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Therapy of neuropathic pain by using a gene-based approach of NF-κB decoy

Research Project

Project/Area Number 12557130
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Anesthesiology/Resuscitation studies
Research InstitutionOsaka University

Principal Investigator

MASHIMO Takashi  Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (60157188)

Co-Investigator(Kenkyū-buntansha) SHIBATA Masahiko  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (50216016)
KIYONO Hiroshi  Osaka University, Research Institute for Microbial Deseases, Professor, 微生物病研究所, 教授 (10271032)
清水 唯男  大阪大学, 医学系研究科, 助手 (40303961)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 2000: ¥4,900,000 (Direct Cost: ¥4,900,000)
KeywordsNF-κB / decoy / heuropathic pain / cytokine / HVJ-liposome / 遺伝子導入 / ベクター / デコイ / HVJ-liposome / 疼痛過敏
Research Abstract

Pro-inflammatory cytokines have been shown to be involved in the genesis, persistence, and severity of neuropathic pain following nerve injury. The transcription factor, nuclear factor-kappa B (NF-κB), palys a pivotal role in regulating pro-inflammatory cytokine gene expression. To elucidate the role of NF-κB in the pathogenesis of neuropathic pain, using a gene-based approach of NF-κB decoy, we tested whether the activated NF-κB affected pain behavior via the expression of inflammatory mediators. Single endoneurial injections' of, NF-κB decoy, at the site of nerve lesion, significantly alleviated thermal hyperalgesia for up to 2 weeks and suppressed the expression of mRNA of the inflammatory cytokines, iNOS, and adhesion molecules at the site of nerve injury. This finding suggests that a perineural inflammatory cascade, that involves NF-κB, is involved in the pathogenesis of neuropathic pain

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Sakaue G, Shimaoka M, Fukuoka T, Hiroi T, Inoue T, Hashimoto N, Sakaguchi T, Sawa Y, Morishita R, Kiyono H, Noguchi K, Mashimo T: "NF-κB decoy supresses cytokine expression and thermal hyperalgesia in a rat neuropathic pain model."NeuroReport. 12. 2079-2084 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hashimoto N, Yamanaka H, Fukuoka T, Dai Y, Obata K, Mashimo T, Noguchi K: "Expression of HGF and cMet in the peripheral nervous system of adults rats following sciatic nerve injury."NeuroReport. 12. 1403-1407 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hashimoto N, Yamanaka H, Fukuoka T, Obata K, Mashimo T, Noguchi K.: "Expression of hepatocyte growth factor in primary sensory neurons of adult rats."Mol Brain Res. 97. 83-88 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakaue G, Shimaoka M, Fukuoka T, Hiroi T, Inoue T, Hashimoto N, Sakaguchi T, Sawa Y, Moricita R, Kiyono H, Noguchi K, Mashimo T.: "NF-κB decoy supresses cytokine expression and thermal hyperalgesia in a rat neuropatic pain model"NeuroReport. 12. 2079-2084 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hashimoto N, Yamanaka H, Fukuoka T, Dai Y, Obata K, Mashimo T, Noguchi K.: "Expression of HGF and cMet in the peripheral nervous system of adult rats following sciatic nerve injury"NeuroReport. 12. 1403-1407 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hashimoto N, Yamanaka H, Fukuoka T, Obata K, Mashimo T, Noguchi K.: "Expression of hepatocyte growth factor in primary sensory neurons of adult rats"Mol Brain Res. 97. 83-88 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakaue G, Shimaoka M, Fukuoka T, Hiroi T, Inoue T, Hashimoto N, Sakaguchi T, Sawa Y, Morishita R, Kiyono H, Noguchi K, Mashimo T: "NF-κB decoy supresses cytokine expression andthermal hyperalgesia in a rat neuropathic pain model"NeuroReport. 12. 2079-2084 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sakaue G, Shimaoka M, Fukuoka T, et al.: "NF-kB decoy supresses cytokine expression and thermal hyperalgesia in rat neuropathic pain model"NeuroReport. 12. 2079-2084 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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