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Analysis of genetic risk factors in periodontal diseses ― Effect of cellular aging on damage of cells ―

Research Project

Project/Area Number 12557178
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 矯正・小児・社会系歯学
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

HASEGAWA Tomokazu  Hokkaido Univ., Grad. School of Dent., Inst., 大学院・歯学研究科, 助手 (50274668)

Co-Investigator(Kenkyū-buntansha) KIKUIRI Takashi  Hokkaido Univ., Grad. School of Dent., Inst., 大学院・歯学研究科, 助手 (10322819)
SAITOH Akira  Hokkaido Univ., Grad. School of Dent., Inst., 大学院・歯学研究科, 助手 (20301913)
SHIRAKAWA Tetsuo  Hokkaido Univ., Dent. Hospital, Lec., 歯学部・附属病院, 講師 (00187527)
FUKUMOTO Satoshi  Nagasaki Univ., School of Dent., Inst., 歯学部, 助手 (30264253)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥10,700,000 (Direct Cost: ¥10,700,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2000: ¥8,600,000 (Direct Cost: ¥8,600,000)
Keywordsgenetic risk factor / periodontal disease / Down syndrome / telomere DNA length / cellular aging / Interferon receptor / STAT1 / Interleukin-6 / 先天性リスクファクター / STA1 / 特異的発現遺伝子 / DNA chip / Microarray
Research Abstract

1. Telomere DNA length of human fibroblasts derived from healthy and Down syndrome patients was analyzed by Southern blotting. After 5 times cell replication, it was shown that telomere DNA length was more short in fibroblasts from Down syndrome than from healty fibroblasts.
2. Fibroblasts derived from Down syndrome patients expressed interferon alpha/ beta (IMF-α/β) receptor subunit, IFNAR1, stronger than fibroblasts from healthy patients.
3. STAT1, one of transcriptional factors in the downstream of IMF receptor, was activated by the treatment of INF- γ. Western blotting demonstrated that activated STAT1 in Down fibroblasts was expressed stronger than in healthy fibroblasts after the treatment of INF-γ. However, in the presence of anti-INF-β antibody, activated STAT1 was reduced by the treatment of INF-γ.
4. Interleukin-6 (IL-6), one of inflammatory cytokines, was induced by the treatment of INF -γ. ELISA demonstrated that IL-6 production in Down fibroblasts increased more abundantly than in healthy fibroblasts by the treatment of INF- γ.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Hasegawa, T.: "Human periodontal ligament cells derived from deciduous teeth induce osteoclastogenesis in vitro"Tissue and Cell. 34(in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hasegawa, T.: "Expression of osteoprotegerin/osteoclastogenesis inhibitory factor and osteoprotegerin ligand/osteoclast differentiation factor in cultures of periodontal ligament cells derived from human permanent teeth"Journal of Periodontal Research. 37(in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hasegawa, T. (et al.): "Human periodontal ligament ells derived from deciduous teeth induce osteoclastogenesis In vitro"Tissue and Cell. 34 (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hasegawa, T. (et al.): "Expression of osteoprotegerin/osteoclastogenesis inhibitory factor and osteoprotegerin ligand/osteoclast differentiation factor in cultures of periodontal ligament cells derived from human permanent teeth"J. Periodontal Research. 37 (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hasegawa, T.: "Human periodontal ligament cells derived from deciduous teeth induce osteoclastogenesis in vitro"Tissue and Cell. 34(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hasegawa, T.: "Expression of osteoprotegerin/osteoclastogenesis inhibitory factor and osteoprotegerin ligand/osteoclast differentiation factor in cultures of periodontal ligament cells derived from human permanent teeth"Journal of Periodontal Research. 37(in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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