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RESEARCH OF PERIODONTAL TISSUE REMODELING ACTIVATION INDUCED BY VIBRATION AND ITS CLINICAL APPLICATION

Research Project

Project/Area Number 12557179
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 矯正・小児・社会系歯学
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

CHIBA Mirei (2001-2003)  TOHOKU UNIVERSITY, GRADUATE SCHOOL OF DENTISTRY, RESEARCH ASSOCIATE, 大学院・歯学研究科, 助手 (10236820)

清水 義信 (2000)  東北大学, 大学院・歯学研究科, 助教授 (20005078)

Co-Investigator(Kenkyū-buntansha) KAGAYAMA Manabu  TOHOKU UNIVERSITY, GRADUATE SCHOOL OF DENTISTRY, PROFESSOR, 大学院・歯学研究科, 教授 (60004610)
SHIMIZU Yoshiyuki  TOHOKU UNIVERSITY, HOSPITAL, LECTURER, 医学部附属病院, 講師 (20187470)
SHIMIZU Yoshinobu  TOHOKU UNIVERSITY, GRADUATE SCHOOL OF DENTISTRY, ASSOCIATE PROFESSOR, 大学院・歯学研究科, 助教授 (20005078)
MITANI Hideo  TOHOKU UNIVERSITY, GRADUATE SCHOOL OF DENTISTRY, PROFESSOR, 大学院・歯学研究科, 教授 (50014220)
三木 美麗  東北大学, 大学院・歯学研究科, 助手 (10236820)
Project Period (FY) 2000 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2000: ¥6,800,000 (Direct Cost: ¥6,800,000)
KeywordsResonance Vibration / Orthodontic Tooth Movement / Mechanical Stress / Periodontal tissue / RANKL / OPG / Osteoclasts / Root Resorption / 振動刺激 / 共振周波数 / 歯周組織改造機構 / 歯科矯正学的歯の移動 / 共振点 / 歯周組織改造機 / プロスタグランジン / 歯周組織再生 / 共焦点 / プロタグランジン
Research Abstract

Objectives:
Accelerating the speed of orthodontic tooth movement should shorten the treatment period, which is one of the negative aspects of orthodontic treatment. This study evaluated the effects of mechanical stimulation using resonance vibration on tooth movement and revealed the cellular and molecular mechanism of the periodontal ligament responses.
Methods:
The upper first molars of 6-week-old male Wistar rats were moved to the buccal side using an expansive spring for 21 days (n=6,control group) and the amount of tooth movement was measured. Additional vibrational stimulation (60 Hz,1.0 m/s^2) was applied to rat first molars using a loading vibration system for 8 minutes on days 0,7,and 14 during orthodontic tooth movement (n=6,experimental group). The animals were sacrificed under anesthesia and the upper jaw dissected. The specimens were fixed, decalcified, and embedded in paraffin. Sections were used for immunohistochemical analysis of receptor activator of NF kappa B ligand (RANKL) expression. The number of osteoclasts in the alveolar bone was counted using TRAP staining and the amount of root resorption was measured in sections stained with H-E.
Results:
The average resonance frequency of the upper first molar was 61.02±8.38 Hz. Tooth movement in the experimental group was significantly greater than in the control group (p<0.05). Enhanced RANKL expression was observed at fibroblasts and osteoclasts in the periodontal ligament of the experimental group on day 3. The number of osteoclasts in the experimental group was significantly increased over the control group on day 8 (p<0.05). Histologically, there were no pathological findings in either group or significant differences in the amount of root resorption between the two groups.
Conclusion:
The application of resonance vibration might accelerate orthodontic tooth movement via enhanced RANKL expression in the periodontal ligament without additional damage to periodontal tissues, such as root resorption.

