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Development of synchrotron-radiation vacuum-ultraviolet circular dichroism spectrophotometer and its application for structural analysis of biomaterials

Research Project

Project/Area Number 12559007
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 広領域
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

GEKKO Kunihiko  Hiroshima Univ., Graduate School of Science, Professor, 大学院・理学研究科, 教授 (10023467)

Co-Investigator(Kenkyū-buntansha) OHMAE Eiji  Hiroshima Univ., Graduate School of Science, Research Associate, 大学院・理学研究科, 助手 (30284152)
KATAYANAGI Katsuo  Hiroshima Univ., Graduate School of Science, Associate Professor, 大学院・理学研究科, 助教授 (20291479)
TANIGUCHI Masaki  Hiroshima Univ., Graduate School of Science, Professor, 大学院・理学研究科, 教授 (10126120)
FUKAZAWA Tomoyuki  JASCO Corp. Spectroscopic Products Division, Manager, 第一技術部, 技術担当マネージャー
深澤 知行  日本分光(株), 第一技術部, 技術担当マネージャー(研究職)
尾島 典行  広島大学, 医学部, 助手 (20311805)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2001: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2000: ¥5,100,000 (Direct Cost: ¥5,100,000)
Keywordssynchrotron radiation / vacuum-ultraviolet / circular dichroism / spectrophotometer / structural analysis / biomaterials
Research Abstract

In order to analyze the structure of biomaterials based on the higher energy transition of chromophores, we constructed the vacuum-ultraviolet circular dichroism (VUVCD) spectrophotometer which is capable of measuring the CD spectra in the wavelength region of 310-140 nm under high vacuum in the solution, using the synchrotron radiation source (HiSOR) at Hiroshima University Synchrotron Radiation Center (HSRC). The signal-to-noise ratio of the spectra was improved by exchanging the grating mirror in the monochromator. The performance of the constructed system was confirmed to be satisfactory by monitoring the CD spectra of ammonium d-camphor-10-sulfonate. The VUVCD spectra of eight amino acids were published in Chemistry Letters, 2002, 826. Further we constructed the temperature control unit of the sample cell with a Peltier thermoelectric element to keep the sample in the temperature range from -30 to 70℃. This system was applied for studying the cold-denaturation of myoglobin and the performance of the system were published in Analytical Sciences 19, 129 (2003). Usefulness of the constructed spectrophotometer was further confirmed from the measurements and theoretical assignment of VUVCD spectra of various sugars and proteins. This VUVCD spectrophotometer will be opened for common utilization from April of 2003.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Koichi Matsuo: "Vacuum-Ultraviolet Circular Dichroism of Amino Acids as Revealed by Synchrotron Radiation Spectrophotometer"Chemistry Letters. 2002. 826-827 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koichi Matsuo: "Optical Cell with a Temperature-Control Unit for a Vacuum Ultraviolet Circular Dichroism Spectrophotometer"Analytical Sciences. 19. 129-132 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koichi Matsuo: "Vacuum-Ultraviolet Circular Dichroism of Amino Acids as Revealed by Synchrotron Radiation Spectrophotometer"Chemistry Letters. 2002. 826-827 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koichi Matsuo: "Optical Cell with a Temperature-Control Unit for a Vacuum Ultraviolet Circular Dichroism Spectrophotometer"Analytical Sciences. 19. 129-132 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koichi Matsuo: "Vacuum-Ultraviolet Circular Dichroism of Amino Acids as Revealed by Synchrotron Radiation Spectrophotometer"Chemistry Letters. 2002. 826-827 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Koichi Matsuo: "Optical Cell with a Temperature-Control Unit for a Vacuum Ultraviolet Circular Dichroism Spectrophotometer"Analytical Sciences. 19. 129-132 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] N.Ojima: "Vacuum-ultraviolet circular dichroism spectrophotometer using synchrotron radiation : On-line performance"Chemistry Letters. 522-523 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Noriyuki Ojima: "Vacuum-ultraviolet circular dichroism spectrophotometer using synchrotron radiation : Optical system and off-line performance"Chemistry Letters. 832-833 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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