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Development of artificial periodontal membranes for periodontal disease using polyphosphate

Research Project

Project/Area Number 12558099
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biomedical engineering/Biological material science
Research InstitutionMatsumoto Dental University (2002-2003)
Hokkaido University (2000-2001)

Principal Investigator

YAMAOKA Minoru (2003)  Matsumoto Dental University, School of Dentistry, Professor, 歯学部, 教授 (50064671)

高橋 悦治 (2002)  松本歯科大学, 歯学部, 助手 (60278174)

柴 肇一 (2000-2001)  北海道大学, 大学院・工学研究科, 助教授 (60241303)

Co-Investigator(Kenkyū-buntansha) SHIBA Toshikazu  Fujirebio Inc., Research and Development, Research Director, 先端研究部, リサーチディレクター (60241303)
TANAKA Hitoshi  Matsumoto Dental University, School of Dentistry, Assistant Professor, 歯学部, 講師 (50267788)
UEMATSU Takashi  Matsumoto Dental University, Graduate School of Oral Medicine, Associate Professor, 大学院・歯学独立研究科, 助教授 (40203476)
ERATA Tomoki  Hokkaido University, Faculty and Graduate School of Engineering, Associate Professor, 大学院・工学研究科, 助教授 (30213581)
山岡 稔  松本歯科大学, 歯学部, 教授 (50064671)
田島 健次  北海道大学, 大学院・工学研究科, 助手 (00271643)
高井 光男  北海道大学, 大学院・工学研究科, 教授 (50002019)
高橋 悦治  松本歯科大学, 口腔顎顔面外科講座, 助手 (60278174)
棟方 正信  北海道大学, 大学院・工学研究科, 教授 (50261326)
西村 和晃  大阪歯科大学, 歯学部, 講師
Project Period (FY) 2000 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥11,200,000 (Direct Cost: ¥11,200,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2002: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2001: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2000: ¥3,200,000 (Direct Cost: ¥3,200,000)
Keywordspolyphosphate / artificial periodontal membrane / tissue regeneration / periodontal tissue / osteoblast / 歯根膜 / MC3T3-E1 / 歯周病 / 組織再生 / 抗菌 / 抗炎症 / 歯周炎 / 歯周組織再生 / 骨再生 / 生体適合性材料 / 再生医療 / 細胞増殖促進 / 骨分化 / 骨分化促進
Research Abstract

Inorganic polyphosphates [Poly(P)] are chains of phosphate molecules and are often distributed in osteoblasts. The purpose of this study was to clarify the effects of Poly(P) on periodontal regeneration. The osteoblast-like cell line MC 3T3-E1 was cultured with Poly(P), and its gene expression and potential mineralization were evaluated by reverse-transcription polymerase chain reaction and von Kossa staining. Alkaline phosphatase activity was also determined. The potential role of Poly(P) in bone formation was assessed using a rat alveolar bone regeneration model. Poly(P) exhibited antibacterial activity against Prophyromonas gingivalis and Streptococcus mutans, induced expression of osteopontin and osteocalcine and increased alkaline phosphatase activity in MC 3T3-E1 cells. Promotion of alveolar bone regeneration was observed locally in Poly(P)-treated rats. These findings suggest that Poly(P) plays roles of osteoblastic differentiation, activation and bone mineralization and possesses antibacterial activity. Thus, local delivery of poly(P) may have adjunctive therapeutic benefits in periodontal disease.