Report

(5 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (94 results)

All Other

All Publications (94 results)

  • [Publications] Okazaki, M., Shimizu, Y., Chiba.M., Mitani, H.: "Expression of heat shock proteins induced by cyclical stretching stress in human periodontal ligament fibroblasts."Tohoku University Dental Journal. 19. 108-115 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chiba, M., Yamaguchi N., Igarashi K., Kanzaki H., Yamada S., Mitani: "Mechanical stress induced both NO and PGE2 in PDL cells"Journal of Dental Research. 791. 145 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kanzaki, H., Chiba M., Shimizu Y., Mitani H.: "ODF and OCIF mRNA expressed by mechanical stress"Journal of Dental Research. 79. 590 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Totsuka M., Chiba M., Mitani H.: "The effect of mechanical stretching on skeletal muscle organogenesis in vitro"Japanese Journal of Oral Biology. 42. 233-244 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kubota M., Chiba M., Kanzaki H., Mitani H.: "Establishment of immortalized periodontal ligament cells derived from oncogene transgenic rat."Japanese Journal of Oral Biology. 42. 590 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chiba M., Tsuchiva S., Kanzaki H., Kubota M., Mitani H.: "Establishment of the gene transfer technique by in vivo elevtroporation or adenovirus vector.-Basic study for the gene therapy of periodontal disease."Japanese Journal of Oral Biology. 43. 540 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Kubota, M.Chiba, H.Kanzaki, N.Yanai, M.Obinata, S.Ueda, H.Mitani: "Establishment of Immortalized Periodontal ligament cell lines and gene expression"Journal of Dental Research. 80. 585 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] N.Yamaguchi, M.Chiba, H.Mitani: "Mechanical stress induces c-fos mRNA expression in human PDL-fibroblasts."Journal of Dental Research. 80. 585 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kanzaki, H., Chiba, M., Shimizu, Y., Mitani, H.: "Dual regulation of osteoclast differentiation by periodontal ligament cells through ODF stimulation and OPG inhibition."Journal of Dental Research. 80. 887-891 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kanzaki, H., Chiba, M., Shimizu, Y., Mitani, H.: "Periodontal Ligament Cells Under Mechanical stress Induce Osteoclastogenesis by Receptor Activator of Nuclear Factor κB Ligand Up-Regulation via Prostaglandin E2 Synthesis."Journal of Bone and Mineral Research. 17. 210-220 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaguchi, N., Chiba, M., Mitani, H.: "The induction of c-fos mRNA expression by mechanical stress in human periodontal ligament cells"Archives of Oral Biology. 47. 465-471 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kanzaki, H., Chiba, M., Nishimura, M., Mitani, H.: "RANKL Upregulation in the Compressed Area of Periodontal Ligament induces Orthodontic Tooth Movement"Journal of Dental Research. 81. A-135 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsumoto, C., Chiba, M., Kanzaki H., Kubota, M., Mitani, H.: "Synergistic human osteoclast differentiation inducing by the combined use of 1,25(OH)2D 3, RANKL, and M-CSF"Journal of Dental Research. 81. A-105 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishimura, M., Chiba, M., Kanzaki, H., Mitani, H.: "The Expression of Osteoprotegerin in the Periodontal Ligament in Experimental Tooth Movement"Journal of Dental Research. 81. A-135 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsuchiya, S., Chiba, M., Kishimoto, K., Nakamura, H., Mitani, H.: "Gene transfer into periodontal tissue by in vivo electroporation"Journal of Dental Research. 81. A-452 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chiba, M.: "Molecular biological review on bone remodeling based on mechanical stress."the Quintessence. 21. 931-938 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chiba, M., Kubota, M., Obinata, M., Mitani, H.: "Establishing Immortalized Periodontal Ligament Cells from Oncogene Transgenic Rat and Gene Expression of Bone Metabolic Markers"Journal of bone and mineral research. 17. S223 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chiba, _M., Nukatsuka, S., Kanzaki, H., Matsumoto, C., Mitani, H.: "Mechanical tension force inhibits osteoclastogenesis via osteoprotegerin production from periodontal ligament cells"Bone. 32. S197 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Goga, Y., Shimizu Y., Chiba M., Mitani H.: "Continuous compressive force induces apoptosis in human osteoblast-like cells."Bone. 32. S138 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nukatsuka, S., Chiba, M., Kanzaki, H., Mitani, H.: "Effect of mechanical stretching on the regulatory mechanism of bone resorption in human periodontal ligament cells."Japanese Journal of Oral Biology. 44. 113 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kanzaki, H., Chiba, M., Takahashi, I., Nishimura, M., Haruyama, N., Nishimura M., Mitani, H.: "Local osteoprotegerin (OPG) gene transfer to the periodontium inhibits orthodontic tooth movement."Journal of Dental Research. 82. B-371 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishimura, M., Chiba, M., Haruyama, N., Igarashi, K., Mitani, H.: "Effects of resonance vibration during experimental tooth movement in rats."Journal of Dental Research. 