| Project/Area Number |
12557117
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
MITANI Akira Kyoto University, College of Medical Technology, Professor, 医療技術短期大学部, 教授 (50200043)
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| Co-Investigator(Kenkyū-buntansha) |
HASEGAWA Mamoru Dynavec Research Incorporation, Managing Director of Research Laboratories, 所長(研究職)
TANAKA Kohichi Tokyo Medical and Dental University, Medical Research Institute. Professor, 難治疾患研究所, 教授 (80171750)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 2002: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | hypothermia / ischemia / neuronal death / gene / hippocambal CA1 / 低温 / 脳虚血 / 保護機構 / 海馬CA1 / ニューロン死防御 |
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| Research Abstract |
It has been known that mild hypothermia prevents neuronal damage against ischemia, however, the mechanism has not been established. In the present study, we assembled a telemeter-based brain temperature control system that allows continuous monitoring and regulating of brain temperature to investigate the neuroprotective effects of hypothermia. A brain temperature transmitter was attached to the gerbil skull and 5-min transient ischemia was induced. The brain temperature was lowered from 1 hour after recirculation and kept at 32℃ for 24 hours. The result showed that this system regulated gerbil brain temperature continuously, and proved that the post-ischemic hypothermia provided effective and long lasting neuroprotection in the hippocampal CA1. Then, we attempted to examine changes of gene expression in gerbil hippocampal CA1 subjected to ischemia and/or hypothermia. Besides numerous ischemia induced gene products such as interferon induced gene 2, GTPase, and Ca<^2+> pump, hypothermia induced specific up regulation or down regulation of several genes including tyrosine phosphatase-receptor type. This result suggests that these genes induced by hypothermia may be used to present a novel strategy for development of preventives/therapeutics of brain stroke.
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