| Project/Area Number |
12640628
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
植物生理
|
|---|
| Research Institution | CHIBA UNIVERSITY |
|---|
Principal Investigator |
KODAMA Hirotaki Faculty of Horticulture, Chiba University, Associated professor, 園芸学部, 助教授 (70302536)
|
|---|
| Project Period (FY) |
2000 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥2,300,000 (Direct Cost: ¥2,300,000)
|
|---|
| Keywords | post-transcritional gene silencing / fatty acid desaturase / α-linolenic acid / RNA processing / translational regulation / ジーンサイレンシング / 転写後抑制 / スプライシング / リノレン酸合成酵素 / タバコ / 形質転換植物 / 遺伝子導入 |
|---|
| Research Abstract |
A tobacco endoplasmic reticulum ω-3 fatty acid desaturase (NtFAD3) catalyzes the formation of α-linolenate. A transgenic tobacco plant (S44) over-producing the NtFAD3 mRNA exhibited an extremely low α-linolenate level in leaves, but in root tissues α-linolenate accumulated successfully. In the S44 leaves the RNAs for introduced NtFAD3 gene accumulated in a gene-dosage-dependent manner, suggesting that in the silenced leaves of the S44 homozygous plants the transgene's expression is suppressed at the (post)translational steps. The processing of nascent RNAs for endogenous NtFAD3 and the plastid isozyme gene was severely inhibited in the nuclei isolated from S44 leaves, which was followed by degradation of these mRNAs. These data indicate that block of the ω-3 desaturation step is achieved by multiple silencing processes.
|
