| Project/Area Number |
12557190
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Tokyo Medical and Denial University |
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Principal Investigator |
ISHIKAWA Isao. Tokyo Mod. & Dent. Univ., Professor, 大学院・医歯学総合研究科, 教授 (10014151)
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| Co-Investigator(Kenkyū-buntansha) |
AOKI Akira Tokyo Med. & Dent. Uuiv., Instructor, 大学院・医歯学総合研究科, 助手 (30302889)
KINOSHITA Atsuhiro Tokyo Med. & Dent. Univ., Instructor, 大学院・医歯学総合研究科, 助手 (10242207)
ODA Shigeru Tokyo Med. & Dent. Univ., Lecturer, 大学院・医歯学総合研究科, 講師 (70160869)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2000: ¥6,800,000 (Direct Cost: ¥6,800,000)
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| Keywords | BMP / Bone Morphogenetic Protein / Enaniel Matrix Derivative / EMD / Regenerative Therapy / Periodontal Regeneration / Periodontal Ligament / BMD |
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| Research Abstract |
Many reports have indicated that recombinant human bone morphogenetic protein-2 (rhBMP-2) induced bone formatiou in vivo, however, it is unkown whether rhBMP-2 is effective to regenerate the cementum.
We performed experiments to clarify whether the application of rhBMP-2 induces new cementum formation in the conditions with inhibition of periodontal ligament (PDL) cell migration and whether the inhibition of PDL cell migration L is influence on the new bone formation after rhBMP-2 applicaliou in beagle dogs.
The results indicate that rhBMP-2/PGS complex (0.4mg/ml) does not induce any new cementum when the coronal migration of cells from intact PDL tissue is inhibited, and that the amount of new bone formed with rhBMP-2/PGS complex is not influenced by the inhibition of PDL cell migration.
We performed experiments to evaluate the periodontal wound healing alter the application of coarnel matrix derivative (EMD) in rats. Newly formed calcified tissues were observed qualitatively and quantitatively. The results suggest that EMD has a potential to promote the cementum regeneration especially fiber inserted cementum and to create a favorable environment that will promote the periodontal regeneration.
We performed experiments to evaluate the effects of combined application of rhBMP-2 and EMD in beagle dogs. EMD was applied only in the experimental site and rhBMP-2/PGS complex (0.4mg/ml) was applied in the experimental and control sites. Considerable amounts of new bone formation were observed in all groups regardless of the application of EMD. The distance between the bottom of the defect and apical terminal of ankylosis was significantly greater in the group with EMD application. The results indicate that cell migration from intact PDL tissue is essential for the formation of new PDL tissue even with the combined application of rhBMP-2 and EMD, and that the EMD application seems to enhance the connective tissue attachment.
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