| Project/Area Number |
12557195
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
SAITO Masahiro Kanagawa Dental College, Department of Operative Dentistry and Endodontics, Assistant professor, 歯学部, 講師 (40215562)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAUCHI Masato Kanagawa Dental College, Department of Orthodontics, Assistant professor, 歯学部, 助手 (30230311)
TSUNODA Akira Kanagawa Dental College, Department of Operative Dentistry and Endodontics, Assistant professor, 歯学部, 助手 (70236933)
SUZUKI Motoshi Nagoya University School of Medicine, Laboratory of Cancer Cell Biology, Research Institute for Disease Mechanism and Control, Lecturer, 医学部, 講師 (80236017)
SASAKURI Kenichi Kanagawa Dental College, Department of Orthodontics, Assistant professor, 歯学部, 助手 (10235286)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2000: ¥11,200,000 (Direct Cost: ¥11,200,000)
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| Keywords | Regeneration / Periodontium / Bone Sialoprotein / Baculovirus Expression System / Recombinant Protein / Cementum / Development / Regenerative Therapy |
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| Research Abstract |
Cementum is the calcified outer layer through which collagen fibers of connective tissue are anchored into tooth root surfaces. The attachment of connective tissue to root surface is compromised when the cementum is affected by periodontitis, a chronic inflammatory disease, and this can result in irreversible damage to the periodontium. Clinically, the regeneration of cementum is crucial to restore connective tissue attachment because diseased root surface cannot promote attachment and migration of periodontal ligament cells. However, despite many novel approaches available for this purpose, so far it has not been possible to predictably form new cementum. To develop effective material for periodontal regeneration, cementum matrix protein is necessary to recruit the cells which are essential for development and formation of cementum and periodontal ligament and influence their biological activities. Bone sialoprotein is a phosphorylated glycoprotein found in the cementum matrix. During
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cementogenesis, it is expressed in cementoblasts, and interaction between the cementoblasts and BSP may be critical for cementum formation. With the recent development of recombinant DNA technology, human protein that were normally available due to exceedingly small amount being able to produce for basic research as well as clinical application. In order to improve the regenerative technologies of periodontium, we produced recombinant human BSP (rhBSP). rhBSP with his_6 fusion peptide was expressed in insect cells using a baculovirus expression system, and purified by metal-affinity column chromatography. Purified rhBSP migrated as discrete bands of 60 kDa and 65 kDa in an SDS PAGE and Western blotting. Bovine dental follicle cells, which contain cementoblast progenitors, adhered to the dish surface when it was coated by rhBSP. These results indicate that the baculovirus expression system is useful in producing functional rhBSP, and that the rhBSP may develop the novel regenerative technologies of periodontium. Less
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