83. A-2603 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kanzaki.H., Chiba, M., Takahashi, I., Haruyama, N., Nishimura, M., Igarashi, K., Mitani, H.: "Local RANKL-gene Transfer to the Periodontium Accelerates Orthodontic Tooth Movement."Journal of Dental Research. 83. A-2999 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Goga, Y., Chiba M., Shimizu Y., Mitani H.: "Continuous Compressive Force induces Osteoblast Apoptosis via caspase-8 activation."Journal of Dental Research. 83. A-2597 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsumoto C., Chiba.M., Kanzaki, H., Igarashi K., Mitani, H.: "The Molecular Mechanism of Human Osteoclastogenesis in HL-60 Cells"Journal of Dental Research. 83. A-2198 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chiba, M., Mitani, H.: "Cytoskeletal changes and the system of regulation of alkaline phosphatase activity in human periodontal ligament cells induced by mechanical stress"Cell Biochemistry and Function. (in press)(Published online, Feb 2004). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kubota, M., Chiba, M., Obinata, M., Ueda, S., Mitani, H.: "Establishment of periodontal ligament cells from temperature-sensitive simian virus 40 large T-antigen transgenic rats"Cytotechnology. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okazaki, M., Shimizu, Y., Chiba, M., Mitani, H.: "Expression of heat shock proteins induced by cyclical stretching stress in human periodontal ligament fibroblasts."Tohoku University Dental Journal. 19. 108-115 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chiba, M., Yamaguchi N., Igarashi K., Kanzaki H., Yamada S., Mitani: "Mechanical stress induced both NO and PGE2 in PDL cells"Journal of Dental Research. 791. 145 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kanzaki, H., Chiba M., Shimizu Y., Mitani H.: "ODF and OCIF mRNA expressed by mechanical stress"Journal of Dental Research. 79. 590 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Totsuka M., Chiba M., Mitani H.: "The effect of mechanical stretching on skeletal muscle organogenesis in vitro"Japanese Journal of Oral Biology. 42. 233-244 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kubota M., Chiba M., Kanzaki H., Mitani H.: "Establishment of immortalized periodontal ligament cells derived from oncogene transgenic rat."Japanese Journal of Oral Biology. 42. 590 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chiba M., Tsuchiya S., Kanzaki H., Kubota M., Mitani H.: "Establishment of the gene transfer technique by in vivo elevtroporation or adenovirus vector.-Basic study for the gene therapy of periodontal disease."Japanese Journal of Oral Biology. 43. 540 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Kabota, M.Chiba, H.Kanzaki, N.Yanai, M.Obinata, S.Ueda, H.Mitani: "Establishment of Immortalized Periodontal ligament cell lines and gene expression"Journal of Dental Research. 80. 585 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] N.Yamaguchi, M.Chiba, H.Mitani: "Mechanical stress induces c-fos mRNA expression in human PDL-fibroblasts."Journal of Dental Research. 80. 585 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kanzaki, H., Chiba, M., Shimizu, Y., Mitani, H.: "Dual regulation of osteoclast differentiation by periodontal ligament cells through ODF stimulation and OPG inhibition."Journal of Dental Research. 80. 887-891 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kanzaki, H., Chiba, M., Shimizu, Y., Mitani, H.: "Periodontal Ligament Cells Under Mechanical stress Induce Osteoclastogenesis by Receptor Activator of Nuclear Factor κB Ligand Up-Regulation via Prostaglandin E2 Synthesis."Journal of Bone and Mineral Research. 17. 210-220 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaguchi, N., Chiba, M., Mitani, H.: "The induction of c-fos mRNA expression by mechanical stress in human periodontal ligament cells"Archives of Oral Biology. 47. 465-471 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kanzaki, H., Chiba, M., Nishimura, M., Mitani, H.: "RANKL Upregulation in the Compressed Area of Periodontal Ligament induces Orthodontic Tooth Movement"Journal of Dental Research. 81. A-135 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsumoto, C., Chiba, M., Kanzaki H., Kubota, M., Mitani, H.: "Synergistic human osteoclast differentiation inducing by the combined use of 1,25(OH)2D 3,RANKL, and M-CSF"Journal of Dental Research. 81. A-105 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishimura, M., Chiba, M., Kanzaki, H., Mitani, H.: "The Expression of Osteoprotegerin in the Periodontal Ligament in Experimental Tooth Movement"Journal of Dental Research. 81. A-135 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsuchiya, S., Chiba, M., Kishimoto, K., Nakamura, H., Mitani, H.: "Gene transfer into periodontal tissue by in vivo electroporation"Journal of Dental Research. 81. A-452 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chiba, M.: "Molecular biological review on bone remodeling based on mechanical stress."the Quintessence. 21. 