| Project/Area Number |
12557149
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Ophthalmology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
KINOSHITA Shigeru Medicine, Ophthalmology, Kyoto Prefectural University of Medicine Professor, 医学部, 教授 (30116024)
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| Co-Investigator(Kenkyū-buntansha) |
SHINOHARA Chikara Japan Tissue Engineering Research cluster, ティッシュ・エンジニアリング・研究開発部, 培養研究クラスター
HONJO Hideo Medicine, Obstetrics and Gynecology, Kyoto Prefectural University of Medicine Professor, 医学部, 教授 (30110852)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2001: ¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 2000: ¥6,800,000 (Direct Cost: ¥6,800,000)
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| Keywords | amnion / culture / corneal epithelium / stem cell / ocular surface disease / ocular surface reconstruction / スラムセル |
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| Research Abstract |
In severe ocular surface diseases, such as Stevens-Johnson syndrome and ocular cicatricial pemphigoid, in which I limbal epithelial cells are destroyed, the neighboring conjunctival epithelial cells invariably cover the corneal surface resulting in chronic inflammation, stromal scarring, neovascularization which severely affects visual acuity. In such diseases, because the prognosis after penetrating keratoplasty is poor, the alternative surgical treatment of corneal epithelial transplantation (limbal transplantation or keratoepithelioplasty) has been developed to improve the outcome of ocular surface reconstruction. The most recently developed treatment for these diseases involves the use of cultivated corneal epithelial stem cell transplantation for reconstructing the ocular surface damage caused by corneal epithelial stem cell deficiencies. Our group developed a corneal limbal epithelial culture system, using AM as a carrier, in an animal and human model. We further have successfully regenerated corneal epithelial cells in vitro using a cell suspension culture system. The cultivated cells showed 4-5 layers of stratification, were well differentiated, and stained with cornea-specific keratin 3 /12 and cluster in. We have since adopted this system for clinical use in severe ocular surface disorders and have successfully achieved ocular surface reconstruction. In all cases, the corneal surfaces were clear, smooth and were perfectly covered with transplanted allo-corneal epithelium at 48 hours after the first transplantation.
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