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Molecular Genetic Analyses of the Role of the Major Acidic Phospholipids in Escherichia coli Cell Growth

Research Project

Project/Area Number 12640595
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 遺伝
Research InstitutionSaitama University

Principal Investigator

MATSUMOTO Kouji  SAITAMA UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, PROFESSOR, 理学部, 教授 (00119140)

Co-Investigator(Kenkyū-buntansha) HARA Hiroshi  SAITAMA UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, ASSOCIATE PROFESSOR, 理学部, 助教授 (00173071)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsEscherichia coli / Acidic phospholipids / phosphatidylglycerol / lipoprotein / rcs mutation / 酸性リン脂質 / yojN変異 / プロリポ蛋白質
Research Abstract

Two plans were enforced. They were resulted from the discovery that E. coli cells lacking the major acidic phospholipids were viable if it harbored a defective lpp allele.
1) One is to clarify the lethality of acidic-phospholipid deficiency in the cells having wild type lpp allele. The proLpp expressed from a plasmid caused cell lysis in a pgsA lpp double mutant. The envelopes of the cells could not be separated into outer and inner membranes. In contrast, expression of a mutant Lpp lacking the C-terminal lysine residue (required for linking to peptidoglycan) did not cause lysis and allowed the clear separation of the outer and inner membranes. In pgsA mutants proLpp could not be modified by the addition of a diacylglyceryl moiety normally provided by phosphatidylglycerol and that this defect caused unmodified proLpp to The accumulation of unmodified wild type Lpp led to the linking to peptidoglycan, causing the inner membrane to be anomalously anchored to peptidoglycan and leading to cell lysis. This anomalous anchoring explains the non-viable phenotype of pgsA null mutants.
2) The other is to clarify the reason for the temperature-sensitivity of the cells lacking the major acidic phospholipids. For this purpose we isolated 48 clones of temperature-resistant suppressor mutants and identified almost all the loci of suppressor mutations. They were revealed to have mutations in the genes responsible for rcs phosphorelay system (rcsC, rcsB, rcsF, yojN, and rcsA). An over-expression of a certain gene (s) under the control of rcs regulatory system might be responsible for the temperature-sensitivity.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] MATSUMOTO, K.: "Dispensable nature of phosphatidylglycerol in Escherichia coli : dual role of anionic phospholipids"Molecular Microbiology. 39(6). 1427-1433 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 鈴木基生, 原 弘志, 松本幸次: "大腸菌主要酸性リン脂質欠損変異の致死性をlpp変異がサプレスする機構"脂質生化学研究[Proceedings of Japanese Conference on the Biochemistry of Lipids (JCBL)]. 43. 21-24 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] SUZUKI, M., H.HARA, K.MATSUMOTO: "Envelope disorder of Escherichia coli cells lacking phosphatidylglycerol"J. Bacteriol.. 184(19). 5418-5425 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 河合文隆, 正田桃子, 原島里恵, 原 広志, 松本幸次: "カルジオリピンの枯草菌細胞内局在"脂質生化学研究[Proceedings of Japanese Conference on the Biochemistry of Lipids (JCBL)]. 44. 109-112 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsumoto, K: "Dispensable nature of phosphatidylglycerol in Escherichia coli : dual role of anionic phospholipids"Molecular Microbiology. 39(6). 1427-1433 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suzuki, M., H. Hara, and K. Matsumoto: "Suppression of the lethality of the mutation that causes lack of the major acidic phospholipids in Escherichia coli"Proceedings of Japanese Conference on the Biochemistry of Lipids (JCBL). 43. 21-24 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suzuki, M., H, Hara, and K. Matsumoto: "Envelope disorder of Escherichia coli cells lacking phosphatidylglycerol"J. Bacteriol.. 184(19). 5418-5425 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawai, F., Shoda, M., Harashima, R., H. Hara, and K, Matsumoto: "Visualization of cardiolipin domains in Bacillus subtilis Marburg"Proceedings of Japanese Conference on the Biochemistry of Lipids (JCBL). 44. 109-112 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsumoto, K.: "Dispensable nature of phosphatidylglycerol in Escherichia coli:dual roles of anionic phospholipids"Molec.Microbiol.. 39(6). 1427-1433 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 鈴木基生, 原 弘志, 松本幸次: "大腸菌主要酸性リン脂質欠損変異の致死性を1pp変異がサプレスする機構"脂質生化学研究(JCBL). 43. 21-24 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsumoto,K.,: "Dispensable nature of phosphatidylglycerol in Escherichia coli : dual roles of anionic phospholipids."Molecular Microbiology.. 39(6). 1427-1433 (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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