| Project/Area Number |
12557121
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
ARITA Norio Hyogo College of Medicine, Neurosurgery, Professor, 医学部, 教授 (80159508)
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| Co-Investigator(Kenkyū-buntansha) |
IKEMOTO Hideyasu Hyogo College of Medicine, Neurosurgery, Research Associate, 医学部, 助手 (30278824)
NAKANO Atsuhisa Hyogo College of Medicine, Neurosurgery, Research Associate, 医学部, 助手 (70258151)
TAMURA Kazuyoshi Hyogo College of Medicine, Neurosurgery, Assistant Professor, 医学部, 講師 (40340971)
OKANO Hideyuki Keio University School of Medicine, Physiology, Professor, 医学部, 教授 (60160694)
松本 強 兵庫医科大学, 医学部, 講師 (00181777)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2001: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥8,100,000 (Direct Cost: ¥8,100,000)
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| Keywords | neural stem cell / glioblastoma / oligodendroglioma / oligoastrocytoma / ependymoma / medulloblastoma / Musashi1 / GFAP / glioma / ependvmoma / Nestin / Musashi 1 / ependymoma / cell culture / Hu / MSI1 / NG2 / PDGF-αR |
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| Research Abstract |
The origin of glioma is still controversial. Neural stem cells or neural progenitor cells might develop to glioma cells. To investigate the origin of gliomas, we examined the expression of the human Musashi1 homologue (MSI1) and nestin in human glioma tissues and cultured glioma cells. In glioblastomas, MSI1 was expressed in GFAP-negative tumor cells forming foci that were clearly demarcated and surrounded by GEAP-positive cells. Tumor cells arranged in pseudopalisades were also strongly immunoreactive with MSI1 antibodies. These tumor cells are assumed to be actively proliferating, and to have the character of neural stem cells.
Oligoastrocytomas expressed MSI1 and some of them could express Hu (immature neuronal cell marker). Oligoastrocytomas are thought to be derived from more immature and multipotent cells compared with oligodendrogliomas, which could express GFAP, but not MSI1.
Recent study revealed that ependymal cells seem to be the multipotent neural stem cells. Ependymoma ceils
… More
might show the similar characters like their precursor cells. The origin of medulloblastoma cell has been thought as an immature progenitor *** that can give rise to either neuronal or glial cells. We characterized both ependymoma and medulloblastoma cells to investigate their potential ability of differentiation. Ependymoma cells expressed Musashi1, nestin, A2B5 and did not express Tuj1 and Synaptophysin. Long-term cultured ependymoma cells expressed Tuj1 and Synaptophysin. Medulloblastoma cells expressed Musashi1, Nestin, A2B5 and did not express GFAP. Medulloblastoma cells treated with LIF (leukemia inhibitory factor) and BMP-2 (bone morphogenetic protein-2) could also express GFAP, according to the in vitro conditions that neural stem cells could differentiate to astrocytes. Our study suggested that ependymoma cells might be able to differentiate into neural cells and medulloblastoma cells might be able to differentiate into glial cells.
These results may support the hypothesis that glioma cells are derived from multipotent neural stem cells. Less
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