Report

(5 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Shiba T.: "Inorganic polyphosphate and polyphosphate kinase : their novel functions and applications"Biochemistry. 65. 315-323 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsutsumi K.: "Involvement of inorganic polyphosphate in expression of SOS genes"Biochim.Biophys.Acta. 1493. 73-81 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Uematsu T.: "Multidrug resistance gene 1 expression in salivary gland"International Journal of Cancer. 92. 187-194 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishige K.: "Novel method for enzymatic synthesis of CMP-NeuAc"Biosci.Biotechnol.Biochem.. 65. 1736-1740 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kameda A.: "A novel regeneration system using polyphosphate-AMP phosphotransferase and polyphosphate kinase"J.Biosci.Biotechnol.. 9. 557-563 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaoka M.: "Relationship between third molar development and root angulations"J.Oral Rehabil. 28. 198-205 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaoka M.: "Alteration of the angle of the coronoid process in prognathism"J.Oral Rehabil. 28. 479-484 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Uematsu T.: "P-glycoprotein expression in the major and minor salivary glands"Archive of Oral Biology. 46. 521-527 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaoka M.: "Relationship of the hyoid bone and posterior surface of the tongue in prognathism and macrognathia"Journal of Oral Rehabilitation. 30. 914-920 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Uematsu T.: "Expression of ATP-binding cassette transporter in human salivary ducts."Archive of Oral Biology. 48. 87-90 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsuura T.: "Effect of salivary gland adenocarcinoma cell-derived alpha-N-acetylgalactosaminidase on the bioactivity of macrophage activating factor."Int J Oncol.. 24. 521-528 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Uematsu T.: "Effects of oral squamous cell-derived TGFβ1on CD26/DPPIV in T cells."Anticancer Research. 24. 619-624 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shiba T.: "Effect of inorganic polyphosphate on periodontal regeneration"Key Engineering Material. 245-256. 1119-1122 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shiba T.: "Inorganic polyphosphate and polyphosphate kinase : their novel functions and applications"Biochemistry(Moscow). No.65(3). 315-323 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsutsumi K.: "Involvement of inorganic polyphosphate in expression of SOS genes"Biochim. Biophys. Acta. Vol.1493(1-2). 73-81 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Uematsu T.: "Multidrug resistance gene 1 expression in salivary gland adenocarcinomas and oral squamous cell carcinomas"International Journal of Cancer. Vol.92 No.2. 187-194 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishige K.: "Novel method for enzymatic synthesis of CMP-NeuAc."Biosci. Biotechnol. Biochem. Vol.65(8). 1736-1740 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kameda A.: "A novel regeneration system using polyphosphate-AMP phosphotransferase and polyphosphate kinase"J. Biosci. Biotechnol.. Vol.9. 557-563 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaoka M.: "Relationship between third molar development and root angulations"Journal of Oral Rehabilitation. Vol.28. 198-205 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaoka M.: "Alteration of the angle of the coronoid process in prognathism"Journal of Oral Rehabilitation. Vol.28. 479-484 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Uematsu T.: "P-glycoprotein expression in the major and minor salivary glands"Archive of Oral Biology. Vol.46 No.6. 521-527 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaoka M.: "Relationship of the hyoid bone and posterior surface of the tongue in prognathism and macrognathia"Journal of Oral Rehabilitation. Vol.30 No.9. 914-920 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Uematsu T.: "Expression of ATP-binding cassette transporter in human salivary ducts."Archive of Oral Biology. Vol.48 No.1. 87-90 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsuura T.: "Effect of salivary gland adenocarcinoma cell-derived alpha-N-acetylgalactosaminidase on the bioactivity of macrophage activating factor."Int J Oncol.. Vol24. No.3. 521-528 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Uematsu T.: "Effects of oral squamous cell-derived TGF-β1on CD26/DPPIV in T cells."Anticancer Res.. Vol.24. 619-624 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shiba T.: "Effect of inorganic polyphosphate on periodontal regeneration"Key Engineering Material. Vol.245-256. 1119-1122 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shiba T., et al.: "Effect of inorganic polyphosphate on periodontal regeneration"Key Engineering Material. 245・256. 1119-1122 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 上松隆司: "歯周組織の再生医療"生物工学会誌. 80・5. 208-208 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K. Ishige, et al.: "Novel method for enzymatic synthesis of CMP-NeuAc"Biosci. Biotechnol. Biochem.. 65. 1736-1740 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] A. Kameda, et al.: "A novel regeneration system using polyphsophate-AMP phosphotransferase and polyphosphate kinase"J.Biosci.Biotechnol.. 9. 557-563 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M. Yamaoka, et al.: "Relationship between third molar development and root angulation"J. Oral Rehabil.. 28. 198-205 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M. Yamaoka, et al.: "Alteration of the angle of the coronoid process in prognathism"J. Oral Rehabil.. 28. 479-484 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T. Uematsu, et al.: "P-glycoprotein expression in human major and minor salivary glands"Archives of Oral Biology. 46. 521-527 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T. Uematsu, et al.: "Multidrug resistance gene I expression in salivary gland adenocarcinomas and oral squamous-cell carcinomas"Int. J. Cancer. 92. 187-194 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K, Tsutsumi, T.Shiba, et al.: "Involvement of inorganic polyphosphate in expression of SOS genes."Biochim.Biophys.Acta.. 1493. 73-81 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Shiba, K.Tsutsumi, K.Ishige, T.Noguchi: "Inorganic polyphosphate and polyphosphate kinase : their novel functions and applications."Biochemistry(Moscow). 65. 315-323 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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