| Project/Area Number |
12557130
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Osaka University |
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Principal Investigator |
MASHIMO Takashi Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (60157188)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBATA Masahiko Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (50216016)
KIYONO Hiroshi Osaka University, Research Institute for Microbial Deseases, Professor, 微生物病研究所, 教授 (10271032)
清水 唯男 大阪大学, 医学系研究科, 助手 (40303961)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 2000: ¥4,900,000 (Direct Cost: ¥4,900,000)
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| Keywords | NF-κB / decoy / heuropathic pain / cytokine / HVJ-liposome / 遺伝子導入 / ベクター / デコイ / HVJ-liposome / 疼痛過敏 |
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| Research Abstract |
Pro-inflammatory cytokines have been shown to be involved in the genesis, persistence, and severity of neuropathic pain following nerve injury. The transcription factor, nuclear factor-kappa B (NF-κB), palys a pivotal role in regulating pro-inflammatory cytokine gene expression. To elucidate the role of NF-κB in the pathogenesis of neuropathic pain, using a gene-based approach of NF-κB decoy, we tested whether the activated NF-κB affected pain behavior via the expression of inflammatory mediators. Single endoneurial injections' of, NF-κB decoy, at the site of nerve lesion, significantly alleviated thermal hyperalgesia for up to 2 weeks and suppressed the expression of mRNA of the inflammatory cytokines, iNOS, and adhesion molecules at the site of nerve injury. This finding suggests that a perineural inflammatory cascade, that involves NF-κB, is involved in the pathogenesis of neuropathic pain
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