931-938 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chiba, M., Kubota, M., Obinata, M., Mitani, H.: "Establishing Immortalized Periodontal Ligament Cells from Oncogene Transgenic Rat and Gene Expression of Bone Metabolic"Journal of bone and mineral research. 17. S223 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chiba, M., Nukatsuka, S., Kanzaki, H., Matsumoto, C., Mitani, H.: "Mechanical tension force inhibits osteoclastogenesis via osteoprotegerin production from periodontal ligament cells"Bone. 32. S197 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Goga, Y., Shimizu Y., Chiba M., Mitani H.: "Continuous compressive force induces apoptosis in human osteoblast-like cells."Bone. 32. S138 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nukatsuka, S., Chiba, M., Kanzaki, H., Mitani, H.: "Effect of mechanical stretching on the regulatory mechanism of bone resorption in human periodontal ligament cells."Japanese Journal of Oral Biology. 44. 113 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kanzaki, H., Chiba, M., Takahashi, I., Nishimura, M., Haruyama, N., Nishimura M., Mitani, H.: "Local osteoprotegerin (OPG) gene transfer to the periodontium inhibits orthodontic tooth movement."Journal of Dental Research. 82. B-371 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishimura, M., Chiba, M., Haruyama, N., Igarashi, K., Mitani, H.: "Effects of resonance vibration during experimental tooth movement in rats."Journal of Dental Research. 83. A-2603 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kanzaki, H., Chiba, M., Takahashi, I., Haruyama, N., Nishimura, M., Igarashi, K., Mitani H.: "Local RANKL-gene Transfer to the Periodontium Accelerates Orthodontic Tooth Movement."Journal of Dental Research. 83. A-2999 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Goga, Y., Chiba M., Shimizu Y., Mitani H.: "Continuous Compressive Force induces Osteoblast Apoptosis via caspase-8 activation."Journal of Dental Research. 83. A-2597 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsumoto C., Chiba, M., Kanzaki, H., Igarashi K., Mitani, H.: "The Molecular Mechanism of Human Osteoclastogenesis in HL-60"Journal of Dental Research. 83. A-2198 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chiba, M., Mitani, H.: "Cytoskeletal changes and the system of regulation of alkaline phosphatase activity in human periodontal ligament cells induced by mechanical stress"Cell Biochemistry and Function (Published online, Feb 2004). (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kubota, M., Chiba, M., Obinata, M., Ueda, S., Mitani, H.: "Establishment of periodontal ligament cells from temperature-sensitive simian virus 40 large T-antigen transgenic rats"Cytotechnology. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chiba Mirei: "Mechanical tension force inhibits osteoclastogenesis via osteoprotegerin production from periodontal ligament cells"Bone. 32. S197 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Goga Y.: "Continuous compressive force induces apoptosis in human osteoblast-like cells"Bone. 32. S138 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kanzaki H.: "Local osteoprotegerin (OPG) gene transfer to the periodontium inhibits Orthodontic tooth movement"Journal of Dental Research. 82. B-371 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 千葉 美麗: "Adenovirus vector, HVJ-envelope vectorおよびin vivo electroporationを用いた歯周組織への遺伝子導入"炎症再生. 23. 511 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 菅崎 弘幸: "HVJ envelp vectorによるOPG遺伝子導入を用いた新しい歯槽骨骨代謝制御機構の開発"炎症再生. 23. 511 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.NISHIMURA: "Effects of Resonance Vibration During Experimental Tooth Movement in Rats"Journal of Dental Research. 83(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Goga: "Continuous Compressive Force induces Osteoblast Apoptosis via caspase-8 activation"Journal of Dental Research. 83(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] C.MATSUMOTO: "The Molecular Mechanism of Human Osteoclastgenesis in HL-60 Cells"Journal of Dental Research. 83(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.KANZAKI: "Local RANKL-gene Transfer to the Periodontium Accelerates Orthodontic Tooth Movement"Journal of Dental Research. 83(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.KANZAKI: "RANKL Upregulation in the Compressed Area of Periodontal Ligament induces Orthodontic Tooth Movement"Journal of Dental Research. 81. A-135 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] C.MATSUMOTO: "Synergistic human osteoclast differentiation inducing by the combined use of 1,25(OH)2D 3, RANKL, and M-CSF"Journal of Dental Research. 81. A-105 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.NISHIMURA: "The Expression of Osteoproteger in in the Periodontal Ligament in Experimental Tooth Movement"Journal of Dental Research. 81. A-135 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.TSUCHIYA: "Gene transfer into periodontal tissue by in vivo electroporation"Journal of Dental Research. 81. A-452 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kanzaki, H: "Periodontal Ligament Cells Under Mechanical stress Induce Osteoclastogenesis by Receptor Activatior of Nuclear Factor κ B Ligand Up-Regulation via Prostaglandin E2 Synthesis"Journal of Bone and Mineral Research. vol.17, No.2. 210-220 (2002)

