| Project/Area Number |
12557156
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 展開研究 |
|---|
| Research Field |
Functional basic dentistry
|
|---|
| Research Institution | HIROSHIMA UNIVERSITY |
|---|
Principal Investigator |
KATO Yukio Hiroshima University Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (10112062)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAWAMOTO Takeshi Hiroshima University Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (50224861)
NOSHIRO Mitsuhide Hiroshima University Graduate School of Biomedical Sciences, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (00144858)
申 鳴 広島大学, 歯学部, 教務員 (20294572)
|
|---|
| Project Period (FY) |
2000 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 2002: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2000: ¥4,800,000 (Direct Cost: ¥4,800,000)
|
|---|
| Keywords | transcription / cartilage / mesenchymal stem cells / regeneration / DEC1 / MTF |
|---|
| Research Abstract |
We succeeded in regeneration of cartilage and/or bone defects after transplantation of bone marrow mesenchymal stem cells (MSC) in animal models. In addition, forced expression of DEC1 gene in human MSC stimulated chondrocyte differentiation in vitro, DEC1 forced expression suppressed myogenic and adipogenic differentiation and enhanced chondrogenic differentiation. Furthermore, foiled expression of membrane-bound transferrin-like protein (MTF) in mesenchymal cells also enhanced chondrogenic differentiation. Various growth factors and hypoxia stimulated the expression of MTF, DEC1 and/or DEC2 in MSC cultures. These findings suggest that MSC transfected with DEC1, DEC2 or MTF are useful for regeneration medicine.
|
