| Project/Area Number |
12640590
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
分離・精製・検出法
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
KITAO Fujiwara Tokyo University of Pharmacy and Life Science, School of Life Science, Professor, 生命科学部, 教授 (90090521)
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| Co-Investigator(Kenkyū-buntansha) |
HIDETOSHI Kumata Tokyo University of Pharmacy and Life Science, School of Life Science, Instructor, 生命科学部, 助手 (60318194)
TERUAKI Sakurai Tokyo University of Pharmacy and Life Science, School of Life Science, Instructor, 生命科学部, 助手 (30266902)
TOSHIKAZU Kaise Tokyo University of Pharmacy and Life Science, School of Life Science, Professor, 生命科学部, 教授 (20266894)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥3,500,000 (Direct Cost: ¥3,500,000)
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| Keywords | solar radiation / photochemical reaction / active oxygen / natural water / seawater / hydrogen peroxide / superoxide negative / humic acid / 活性酸素 / NADH / フローインジェクション法 / ニトロブルーテトラゾリウム / 海洋表層 / ポルフィクン錯体 / 水和電子 |
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| Research Abstract |
It is considered that the active oxygen species generate in the surface of natural waters under irradiation of the sun. In coastal seawater which contains abundant organic substance, hydrogen peroxide, one of the major active oxygen, generates up to 300 nM by the solar irradiation. OH radical, superoxide negative ion, singlet oxygen are the active oxygen species other than hydrogen peroxide. However, these species have much shorter life time compared with hydrogen peroxide, and difficult to determine. We investigated the methods to determine OH radical and superoxide negative ion in river and seawater after irradiation of solar simulator. OH radical is detected by means of conversion rate of benzene to phenol and separated by HPLC. In the case of superoxide negative ion, lactate dehydrogenase-NADH reaction, nitroblue-tetrazolium method, and nitromethane method were investigated. However, deionized water produce more superoxide negative ion than river water after the irradiation of solar simulator. Flow injection type chemiluminescence detector was constructed and chemiluminescence was detected after alkaline luminol and superoxide negative ion. In this case, humic acid gave a strong chemiluminescence compared with deionized water after irradiation of solar simulator.
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