    • Related Report
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  • [Publications] Yamaguchi N: "The induction of c-fos mRNA expression by mechanical stress in human periodontal ligament cells"Archives of Oral Biology. 47. 465-471 (2002)

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  • [Publications] M.CHIBA: "Establishing Immortalized Periodontal Ligament Cells from Oncogene Transgenic Rat and Gene Expression of Bone Metabolic Markers"Journal of Bone and Mineral Research. 17. S223 (2002)

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  • [Publications] 糠塚世毅: "ヒト歯根膜線維芽細胞の骨吸収制御機構に対する機械的伸展力の影響"歯科基礎医学会雑誌. 44巻5号. 113 (2002)

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  • [Publications] 千葉美麗: 日本実験力学学会第2回バイオメカニクス分科会講演論文集. No.2-1. 44-47 (2002)

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  • [Publications] M.CHIBA: "Molecular biological review on bone remodeling based on mechanical stress"the Quintessence. 21. 931-938 (2002)

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  • [Publications] 千葉美麗: "メカニカルストレスによる歯根膜細胞の初期応答"日本機会学会 第13回バイオエンジニアリング講演会講演論文集. No.00-35. 192-193 (2000)

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  • [Publications] M.Kubota: "Estblishment of Immortalized Periodontal ligament cell lines and gene expression"Journal of Dental Research. 80. 585 (2001)

