| Budget Amount *help |
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2001: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2000: ¥8,500,000 (Direct Cost: ¥8,500,000)
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| Research Abstract |
Villous malignant change brings about hydatidiform mole or choriocarcinoma with the change of sugar chain structures and bioactivity of hCG, As In Japan, the cases of these diseases have been decreasing; we have constructed the cooperative research projects, as a counterpart, with the Department of Internal Medicine Mahidol University Medical School, Thai Land. However, the number of the studied cases is not so many. Sugar chain structures had been analyzed by Isoelectrofocussing, sialic acid content, deglycosidase study, and affinity study to lectins. In addition, the gene expression of glycosyl-transferases was analyzed. These diseases are reported to show severe hyperthyroidism. Our cases were, however, divided into hyperthyroidism and not hyperthyroidism groups. Isoelectrofocussing disclosed that the cases with much hCG molecules with neutral regions showed hyperthyroidism and those with strong acidic regions did not show that. Sialidase treatment induced the acidic molecules moving into the neutral region, suggesting the hCG molecules of acidic regions having much sialic acid molecules. Similarly, RT-PCR study confirmed that the villi with much acidic region molecules expressed much expression of sialidase genes.
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