| Project/Area Number |
12558110
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
OKAMOTO Koji Kyushu Institute of Technology, Department of Biochemical Engineering and Science, Professor, 情報工学部, 教授 (40122618)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Iori Kyushu Institute of Technology, Department of Biochemical Engineering and Science, Research Associate, 情報工学部, 助手 (50311858)
KAIBARA Kozue Kyushu University, Faculty and Graduate School of Science, Research Associate, 大学院・理学研究院, 助手 (90080564)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥8,400,000 (Direct Cost: ¥8,400,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2000: ¥4,400,000 (Direct Cost: ¥4,400,000)
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| Keywords | Elastin-derived peptide / Self-assembly / Phosphorylation / Auto-conversion / Coacervate droplet / Deassembly / Microparticle / エラスチン / 分子集合 / 薬物放出制御システム |
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| Research Abstract |
In 2000 to 2002, a polymer based on the repeating pentapeptide sequence Val-Pro-Gly-Val-Gly(VPGVG) which possesses the property of self-assembly was synthesized. This polymer, poly(VPGVG), was characteristic of completely reversible self-assembly and deassembly. The syntheses of two random copolymers based on the VPGVG sequence described above and a peptide sequence Arg-Gly-Tyr-Ser-Leu-Gly(RGYSLG) which can be phosphorylated by cyclic AMP-dependent protein kinase or a peptide sequence Gly-Ser-Asn-His-Gly(GSNHG) in which Asp residue can be converted to Asp residue were performed but these syntheses were unsuccessful because of the unexpected formation of by-products. In 2003, we tried again the syntheses of the above random copolymers by changing the synthetic scheme and quantitative yields of two random copolymers were obtained. Each of these copolymers was characteristic of incompletely reversible self-assembly and deassembly. The size of coacervate droplets of poly(VPGVG) in self-assembly was 1〜2 μm and the release of drug from microparticles of poly(VPGVG) was controlled. The release of drugs from microparticles of two random copolymers described above is under investigation
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