| Project/Area Number |
12557108
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Digestive surgery
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| Research Institution | Jichi Medical School |
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Principal Investigator |
KONISHI Fumio Department of Surgery, Omiya Medical Center Jichi Medical School, Professor, 医学部, 教授 (20142242)
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| Co-Investigator(Kenkyū-buntansha) |
MASUBUCHI Shigehiko Jichi Medical School Staff Surgeon, 医学部, 助手 (30316533)
KAI Toshihiro Jichi Medical School Lecture, 医学部, 講師 (40260830)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Colorectal Cancer / Genetic changes / Prognosis factor / Microsatellite instability / Lymphocyte infilfration / Microsatellite Instability / 遠隔成績 / 異時性多発大腸癌 / Microsatellite instability / DNAメチル化 |
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| Research Abstract |
Background : TIL is strictly divided into 2 categories. These are lymphocytes in stroma and those in between cancer cells. However comparison of these two has not been well analyzed. Patients and Methods : The materials were derived from patients with colorectal cancer who underwent surgery in Jichi Medical School and Omiya Medical Center. There were 19 HNPCC cases that were compatible with Japanese criteria A and 106 cases of sporadic colorectal caocer cases in either Dukes B or C stage. As microsatellite markers, the global standard 5 markers were selected. Immunohistochemical analysis was performed using the anti CD3, CD4, CD8 and S-100 antibody, and was evaluated according to the degree of infiltration into 3 degrees. Results : As for stroma infiltrating lymphocyte (SIL) in sCRCs, severe infiltration was observed in 20% of MSI-H cases and 12.8% of MSI-L/MSS cases. Whereas, severe infiltration of inter-tumor cell infiltrating lymphocyte (ITCIL)was observe in 41.7% of MSI-H sCRC cases and 4.3% of MSI-L/MSS cases. Thus there was a close correlation between ITCIL severity and increased microsatellite instability (p<0.001). In examination of ITCIL, severe infiltration cases tended to show a better survival rate than a moderate, mild degree cases. Conclusions : The present study suggested that different factors are involved in the infiltration of SIL and ITCIL. Although there were no statistically significant differences, the cumulative survival rates tended to be higher in severe ITCIL cases than in those with moderate and poor ITCIL cases (p<0.09). It was considered that there might be a possibility of having the role of ITCIL, which is closely related to MSI, for a better prognosis after colorectal cancer surgery.
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