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Analysis of molecular mechanism in endometrial cancer development.

Research Project

Project/Area Number 12557138
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Obstetrics and gynecology
Research InstitutionKyushu University

Principal Investigator

KATO Kiyoko  Kyushu University, Medical Institute of Bioregulation Kyushu Univ. Lecturer, 生体防御医学研究所, 講師 (10253527)

Co-Investigator(Kenkyū-buntansha) WAKE Norio  Kyushu University, Medical Institute of Bioregulation Kyushu Univ. Professor, 生体防御医学研究所, 教授 (50158606)
西田 純一  九州大学, 生体防御医学研究所, 助手 (40264113)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2001: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2000: ¥6,900,000 (Direct Cost: ¥6,900,000)
KeywordsRas / ER / tumorigenicity / Senescence / 造腫瘍能 / RAS
Research Abstract

We previously reported that enhanced transcriptional activation of estrogen receptorα(ERα) contributed to [^<12> Val] K-Ras mediated NIH3T3 cell transformation. Functional inactivation of ERα by a dominant negative mutant of ERα (DNER) in the presence of activated K-Ras 4B mutant arrested the cell cycle at G0/G1, subsequently provoking replicative cell senescence, finally abrogating tumorigenic potential. p53-dependent up-regulation of p21 was implicated in this cell senescence induction. Alterations in the MDM2 protein in response to DNER accounted for this p21-mediated cell senescence induction. An oncogenic K-Ras 4B mutant significantly increased MDM2 proteins coprecipitated with p53, and suppressed p53 transcriptional activity. In turn, DNER exerted its function to decrease MDM2 proteins coprecipitated with p53, followed by the stimulation of p53 activity in the presence of the oncogenic K-Ras 4B mutant. In addition, overexpression of wild type ERα in NIH3T3 cells resulted in the significant increase in the MDM2 protein level and the resultant suppression of p53 transcriptional activity. Finally, we demonstrated that c-Jun expression overcame the suppression and resultant enhancement of p21 protein level in response to DNER. The data imply that the ERα-APl pathway activated by oncogenic K-Ras 4B mutant contributes to the NIH3T3 cells' transformation by modulating p53 transcriptional activity through MDM2.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Ueoka Y et al.: "Hepatocyte growth factor modulates motility and invasiveness of ovarian carcinomas via Ras-mediated pathway"Br.J.Cancer. 84,4. 891-899 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Terao Y et al.: "Sodium butyrate induces growth arrest and senescence-like phenotypes in gynecological cancer cells"International Journal of cancer. 94. 257-267 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kato K et al.: "Contribution of estrogen receptora (ERα) to oncogenic K-Ras-mediated NIH3T3 cell transformation and its implication for escape from senescence by modulating the p53 pathway"J.Biol.Chem. 277,13. 11217-11224 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kato K et al.: "Contribution of estrogen receptor α and progesterone receptor-B to oncogenic K-Ras-mediated NIH3T3 cell transformation"Cell and Molecular Biology of Endometrial Carcinoma : Springer-Verlag Tokyo. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ueoka Y et al.: "Hepatocyte growth factor modulates motility and invasiveness of ovarian carcinomas via Ras mediated pathway"Molecular and cellular endocrinology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ueoka Y et al: "Hepatocyte growth factor modulates motility and invasiveness of ovarian carcinomas via Ras-mediated pathway."British Journal of Cancer. 84,4. 891-899 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Terao Y et al: "Sodium butyrate induces growth arrest and senescence-like phenotypes in gynecological cancer cells."International Journal of cancer. 94. 257-267 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kato K et al: "Contribution of estrogen receptor aα (ERα) to oncogenic K-Ras-mediated NIH3T3 cell transformation and its implication for escape from senescence by modulating the p53 pathway."Journal of Biological Chemistry. 277,13. 11217-11224 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ueoka Y et al: "Hepatocyte growth factor modulates motility and invasiveness of ovarian carcinomas via Ras mediated pathway."Molecular and cellular endocrinology. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kato K et al: "Contribution of estrogen receptor α and progesterone receptor-B to oncogenic K-Ras-mediated NIH3T3 cell transformation., Cell and Molecular Biology of Endoraetrial Carcinoma(in press)"Springer-Verlag Tokyo.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kato K et al.: "Contribution of estrogen receptora (ERα) to oncogenic K-Ras-mediated NIH3T3 cell transformation and its implication for escape from senescence by modulating the p53 pathway"J. Biol. Chem. 277,13,. 11217-11224 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kato H et al.: "Growth-associated Gene Expression Profiles by Microarray Analysis of Trophoblast of Molar Pregnancies and Normal Villi"International Journal of Gynecological Pathology. 21,3. 255-260 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Zhou Y et al.: "Identification of FOXC1 as a TGF-β1 responsive gene and its involvement in negative regulation of cell growth"Genomics. 80,5. 465-472 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kato K et al.: "Contribution of estrogen receptor α and progesterone receptor-B to oncogenic K-Ras-mediated NIH3T3 cell transformation"Cell and Molecular Biology of Endometrial Carcinoma : Springer-Verlag Tokyo. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Ueoka Y et al.: "Hepatocyte growth factor modulates motility and invasiveness of ovarian carcinomas via Ras mediated pathway"Molecular and cellular endocrinology. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] N.Shahib et al.: "Genetic Origin of Malignant Trophoblastic Neoplasms Analysed by Sequence Tag Site Polymorphic Markers"Gynecologic Oncology. 81. 247-253 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Terao Y et al.: "Sodium butyrate induces growth arrest and senescence-like phenotypes in gynecological cancer cells"International Journal of cancer. 94. 257-267 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kato K et al.: "Contribution of estrogen receptora (ERa) to oncogenic K-Ras-mediated NIH3T3 cell transformation and its implication for escape from senescence by modulating the p53 pathway"Journal of Biological Chemistry. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] 加藤聖子: "新女性医学大系41(rasとそのシグナル:婦人科腫瘍の分子・細胞生物学)"中山書店. 23 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ueoka Y et al: "Hepatocyte growth factor modulates motility and invasiveness of ovarian carcinomas via Ras-mediated pathway."Br.J.Cancer. 84,4. 891-899 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Murakami A et al: "Photodynamic antisense regulation (PDAR) of human cervical carcinoma cell growth using psoralen-conjugated oligo (nucleoside phosphorothioate)."European Journal of Pharmaceutical Science. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 堀内新司 他: "【特集】2.性ステロイドの効果発現機序に関する最近の話題"産科と婦人科. 67,1. 14-19 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 西田純一 他: "【治療トピック6-新たな治療への展開2】遺伝子治療"臨床婦人科産科. 54,6. 819-822 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 加藤聖子: "子宮内膜の増殖・分化に関与する情報伝達系"日本産科婦人科学会雑誌. 52,8. 1162-1170 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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