| Project/Area Number |
12575028
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
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Principal Investigator |
FUJISAKI Kozo Obihiro University of Agriculture and Veterinary Medicine (OUAVM), National Research Center for Protozoan Diseases (NRCPD), Professor, 原虫病研究センター, 教授 (00292095)
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| Co-Investigator(Kenkyū-buntansha) |
NAGASAWA Hideyuki OUAVM, NRCPD, Professor, 原虫病研究センター, 教授 (60172524)
XUAN Xuenan OUAVM, NRCPD, Associate Professor, 原虫病研究センター, 助教授 (10292096)
IGARASHI Ikuo OUAVM, NRCPD, Professor, 原虫病研究センター, 教授 (80159582)
KAMIO Tsugihiko OUAVM, NRCPD, Visiting Professor, National Institute of Animal Health, Head, 動物衛生研究所, 研究室長
INOUE Noboru OUAVM, NRCPD, Assistant Professor, 原虫病研究センター, 講師 (10271751)
筏井 宏実 北里大学, 獣医畜産学部, 助手
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2002: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 2001: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2000: ¥5,000,000 (Direct Cost: ¥5,000,000)
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| Keywords | piroplasmosis / Theileria orientalis sergenti / herpes virus / recombinant vaccine / Babesia parasites / gene cloning / vector tick / Haemaphysalis longicornis / ピロプラズマ病 / タイレリア原虫 / バベシア原虫 / 血清抗体 / 媒介ダニ / DNA診断 / 住血原虫病 / ELISA / カピパラ / トリパノソーマ |
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| Research Abstract |
(1) Serum samples from horses in the States of Sao Paulo and Mato Grosso do Sul, Brazil were examined for diagnosis of equine piroplasmosis by both the latex agglutination test (LAT) and the enzyme-linked immunosorbent assay (ELIZA) with recombinant antigens. Of the examined samples, 81% and 90% samples were positive for Babesia equi and Babesia caballi infection, respectively. Also, 75%75% samples were positive for both B. equi and B. caballi mixed infections. These results indicate that equine piroplasmosis is widespread and therefore a cause for serious concern in the States of Sao Paulo and Mato Grosso do Sul, Brazil.
(2) The potential role of Boophilus microplus as natural tick vector of B. equi and B. caballi in Brazilian horses was assessed using nested polymerase chain reaction (PCR)-based marker assay. B. equi merozoite-specific 218bp gene fragment (EMA-1) was detected in 45.3-62.5% of females, eggs, larvae, and nymphs of B. microplus collected from 47 horses at the State of Ma
… More
to Grosso do Sul, Brazil.
Except for the partially-fed female ticks, the B. caballi-specific 430 bp gene fragment was amplified from developmental stages. Therefore, we have obtained molecular evidence that strengthens earlier experimental and ultrastructural studies on Brazil incriminating B. microplus as a natural vector of B. equi, and possibly of B. caballi.
(3) Primers specific for EMA-1 gene of B. equi successfully amplified products from all samples of Dermacentor nuttalli adult ticks from Mongolia while primers for the 430 bp gene fragment product from BC48 gene of B. caballi amplified products from seven of the 54 samples. We have found mixed infections with B. equi and B. caballi in the tick vector. These results suggest that D. nuttalli may play an important role as a vector of both B. caballi and B. equi and also may be important in maintenance of endemicity of equine piroplasmosis in Mongolia. The detection of parasite DNA in eggs and larval progenies is likewise suggestive of transovarial parasite transmission in D. nuttalli ticks.
(4) At least three different variations in EMA-1 gene of B. equi strains isolated from different regions. The phylogenetic tree deduced from the sequences suggests a closer relationship between the Brazilian and Florida strains of B. equi DNA, and a distant relationship between B. equi DNA isolated from the Brazilian samples and USDA strain. B. equi gene obtained from Mongolian samples revealed comparatively distant relationship to other examined strains. Less
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