| Project/Area Number |
12557123
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Dental and Medical University |
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Principal Investigator |
NODA Masaki Tokyo Dental and Medical University, Medical Research Institute, Professor, 難治疾患研究所, 教授 (50231725)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJI Kunikazu Tokyo Dental and Medical University, Medical Research Institute, Assistant Professor, 難治疾患研究所, 助手 (20323694)
YAMASHITA Teruhito Tokyo Dental and Medical University, Medical Research Institute, Assistant Professor, 難治疾患研究所, 助手 (90302893)
NOFUJI Akira Tokyo Dental and Medical University, Medical Research Institute, Associate Professor, 難治疾患研究所, 助教授 (00240747)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2001: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2000: ¥9,100,000 (Direct Cost: ¥9,100,000)
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| Keywords | osteoblast / osteoclast / Cbfa1 / BMP / CIZ / signal / bone marrow / progenitor / プロモーター / 転写 / 細胞接着 / 骨再生 / 骨髄幹細胞 / Cbfa / 転写因子 / プロモーター解析 / 軟骨細胞 |
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| Research Abstract |
In this study, we investigated Cbfa1 promoter fragment located up to 1.8 kb up stream to the transcription start site is responsible for the activity of this gene in osteoblastic cells. Further analysis of the 1.8 kb Cbfa1 gene promoter indicated that CIZ can activate Cbfa1 promoter by itself. In addition, in the presence of high levels of BMP signal, Cbfa1 promoter activity is inhibited by the presence of CIZ. Signal interaction analysis indicated that BMP activated Smad action is inhibited by CIZ based on the levels of promoter activations. These data indicated that Cbfa1 is under the control of not only BMP signaling but also by the cell attachment related transcription factor, CIZ. Analysis of the cell transfer experiments using that cells expressing CIZ and Cbfa1 indicated that bone marrow drived progenitor cells could regulate bone marrow and part of the articular catilage.
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