYAMA Yuji TOHOKU UNIV, HOSPITAL, LECTURER, 医学部附属病院, 講師 (50192315)
内藤 広郎 東北大学, 医学部・付属病院, 講師 (90180223)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2000: ¥10,300,000 (Direct Cost: ¥10,300,000)
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| Keywords | ileo-jejural transposition / total colectomy / diarrhea / gastric emptying / peptide YY / hypersensitivity / peptide YY / ilejejunal transposition / 異排出 / 消化管運動 / Ileo-jojunal transposition |
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| Research Abstract |
We studied the effect of ileo-jejunal transposition (IJT) on gastrointestinal motility and the possible application of IJT to intractable post-colectomy diarrhea. In experiments with dogs, we divided dogs into 3 groups; control (no surgery). IJT (total colectomy + isoperistaltic interposition of the distal ileum into the proximal jejunum), and sham (colectomy + transection & reanastomosis). We measured gastric emptying of solid and liquid separately with 'absolute weight method,' and plasma level of peptide YY (PYY) was measured at the same time. As a result, gastric emptying of the liquid was not different between groups. Gastric emptying of the solid was delayed and plasma PYY was increased in IJT compared to the other 2 groups. Therefore, enhanced plasma PYY was considered to have induced delayed gastric emptying in IJT. We compared fecal water content and character of feces among groups and found that fecal water content in IJT was significantly less than that in sham. In experiments with rats, we also prepared 3 groups (controll, IJT, and sham) as in experiments with dogs. We killed rats 17-20 weeks after the operation and took out the distal ileum. Longitudinal smooth muscle strips were prepared, and isometric tension was measured in tissue chamber. As a result, basal spontaneous contractile activity without any stimulus was decreased in IJT compared to control, and hypersensitivity to cholinergic agonist bethanechol was observed in IJT. We also found that sensitivity to adrenergic agonist norepinephrine and response to electrical field stimulation did not differ between groups. These results revealed the effect of IJT on ileal smooth muscle contractility and suggested the possibility the IJT could improve post-colectomy diarrhea.
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