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Detection of prion infectivity of vibrinogen in human blood

Research Project

Project/Area Number 12557118
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Cerebral neurosurgery
Research InstitutionKyushu University

Principal Investigator

MOURI Shirou  Kyushu University, Graduate School of Medical Sciences, Prof., 大学院・医学研究院, 教授 (40117271)

Co-Investigator(Kenkyū-buntansha) KIRA Jyunichi  Kyushu University, Graduate School of Medical Sciences, Prof., 大学院・医学研究院, 教授 (40183305)
KITAMOTO Tetsuyuki  Tohoku Univ., Graduate School of Medicine, Prof., 大学院・医学研究科, 教授 (20192560)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥12,900,000 (Direct Cost: ¥12,900,000)
Fiscal Year 2002: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2000: ¥6,500,000 (Direct Cost: ¥6,500,000)
Keywordsprion / infectivity / bioassay / CJD / blood / CJD / 感染症 / プリオン感染力価
Research Abstract

We developed new transgenic mice (Ki-ChM) introduced human prion gene, that product a human prion protein (PrP). The humanized mice indicated higher sensitivity to human prions. It is possible to diagnose infectivity of the human prion early by detecting abnormal prion protein in follicular dendritic cells. It could be applied to bioassay for prions. To detect infectivity of human prion by bioassay using Ki-ChM mice, we injected into the mice with two cerebral spinal fluid (CSF) and four sera from sporadic Creutzfeldt-Jakob disease (sCJD) patients, and two sera from normal persons as control. We investigated the abnormal PrP deposit in FDCs of spleer or mesenteric lymph nodes on 75 days after intraperitoneal administration. As the results, there were some obscure deposits in FDCs not only CSF and serum from patients but also normal controls. It would not be confirmed the deposits true or false. To confirm them, homogenates of the spleen detecting obscure deposits were inoculated into Ki-ChM mice again. Every inoculated mouse secondary was negative in FDCs.
On the other hand, two cases of CSF inoculated intracerebrally are on going the examination. But there have been no clinical sins observed in 550 days post inoculation. It should need to observe longer period. We are going to detect prion in blood by the other bioassay using more sensitive mice developing.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] 毛利資郎: "プリオン病の感染予防"脳の科学. 22. 751-756 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tateishi J., Kitamoto T., Mohri S., Satoh S., Sato T., Shepherd A., Macnaughton M R.: "Scrapie Removal using Planova Virus Removal Filters"Biologicals. 29. 17-25 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Horiuchi H., Nemoto T., Ishiguro N., Furuoka H., Mohri s., Shinagawa M.: "Biologocal and biochemical characterization of sheep scrapie in Japan"J.Clin.Microbiol.. 40. 3421-3426 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kitamoto T., Mohri S., Ironside J.W., Miyoshi I., Tanaka T., Kitamoto N., Itohara S., Kasai N., Katsuki M., HIguchi J., Muramoto T., Shin R-W.: "Follicular dendritic cell of the knock-in mouse provides a new bioassay for human prions"Biochem.Biophys.Res.Comm.. 294. 280-286 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 毛利資郎: "ヒト・プリオンのバイオアッセイ"臨床検査. 46. 1553-1558 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 毛利資郎, 北本哲之: "ヒト・プリオンバイオアッセイ法の新展開"第121回日本医学会シンポジウム記録集 プリオン病. 100-108 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 毛利資郎: "プリオン病のモデル動物"神経研究の進歩. 47(1). 54-58 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Taguchi Y., Mohri S., Ironside J.W., Muramoto T., Kitamoto T.: "Humanized knock-in mice expressing chimeric PrP showed varied susceptibility to different human prions"Am.J.Pathol.. 163(6). 2585-2593 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] J.Tateishi, T.Kitamoto, S.Mohri, S.Satoh, T.Sato, A.Shepherd, Mr.Macnaughton: "Scrapie Removal using Planova Virus Removal Filters"Biologicals. 29. 17-25 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Horicuhi, T.Nemoto, N.Ishiguro, H.Furuoka, S.Mohri, M.Shinagawa: "Biological and biochemical characterization of sheep scrapie in Japan"J.Clin.Microbiol.. 40. 3421-3426 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kitamoto, S.Mohri, J.W.Ironside, I.Miyoshi, T.tanaka, N.Kitamoto, S.Itohara, N.Kasai, M.Katsuki, J.Higuchi, T.Muramoto, R-W.Shin: "Follicular dendritic cell of the knock-in mouse provides a new bioassay for human prions"Biochem.Biophys.Res.Comm.. 294. 280-286 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Taguchi, S.Mohri, J.W.Ironside, T.Muramoto, T.Kitamoto: "Humanized knock-in mice expressing chimeric PrP showed varied susceptibility to different human prions"Am.J.Pathol.. 163(6). 2585-2593 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Horiuchi H., Nemoto T., Ishiguro N., Furuoka H., Mohri S., Shinagawa M.: "Biological and biochemical characterization of sheep scrapie in Japan"J. Clin. Microbiol.. 40. 3421-3426 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kitamoto T., Mohri S., Ironside J.W., Miyoshi I., Tanaka T., Kitamoto N., Itohara S., Kasai N., Katsuki M., Higuchi J., Muramoto T., Shin R-W.: "Follicular dendritic cell of the knock-in mouse provides a new bioassay for human prions"Biochem. Biophys. Res. Comm.. 294. 280-286 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 毛利資郎: "ヒト・プリオンのバイオアッセイ"臨床検査. 46. 1553-1558 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 毛利資郎: "プリオン病のモデル動物"神経進歩. 42(1). 54-58 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 毛利資郎, 北本哲之: "ヒト・プリオンバイオアッセイ法の新展開"第121回日本医学会シンポジウム記録集 プリオン病. 100-108 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tateishi J: "Scrapie Removal using Planova Virus Removal Filters"Biologicals. 29. 17-25 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 毛利資郎: "プリオン病の感染予防"脳の科学. 22. 751-756 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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