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  • [Publications] 山口希実: "メカニカルストレスによる歯根膜線維芽細胞の転写因子c-fosの発現誘導"日本実験力学学会 第一回研究発表講演会講演論文集. No.1. 83-86 (2001)

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  • [Publications] 千葉美麗: "1,25(OH)2D3, RANLL, M-CSF併用によるstage-specificなヒト破骨細胞分化誘導相乗作用"日本骨代謝学会誌. 19巻2号. 99 (2001)

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  • [Publications] 千葉美麗: "In vivo electroporationやadenovirus vectorを用いた歯周組織への遺伝子導入法の確立-歯周疾患遺伝子治療法の基礎的検討-"歯科基礎医学会誌. 43巻5号. 540 (2001)

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  • [Publications] H.KANZAKI: "RANKL Upregulation in the Compressed Area of Periodontal Ligament induces Orthodontic Tooth Movement"Journal of Dental Research. 81(in press). (2002)

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  • [Publications] C.MATSUMOTO: "Synergistic human osteoclast differentiation inducing by the combined use of 1,25(OH)2D 3, RANK, and M-CSF"Journal of Dental Research. 81(in press). (2002)

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  • [Publications] M.NISHIMURA: "The Expression of Osteoprotegerin in the Periodontal Ligament in Experimental Tooth Movement"Journal of Dental Research. 81(in press). (2002)

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  • [Publications] S.TSUCHIYA: "Gene transfer into periodontal tissue bi in vivo electroporationon"Journal of Dental Research. 81(in press). (2002)

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  • [Publications] Kanzaki: "Dual regulation of osteoclast differentiation by periodontal ligament cells through ODF stimulation and OPG inhibition"Journal of Dental Research. vol.80,No.3. 887-891 (2001)

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  • [Publications] Kanzaki, H: "Periodontal Ligament Cells Under Mechanical stress Induce Osteoclastogenesis by Receptor Activatior of Nuclear FactorκB Ligand Up-Regulation via Prostaglandin E2 Synthesis"Journal of Bone and Mineral Research. vol.17,No.2. 210-220 (2002)

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  • [Publications] Yamaguchi N: "Mechanical stress induces c-fos mRNA expression in human periodontal ligament cells"Archives of Oral Biology. (in press). (2002)

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  • [Publications] 千葉美麗: "メカニカルストレスによる歯根膜細胞の初期応答"日本機械学会[No.00-35]第13回バイオエンジニアリング講演会講演論文集. 192-193 (2001)

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  • [Publications] 戸塚実: "骨格筋細胞に対する機械的伸展刺激の影響に関する研究"歯科基礎医学会雑誌. 42・3. 233-244 (2000)

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  • [Publications] Chiba,M.: "Mechanical stress induced both NO and PGE2 in PDL cells."Journal of Dental Research. 79・suppl. 145 (2000)

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  • [Publications] Kanzaki,H.: "ODF and OCIF mRNA expressed by mechanical stress."Journal of Dental Research. 79・suppl. 590 (2000)

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  • [Publications] 菅崎弘幸: "メカニカルストレスによる歯根膜線維芽細胞のODF mRNA発現増強とPGE2の関与"日本骨代謝学会雑誌. 18・2. 200 (2000)

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  • [Publications] 久保田衛: "Oncogene transgenic ratを用いた歯根膜不死化細胞の樹立"歯科基礎医学会雑誌. 42・5. 590 (2000)

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  • [Publications] Kanzaki,H.: "Dual regulation of osteoclast differentiation by periodontal ligament cells through RANKL stimulation and OPG inhibition."Journal of Dental Research. 80(In press). (2001)

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  • [Publications] Kubota,M.: "Establishment of immortalized periodontal ligament cell lines and gene expression."Journal of Dental Research. 80・suppl(In press). (2001)

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  • [Publications] Yamaguchi,M.: "Mechanical stress induces c-fos mRNA expression in human PDL-fibroblasts."Journal of Dental Research. 80・suppl(In press). (2001)

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Published: 2000-04-01   Modified: 2016-04-21  

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