| Project/Area Number |
12640564
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
機能・物性・材料
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| Research Institution | Chuo University |
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Principal Investigator |
CHIKIRA Makoto Chuo University,Faculty of Science and Engineering, Professor, 理工学部, 教授 (70006328)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | DNA fiber ESR / DNA cleaving reaction / phenathroline copper(II) complex / phenathroline Pt(II) complex / NMR / oligonucleotide / Ni(III) complex / tripeptide / DNA / フェナントロリン白金(II)錯体 / ペプチドNi(III)錯体 / 金属錯体 / ペプチド銅(II)錯体 / ポリアミン銅(II)錯体 / デグリコペプロマイシン / Ni(III)錯体 |
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| Research Abstract |
In order to develop effective reagents that cleave DNA oxidatively or hydrolytically, various metal complexes have been synthesized and the reactivity and the DNA binding structures have been investigated.
1. Several copper(II) complexes with 4-dentate phenanthroline derivatives with substituents at 2- and 9-postions were synthesized. Bulky substituents that deformed the planarity of the complex enhanced the oxidative cleavage of DNA and the correlation between the reactivity and the binding structure was discussed.
2. In the cleaving reaction with ternary copper(II) complexes with phenanthroline and amino acid, the amino acid depresses the reaction. Further lowering of the reactivity was induced by 2,9-dimethyl substituted phenanthroline. These results suggested that cleaving reaction proceeds with the mono-phenanthroline copper(II) complex.
3. Binding structures of the ternary platinum(II) complexes of phenanthroline or dipyridine with lysine bound to oligonucleotide DO1 and DO2 were analyzed by NMR. The phenanthroline derivative intercalates between G2 and C3 of DO1 and G4 and A5 of DO2 from the minor groove while the bipyridine derivative binds to the AT site of DO1 in the minor groove. The binding structures were refined by molecular dynamic calculation.
4. ESR signals from unstable Ni(III) complexes of histidine-containing tripeptides could be detected by in-situ oxidation of the Ni(II) complex incorporated in DNA. The super-hyperfine splitting from nitrogenous base suggested a presence of the species coordinated to a guanine N7.
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